Two new species of trypanosomes (subgenus <i>Schizotrypanum</i>) in bats from southern Ontario

Bower, Susan M.; Woo, P. T. K.

doi:10.1139/z81-077

Cited by 26 publications

(12 citation statements)

References 27 publications

(44 reference statements)

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“…The lack of regional MHC differentiation in M. lucifugus across its northern range suggests that disease-related selective pressures on this species may be similar among genetically differentiated clusters, but testing this hypothesis requires more robust genetic tools that can differentiate among duplicated loci, and that can simultaneously target multiple immune genes. Bats in our sampling area coexist with a variety of pathogens including several strains of bat rabies (Middleton et al 2015), corona and polyoma viruses (Misra et al 2009), endemic fungal pathogens such as Trichophyton redelli (Lorch et al 2015), internal parasites such as Trypanosoma myoti (Bower and Woo 1981), and ectoparasites such as fleas and mites (Webber et al 2015). Most of these are relatively widespread, affecting bats across North America.…”

Section: Davy Et Almentioning

confidence: 99%

Prelude to a panzootic: Gene flow and immunogenetic variation in northern little brown myotis vulnerable to bat white-nose syndrome

Davy

Donaldson

Rico

et al. 2017

FACETS

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The fungus that causes bat white-nose syndrome (WNS) recently leaped from eastern North America to the Pacific Coast. The pathogen's spread is associated with the genetic population structure of a host (Myotis lucifugus). To understand the fine-scale neutral and immunogenetic variation among northern populations of M. lucifugus, we sampled 1142 individuals across the species' northern range. We used genotypes at 11 microsatellite loci to reveal the genetic structure of, and directional gene flow among, populations to predict the likely future spread of the pathogen in the northwest and to estimate effective population size (N e ). We also pyrosequenced the DRB1-like exon 2 of the class II major histocompatibility complex (MHC) in 160 individuals to explore immunogenetic selection by WNS. We identified three major neutral genetic clusters: Eastern, Montane Cordillera (and adjacent sampling areas), and Haida Gwaii, with admixture at intermediate areas and significant substructure west of the prairies. Estimates of N e were unexpectedly low (289-16 000). Haida Gwaii may provide temporary refuge from WNS, but the western mountain ranges are not barriers to its dispersal in M. lucifugus and are unlikely to slow its spread. Our major histocompatibility complex (MHC) data suggest potential selection by WNS on the MHC, but gene duplication limited the immunogenetic analyses.

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Section: Davy Et Almentioning

confidence: 99%

Prelude to a panzootic: Gene flow and immunogenetic variation in northern little brown myotis vulnerable to bat white-nose syndrome

Davy

Donaldson

Rico

et al. 2017

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“…For detailed morphological studies, thin smears of free trypanosomes in culture media were air dried, fixed in absolute methanol and then in 10~o buffered formalin, stained with Giemsa's stain, drawn and measured (Woo, 1969a;Bower & Woo, 1981a). To study the intracellular stages within fibroblasts, cell cultures were washed with phosphate buffered saline warmed to 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Knowledge of T. dionisii from Europe and T. vespertilionis, believed to be cosmopolitian, is mostly based on culture forms which are distinct (Baker & Thompson, 1971). Recently, two additional species, T. hedricki and T. myoti were described from bats in North America (Bower & Woo, 1981a). The morphology of blood forms and tissue stages, and the host specificity and transmission cycle of the two later species were studied in detail (Bower & Woo, 1981a, 1981b.…”

Section: Introductionmentioning

confidence: 99%

An in vitro comparison of Trypanosoma spp. (subgenus Schizotrypanum) from bats

Bower

Woo

1981

Syst Parasitol

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Epimastigotes of Trypanosoma vespertilionis from diphasic blood agar cultures were on the average longer and the distance between the nucleus and kinetoplast greater than epimastigotes of T. hedricki, T. myoti and T. dionisii. Also, no yellow granules were seen in the epimastigotes of T. vespertilionis whereas they were obvious in the other three species. Long thin trypomastigotes which are characteristic of T. hedricki, T. myoti and T. dionisii cultures were not seen in T. vespertilionis.T. dionisii was much less infective to fibroblasts from mice and did not develop in fibroblasts from chicken, as did T. hedricki and T. myoti. Blood trypomastigotes were seen in chicken embryos inoculated with blood agar cultures of T. hedricki and T. myoti, but none was seen in embryos infected with T. dionisii.The cultural characteristics examined could not be used to differentiate T. hedricki from T. myoti.

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“…In the following example, a file is created for Trypanosoma myoti, a species of Schizotrypanum described from the small brown bat (Bower & Woo, 1981). Its descriptive code is MZA157XH.805.…”

Section: Procedures For Entering Data Into Computermentioning

confidence: 99%