Comparative Diagnosis of Infectious Bacteria in Bovine Milk

Sedky, Doaa; Ghazy, Alaa A.; Soliman, N. A. M.; Shaapan, Raafat M.

doi:10.17582/journal.jahp/2020/8.4.171.182

Cited by 4 publications

(5 citation statements)

References 45 publications

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“…Bovine milk is an important food source for humans and calves with its nutritive contents and probiotic microorganisms. However, it can also be a source of transmission for some foodborne diseases [ 18 , 19 ]. When first obtained, milk always has a microbial flora whose importance in terms of health is determined by the diversity of species and some characteristics of the species it includes [ 20 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

The Characterization and Beta-Lactam Resistance of Staphylococcal Community Recovered from Raw Bovine Milk

et al. 2023

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Staphylococci is an opportunistic bacterial population that is permanent in the normal flora of milk and poses a serious threat to animal and human health with some virulence factors and antibiotic-resistance genes. This study was aimed at identifying staphylococcal species isolated from raw milk and to determine hemolysis, biofilm, coagulase activities, and beta-lactam resistance. The raw milk samples were collected from the Düzce (Türkiye) region, and the study data represent a first for this region. The characterization of the bacteria was performed with MALDI-TOF MS and 16S rRNA sequence analysis. The presence of coa, icaB, blaZ, and mecA was investigated with PCR. A nitrocefin chromogenic assay was used for beta-lactamase screening. In this context, 84 staphylococci were isolated from 10 different species, and the dominant species was determined as S. aureus (32.14%). Although 32.14% of all staphylococci were positive for beta hemolysis, the icaB gene was found in 57.14%, coa in 46.42%, mecA in 15.47%, and blaZ in 8.33%. As a result, Staphylococcus spp. strains that were isolated from raw milk in this study contained some virulence factors at a high level, but also contained a relatively low level of beta-lactam resistance genes. However, considering the animal–environment–human interaction, it is considered that the current situation must be monitored constantly in terms of resistance concerns. It must not be forgotten that the development of resistance is in constant change among bacteria.

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Section: Discussionmentioning

confidence: 99%

The Characterization and Beta-Lactam Resistance of Staphylococcal Community Recovered from Raw Bovine Milk

et al. 2023

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“…Raw bovine milk is considered as a vehicle for many infectious bacterial diseases such as Bovine Tuberculosis, Brucellosis, Leptospirosis, Q. fever and Mycoplasma infection. In addition to, Infectious bacteria associated with mastitis including Staphylococcus aureus, coagulase negative staphylococci, Streptococcus dysgalactiae., Streptococcus agalactiae, Corynebacteriun pyogenes, Streptococcus uberis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Streptococcus pyogenes, Clostridium perfringens and Salmonella species were also included [19]. Mastitis organisms differ from one area to another.…”

Section: Mastitis Causing Bacteria and Global Incidencementioning

confidence: 99%

“…Strategies for SCM detection might be helpful in reducing the likelihood of infection propagation. Traditional field tests like somatic cell count, California mastitis test, electrical conductivity, and pH tests have many benefits since they are practical, affordable, and useful for tracking the health of cattle's udders [19].…”

Section: Recent Fast and Precise Diagnostic Toolsmentioning

confidence: 99%

Advances in Controlling Bacterial Mastitis in Dairy Cows

Elbayoumy,

Allam,

Ghazy

et al. 2024

Egyptian Journal of Veterinary Sciences

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M ASTITIS is the most world frequent and costly disease in dairy cattle. Although several infectious agents such as bacteria, Mycoplasma, and fungi have been associated to mastitis, bacteria are the primary important cause. Improved milking hygiene, implementation of post-milking teat disinfection, and maintenance of milking machinery are all general methods to avoid new cases of mastitis, but antibiotics are the primary treatment to control mastitis infection. Antibiotic residue and antimicrobial resistance, as well as the effect of antibiotic abuse on public health, have led to several limits on uncontrolled antibiotic therapy in the dairy industry across the world. New therapeutic techniques to substitute antibiotics in the control of mastitis have been examined by researchers. These efforts, which were aided by the significant advancements in nanotechnology, stem cell assays, molecular biology tools, and genomics, led to the creation of innovative mastitis treatment and control methods. In the present study, unique mastitis management approaches are discussed, including the breeding of mastitis-resistant dairy cows, the creation of novel diagnostic and treatment techniques, and the use of contemporary mastitis vaccines, cow drying protocols, teat sterilisation, housing, and nutrition. Besides, it include the use of nanotechnology, stem cell technologies, immunotherapy, traditional herbal medicine plants, nutraceuticals, probiotics, bacteriocins, bacteriophages, and acoustic pulse technology.

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“…After clinical examination of udder, milk samples were aseptically collected as previously described by Amer et al (2018). California mastitis test (CMT) were applied for detection of clinical and subclinical mastitis (Sedky et al, 2020b). The quarter which gave positive result for CMT was considered as sub-clinically affected.…”

Section: Collection Of Milk Samplesmentioning

confidence: 99%

Assessment of Phytochemical, Antioxidant and Antibacterial Activity of Balanites Aegyptiaca and Curcuma Longa against Some Bacterial Pathogens Isolated from Dairy Cow Infected with Mastitis

Sedky¹,

Mohamed²,

Fouad³

et al. 2021

AAVS

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Bovine mastitis is very important disease threatening the dairy industry and animal wealth globally, resulting in great economic losses. The present work was aimed to assess the phytochemical components, antioxidant activity and the antibacterial effectiveness of Balanites aegyptiaca fruits (BAF) and Curcuma longa powder (CLP) extracts. A total of 287 quarter milk samples were collected from dairy cows raised in four different governorates of Egypt; 95 from clinical mastitis, 37 from subclinical mastitis and 155 from normal milk samples. The isolates from milk samples were identified and differentiated by microbiological cultures, colony morphology, hemolytic activity of the colony, Gram's stain and biochemical tests. Antibacterial activity of the aqueous extract of BAF (AE-BAF) and ethanolic extract of CLP (EE-CLP) at different concentrations were investigated against Gram-positive and Gram-negative bacteria isolated from clinical and subclinical mastitic cows using agar well diffusion technique. The isolated bacteria from collected milk samples were Staphylococcus aureus (30.30%), Escherichia coli (28.03%), Streptococcus agalactiae (19.70%), Salmonella spp. (10.60%), Bacillus subtilis (6.06%), Klebsiella pneumoniae (3.03%) and Pseudomonas aeruginosa (2.27%). Preliminary phytochemical screening of AE-BAF indicated the presence of flavonoids, saponins, tannins, phenols, carbohydrate, cardiac glycoside, terpenoids and steroids. Except the cardiac glycoside, the EE-CLP contains the same components. The antioxidant efficacy of the tested plant extracts was evaluated by using free radical scavenging assay method. The AE-BAF and EE-CLP posses 86.6% and 85.9% free radical scavenging activity with 1.5 and 0.125 mg /ml concentrations, respectively. The AE-BAF possess significant antibacterial activity at 400 and 800 mg/ml against all isolates, except K. pneumoniae which was not suppressed at the 400 mg/ml concentration. The significant (P ≤ 0.05) antibacterial activities of EE-CLP were observed at 20 and 50 mg/ml concentrations. It could be concluded that AE-BAF and EE-CLP exhibited potent in-vitro antibacterial activities, thus justifying their application in treatment of clinical cases of bovine mastitis.

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Comparative Diagnosis of Infectious Bacteria in Bovine Milk

Cited by 4 publications

References 45 publications

The Characterization and Beta-Lactam Resistance of Staphylococcal Community Recovered from Raw Bovine Milk

The Characterization and Beta-Lactam Resistance of Staphylococcal Community Recovered from Raw Bovine Milk

Advances in Controlling Bacterial Mastitis in Dairy Cows

Assessment of Phytochemical, Antioxidant and Antibacterial Activity of Balanites Aegyptiaca and Curcuma Longa against Some Bacterial Pathogens Isolated from Dairy Cow Infected with Mastitis

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