Comparative Concentration of Various Constituents of Blood Plasma and Dental-Pulp Fluid

Haldi, John; Law, Mary L.; John, Kathryn M.

doi:10.1177/00220345650440022001

Cited by 6 publications

(2 citation statements)

References 12 publications

(2 reference statements)

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“…It has been discussed that pulpal remineralisation effects might be more pronounced when remineralisation solutions rather than SPF are utilised, as the latter is only limitedly saturated with calcium and phosphate [Haldi et al, 1965]. We could not confirm such a difference, which might indicate that the process of pulpal remineralisation is not mainly controlled by the mineral saturation of the fluid, but the dentin distance and the applied pressure.…”

Section: Discussioncontrasting

confidence: 56%

Pulpal Remineralisation of Artificial Residual Caries Lesions in vitro

Schwendicke¹,

Al-Abdi²,

Meyer-Lückel

et al. 2015

Caries Res

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We assessed pulpal remineralisation of caries lesions in vitro. On the coronal aspect of human dentin discs (n = 70), artificial lesions [mineral loss ΔZ (mean ± SD) = 3,060 ± 604 vol% × µm] were induced, covered and pulpal surfaces exposed to pulpal fluid, remineralisation medium or water at pressures of 0, 1.47 or 2.94 kPa for 3 months. Mineral loss differences were assessed using transversal microradiography. No significant mineral gain occurred at 0 kPa and in samples exposed to water. At 1.47 and 2.94 kPa, pulpal and remineralisation fluid induced significant mineral gain [ΔΔZ = 1,317 (25th/75th percentiles: 735/1,541) vol% × µm; p < 0.001]. Pressure and fluid composition determined pulpal remineralisation in vitro.

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Section: Discussioncontrasting

confidence: 56%

Pulpal Remineralisation of Artificial Residual Caries Lesions in vitro

Schwendicke¹,

Al-Abdi²,

Meyer-Lückel

et al. 2015

Caries Res

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“…Dentinal fluid represents pulp IF collected from cut dentin surfaces. Data regarding its composition has been published (Haldi et al 1965; Coffey et al 1970; Knutsson et al 1994) but cutting the dentin surface and exposing the dentinal tubules induces inflammatory reactions in the tissue. In this study, we showed that it is possible to obtain pulp IF by centrifugation of pulp tissue at low G ‐force.…”

Section: Discussionmentioning

confidence: 99%

Cytokine signalling in rat pulp interstitial fluid and transcapillary fluid exchange during lipopolysaccharide‐induced acute inflammation

Bletsa¹,

Berggreen²,

Fristad³

et al. 2006

The Journal of Physiology

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The dental pulp consists of loose connective tissue encased in rigid dentinal walls. Because of its topography the tissue has low interstitial compliance and limited capacity to expand during fluid volume changes. Due to limitations regarding access to interstitial fluid, basic knowledge on transcapillary fluid transport parameters is lacking for this organ. The scope of this project was dual: first we aimed at establishing a method for isolation of pulp interstitial fluid (IF), and second we applied the method in rats subjected to lipopolysaccharide (LPS)-induced endotoxaemia. The aim was to measure colloid osmotic pressure (COP) and pro-inflammatory cytokines in the pulp IF during acute inflammation. Fluid volumes and pulpal blood flow (PBF) were measured to obtain more information about microcirculatory changes that take place in this pulpitis model. By centrifugation of incisor pulp at 239 g we were able to extract fluid representative for IF. Pulp IF had a relative high control COP (approximately 83% of plasma COP) and was similar to plasma COP 3 h after LPS challenge. The pulp exhibited a high content of IF (0.60 +/- 0.03 ml (g wet weight)(-1)) and a vascular volume of 0.03 +/- 0.01 ml (g w.w.)(-1) No differences were observed in the distribution of fluid volumes after 1.5 and 3 h LPS exposure. PBF and systemic blood pressure dropped significantly after LPS administration. PBF remained low whereas systemic blood pressure was re-established during the 3-h period, implying organ dysfunction. There was a differential pattern of cytokine expression in pulp IF and serum with cytokines such as IL-1alpha, IL-1beta and TNF-alpha locally produced, whereas others such as IFN-gamma and IL-6 were produced systemically and probably spilled over to the pulp IF after LPS exposure. Our findings show that pulp IF can be isolated by centrifugation and that this method is useful when studying fluid balance and extracellular signalling mechanisms in the dental pulp in normal and pathological conditions.

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