Fluid and protein flux across the pulpodentine complex of the dog in vivo

Maita, Eikichi; Simpson, Mark; Tao, L.; Pashley, David H.

doi:10.1016/0003-9969(91)90072-3

Cited by 61 publications

(35 citation statements)

References 19 publications

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“…5 It may be so that the cellulose membrane actively soaked more fluid from the dentin wound than the PVDF membrane did. Dentinal fluid flow is related to pulpal pressure.…”

Section: Discussionmentioning

confidence: 99%

“…6 Similar approaches were also used to study dentinal fluid flux in dog teeth. 5,11 These collection methods, however, are impracticable in the clinical situation. The current research aimed towards the development a chair-side assay that could reflect the health of pulp before a tooth is restored.…”

Section: Discussionmentioning

confidence: 99%

“…17 The idea to use PMN-associated enzymes as markers for pulpal health was formulated, yet not tested, more than 20 years ago. 5 In this context, the MMP system of enzymes is interesting. Some of these enzymes are produced by resident cells.…”

Section: Discussionmentioning

confidence: 99%

“…4 It is based on the collection and analysis of dentinal fluid. 5,6 However, the collection of dentinal fluid is not unproblematic. The fluid and protein flux across the pulpodentin complex is influenced by multiple factors.…”

Section: Introductionmentioning

confidence: 99%

“…The fluid and protein flux across the pulpodentin complex is influenced by multiple factors. 5 In addition, a simple and clinically useful tool for dentinal fluid collection is currently not available.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of vehicles to collect dentinal fluid for molecular analysis

Zehnder

Rechenberg

Bostancı

et al. 2014

Journal of Dentistry

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OBJECTIVES To test the hypothesis that a material with higher water absorption than polyvinylidene fluoride (PVDF) could increase the yield of target molecules from exposed dentine. METH-ODS In a series of standard tests, different cellulose membranes were compared to a PVDF counterpart for their ability to absorb water and release protein. In a subsequent randomized clinical trial, the cellulose material with the most favourable values was compared to PVDF regarding the levels of MMP-2 that could be collected from exposed dentine of healthy human teeth during filling replacement. MMP-2 levels were determined by enzyme-linked immunosorbent assay (ELISA). Data from the laboratory experiments were compared between materials using the appropriate parametric tests. The frequency of cases yielding quantifiable levels of MMP-2 was compared between materials by Fisher's exact test. The level of significance was set at 5%. RESULTS The cellulose membrane with the largest pore size (12-15 m) absorbed significantly (P<0.05) more water than PVDF. It showed a protein release that was similar to that of PVDF, while the cellulose membranes with smaller pore size retained significantly more protein (P<0.05). Using the large-pore cellulose membrane, MMP-2 could be collected at a quantifiable level from the dentine of healthy teeth in 9 of 13 cases, compared to 1 of 13 with the PVDF membrane (P<0.05). CONCLUSIONS Under the current conditions, a large-pore cellulose membrane yielded more of a molecule of diagnostic value compared to a standard PVDF membrane. CLINICAL SIGNIFICANCE Molecular diagnostics of dentinal fluid are hampered by low yields. In the current study, it was shown that cellulose membranes are more useful to collect MMP-2 from dentinal fluid than PVDF membranes. ABSTRACTObjectives: To test the hypothesis that a material with higher water absorption than polyvinylidene fluoride (PVDF) could increase the yield of target molecules from exposed dentin. Methods:In a series of standard tests, different cellulose membranes were compared to a PVDF counterpart for their ability to absorb water and release protein. In a subsequent randomized clinical trial, the cellulose material with the most favorable values was compared to PVDF regarding the levels of MMP-2 that could be collected from exposed dentin of healthy human teeth during filling replacement. MMP-2 levels were determined by enzyme-linked immunosorbent assay (ELISA). Data from the laboratory experiments were compared between materials using the appropriate parametric tests. The frequency of cases yielding quantifiable levels of MMP-2 was compared between materials by Fisher's exact test. The level of significance was set at 5%. Results:The cellulose membrane with the largest pore size (12 -15 µm) absorbed significantly (P < 0.05) more water than PVDF. It showed a protein release that was similar to that of PVDF, while the cellulose membranes with smaller pore size retained significantly more protein (P < 0.05). Using the large-pore cellulose membrane, MM...

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“…5 It may be so that the cellulose membrane actively soaked more fluid from the dentin wound than the PVDF membrane did. Dentinal fluid flow is related to pulpal pressure.…”

Section: Discussionmentioning

confidence: 99%