Comparative characterization of the hydrogel added PLA/β-TCP scaffolds produced by 3D bioprinting

Aydoğdu, Mehmet Onur; Öner, Ebru Toksoy; Ekren, Nazmi; Erdemir, Gökçe; Kuruca, Serap Erdem; Yuca, Esra; Bostan, Müge Sennaroglu; Eroğlu, Mehmet S.; Ikram, Fakhera; Uzun, Muhammet; Gündüz, Oğuzhan

doi:10.1016/j.bprint.2019.e00046

Cited by 35 publications

(38 citation statements)

References 40 publications

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“…It is particularly interesting that 5 out of these 10 papers utilized alginate and GelMA with other different components: hyaluronic acid methacrylated (Costantini et al, 2016 ), PEG (Daly et al, 2016b ), PEGDA (Kang et al, 2017 ), PEDGA/cellulose (García-Lizarribar et al, 2018 ), and PEGMA/agarose (Daly et al, 2016a ). In the same way, four hydrogels are composed by alginate and collagen with different components: gelatin (Bandyopadhyay et al, 2018 ), chitosan (Aydogdu et al, 2019 ), agarose (Yang et al, 2017 ), and gelatin/chitosan (Akkineni et al, 2016 ). Finally, other hydrogel is composed by alginate, hyaluronic acid, and cellulose (Nguyen et al, 2017 ).…”

Section: Resultsmentioning

confidence: 99%

“…However, its low mechanical properties and its slow gelation make chitosan a material rarely used alone. For this reason, to solve these poor mechanical properties it is usually combined with other materials as hyaluronic acid (Kim et al, 2019 ), alginate/collagen (Aydogdu et al, 2019 ), collagen/agarose (Campos et al, 2015 ), and alginate/gelatin/collagen (Akkineni et al, 2016 ). Chitosan also appears combined with silk (Zhang J. et al, 2018 ) and modified with hydroxybutil groups to improve its water solubility or with oxidized chondroitin sulfate to improve its mechanical properties (Li et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…Chemical cross-linking is mostly done to alginate (52 papers) with CaCl 2 solution (46 papers), and detailed concentrations can be seen in Figure 6 . Other (non-Ca 2+ ) solutions are used in two papers (Hafeez et al, 2018 ; Aydogdu et al, 2019 ) which use hydrazine and NaOH, respectively, and other two studies do not provide information (Aljohani et al, 2018 ; Yoon et al, 2019 ). Other specific cross-linking agents are genipin (Akkineni et al, 2016 ; Kim et al, 2016 ), mTgase (Tijore et al, 2018b ) or 1-ethyl-3-(3- dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) (Choi et al, 2018 ), and MAL-PEG-MAL (Yan et al, 2018 ) for gelatin, or different solute for other materials like thrombin (Zhang K. et al, 2016 ), mushroom tyrosinase (Das et al, 2015 ), and oxidative reactions (Shin et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

“…On the one hand, Daly et al ( 2016a ) obtain 4, 16, 20, and 36 kPa for alginate, agarose, PEGMA, and GelMA hydrogels, respectively. On the other hand, Aydogdu et al ( 2019 ) perform their tests in the 1,200–1,400 kPa range. They use different mixtures of alginate, collagen, chitosan, Hlh (Halomonas levan), PLA, and b-TCP to create different hydrogels, obtaining 1,240, 1,290, 1,380, 1,490, 1,540, and 1,590 kPa for each hydrogel.…”

Section: Resultsmentioning

confidence: 99%

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Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Sánchez¹,

Gómez-Blanco²,

Nieto

et al. 2020

Front. Bioeng. Biotechnol.

117

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Nowadays, bioprinting is rapidly evolving and hydrogels are a key component for its success. In this sense, synthesis of hydrogels, as well as bioprinting process, and cross-linking of bioinks represent different challenges for the scientific community. A set of unified criteria and a common framework are missing, so multidisciplinary research teams might not efficiently share the advances and limitations of bioprinting. Although multiple combinations of materials and proportions have been used for several applications, it is still unclear the relationship between good printability of hydrogels and better medical/clinical behavior of bioprinted structures. For this reason, a PRISMA methodology was conducted in this review. Thus, 1,774 papers were retrieved from PUBMED, WOS, and SCOPUS databases. After selection, 118 papers were analyzed to extract information about materials, hydrogel synthesis, bioprinting process, and tests performed on bioprinted structures. The aim of this systematic review is to analyze materials used and their influence on the bioprinting parameters that ultimately generate tridimensional structures. Furthermore, a comparison of mechanical and cellular behavior of those bioprinted structures is presented. Finally, some conclusions and recommendations are exposed to improve reproducibility and facilitate a fair comparison of results.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Sánchez¹,

Gómez-Blanco²,

Nieto

et al. 2020

Front. Bioeng. Biotechnol.

117

View full text Add to dashboard Cite

show abstract

“…To date, great efforts have been made to develop suitable bio-inks to provide cell-laden scaffolds with good mechanical properties as well as high cell viability. Hydrogels are often used as supporting material in the bio-ink due to their favorable intrinsic properties for supporting cellular growth [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ]. Their unique inherent properties similar to the extracellular matrix (EMC), such as porosity that allows nutrient and gaseous exchange, high water content, biodegradability, and biocompatibility make them attractive for cell therapy applications [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

3D Printing of a Reactive Hydrogel Bio-Ink Using a Static Mixing Tool

et al. 2020

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Hydrogel-based bio-inks have recently attracted more attention for 3D printing applications in tissue engineering due to their remarkable intrinsic properties, such as a cell supporting environment. However, their usually weak mechanical properties lead to poor printability and low stability of the obtained structures. To obtain good shape fidelity, current approaches based on extrusion printing use high viscosity solutions, which can compromise cell viability. This paper presents a novel bio-printing methodology based on a dual-syringe system with a static mixing tool that allows in situ crosslinking of a two-component hydrogel-based ink in the presence of living cells. The reactive hydrogel system consists of carboxymethyl chitosan (CMCh) and partially oxidized hyaluronic acid (HAox) that undergo fast self-covalent crosslinking via Schiff base formation. This new approach allows us to use low viscosity solutions since in situ gelation provides the appropriate structural integrity to maintain the printed shape. The proposed bio-ink formulation was optimized to match crosslinking kinetics with the printing process and multi-layered 3D bio-printed scaffolds were successfully obtained. Printed scaffolds showed moderate swelling, good biocompatibility with embedded cells, and were mechanically stable after 14 days of the cell culture. We envision that this straightforward, powerful, and generalizable printing approach can be used for a wide range of materials, growth factors, or cell types, to be employed for soft tissue regeneration.

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3‐Dimensional Printing of Hydrogel‐Based Nanocomposites: A Comprehensive Review on the Technology Description, Properties, and Applications

et al. 2021

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Hydrogels are notable biomaterials in bioengineering and biotechnology due to their outstanding biocompatibility, good permeability to water-soluble metabolites, and the high swelling ratio. [1] Hydrogels are extensively used in regenerative medicine and tissue engineering, such as biomarker detection, [2] stem cell research, [3] and organ-on-a-chip (OOC) applications. Hydrogels are chemically or physically cross-linked natural or synthetic threedimensional (3D) networks that can form into various shapes and hold large amounts of water, which could be up to 4000% by dry weight, while they are hardly dissolved in water. [4] Their water retention properties are primarily because of the existence of hydrophilic groups in polymer chains such as amido, amino, carboxyl, and hydroxyl. The degree of swelling depends on the composition of the polymer, the density, and the Increasing demand for customized implants and tissue scaffolds requires advanced biomaterials and fabricating processes for fabricating three-dimensional (3D) structures that resemble the complexity of the extracellular matrix (ECM). Lately, biofabrication approaches such as cell-laden (soft) hydrogel 3D printing (3DP) have been of increasing interest in the development of 3D functional environments similar to natural tissues and organs. Hydrogels that resemble biological ECMs can provide mechanical support and signaling cues to cells to control their behavior. Although the capability of hydrogels to produce artificial ECMs can regulate cellular behavior, one of the major drawbacks of working with hydrogels is their inferior mechanical properties. Therefore, keeping and enhancing the mechanical integrity of fabricated scaffolds has become an essential matter for 3D hydrogel structures. Herein, 3D-printed hydrogel-based nanocomposites (NCs) are evaluated systematically in terms of introducing novel techniques for 3DP of hydrogel-based materials, properties, and biomedical applications.

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Comparative characterization of the hydrogel added PLA/β-TCP scaffolds produced by 3D bioprinting

Cited by 35 publications

References 40 publications

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

3D Printing of a Reactive Hydrogel Bio-Ink Using a Static Mixing Tool

3‐Dimensional Printing of Hydrogel‐Based Nanocomposites: A Comprehensive Review on the Technology Description, Properties, and Applications

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