3D Printing of a Reactive Hydrogel Bio-Ink Using a Static Mixing Tool

Puertas-Bartolomé, María; Włodarczyk‐Biegun, Małgorzata K.; Campo, Aránzazu del; Vázquez, Blanca; Román, Julio San

doi:10.3390/polym12091986

Cited by 40 publications

(35 citation statements)

References 82 publications

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“…Due to the superior self-healing capacity of Schiff base hydrogels, printed structures usually have large primary pores, which makes it difficult to ensure sufficient microstructure. Otherwise, complex methods or additional agents were required to achieve printing [ [60] , [61] , [62] ]. For GEL/PEG-SG system, although the stable amide bonds were destroyed inevitably during printing, the slow gelation of the system continued to occur after destructive deformation ( Fig.…”

Section: Discussionmentioning

confidence: 99%

A structure-supporting, self-healing, and high permeating hydrogel bioink for establishment of diverse homogeneous tissue-like constructs

Chen

Fei

et al. 2021

Bioactive Materials

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The ready-to-use, structure-supporting hydrogel bioink can shorten the time for ink preparation, ensure cell dispersion, and maintain the preset shape/microstructure without additional assistance during printing. Meanwhile, ink with high permeability might facilitate uniform cell growth in biological constructs, which is beneficial to homogeneous tissue repair. Unfortunately, current bioinks are hard to meet these requirements simultaneously in a simple way. Here, based on the fast dynamic crosslinking of aldehyde hyaluronic acid (AHA)/N-carboxymethyl chitosan (CMC) and the slow stable crosslinking of gelatin (GEL)/4-arm poly(ethylene glycol) succinimidyl glutarate (PEG-SG), we present a time-sharing structure-supporting (TSHSP) hydrogel bioink with high permeability, containing 1% AHA, 0.75% CMC, 1% GEL and 0.5% PEG-SG. The TSHSP hydrogel can facilitate printing with proper viscoelastic property and self-healing behavior. By crosslinking with 4% PEG-SG for only 3 min, the integrity of the cell-laden construct can last for 21 days due to the stable internal and external GEL/PEG-SG networks, and cells manifested long-term viability and spreading morphology. Nerve-like, muscle-like, and cartilage-like in vitro constructs exhibited homogeneous cell growth and remarkable biological specificities. This work provides not only a convenient and practical bioink for tissue engineering, targeted cell therapy, but also a new direction for hydrogel bioink development.

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Section: Discussionmentioning

confidence: 99%

A structure-supporting, self-healing, and high permeating hydrogel bioink for establishment of diverse homogeneous tissue-like constructs

Chen

Fei

et al. 2021

Bioactive Materials

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“…The shear force during extrusion contributes to the rearrangement of fibrin fibers. Some static mixers are also adopted to endow hydrogels with internal anisotropic structures [ 167 ]. Besides, scaffolds with aligned micro-channels inside the hydrogel can be achieved directly through 3D printing ( Fig.…”

Section: Anisotropic 3d Hydrogel Scaffoldsmentioning

confidence: 99%

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

Xue

Shi

Kong

et al. 2021

Bioactive Materials

112

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The treatment of long-gap (>10 mm) peripheral nerve injury (PNI) and spinal cord injury (SCI) remains a continuous challenge due to limited native tissue regeneration capabilities. The current clinical strategy of using autografts for PNI suffers from a source shortage, while the pharmacological treatment for SCI presents dissatisfactory results. Tissue engineering, as an alternative, is a promising approach for regenerating peripheral nerves and spinal cords. Through providing a beneficial environment, a scaffold is the primary element in tissue engineering. In particular, scaffolds with anisotropic structures resembling the native extracellular matrix (ECM) can effectively guide neural outgrowth and reconnection. In this review, the anatomy of peripheral nerves and spinal cords, as well as current clinical treatments for PNI and SCI, is first summarized. An overview of the critical components in peripheral nerve and spinal cord tissue engineering and the current status of regeneration approaches are also discussed. Recent advances in the fabrication of anisotropic surface patterns, aligned fibrous substrates, and 3D hydrogel scaffolds, as well as their in vitro and in vivo effects are highlighted. Finally, we summarize potential mechanisms underlying the anisotropic architectures in orienting axonal and glial cell growth, along with their challenges and prospects.

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“…A different approach was presented by Puertas et al in which the carboxymethyl derivative of chitosan was crosslinked with partially oxidized hyaluronic acid via Schiff base formation [155]. This study presented a novel bioprinting methodology based on a dual-syringe system with a static mixing tool that allowed the in situ crosslinking of the reactive hydrogel-based ink in the presence of living cells.…”

Section: Chitosan Based Bioinksmentioning

confidence: 99%

Emerging Biofabrication Techniques: A Review on Natural Polymers for Biomedical Applications

2021

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Natural polymers have been widely used for biomedical applications in recent decades. They offer the advantages of resembling the extracellular matrix of native tissues and retaining biochemical cues and properties necessary to enhance their biocompatibility, so they usually improve the cellular attachment and behavior and avoid immunological reactions. Moreover, they offer a rapid degradability through natural enzymatic or chemical processes. However, natural polymers present poor mechanical strength, which frequently makes the manipulation processes difficult. Recent advances in biofabrication, 3D printing, microfluidics, and cell-electrospinning allow the manufacturing of complex natural polymer matrixes with biophysical and structural properties similar to those of the extracellular matrix. In addition, these techniques offer the possibility of incorporating different cell lines into the fabrication process, a revolutionary strategy broadly explored in recent years to produce cell-laden scaffolds that can better mimic the properties of functional tissues. In this review, the use of 3D printing, microfluidics, and electrospinning approaches has been extensively investigated for the biofabrication of naturally derived polymer scaffolds with encapsulated cells intended for biomedical applications (e.g., cell therapies, bone and dental grafts, cardiovascular or musculoskeletal tissue regeneration, and wound healing).

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3D Printing of a Reactive Hydrogel Bio-Ink Using a Static Mixing Tool

Cited by 40 publications

References 82 publications

A structure-supporting, self-healing, and high permeating hydrogel bioink for establishment of diverse homogeneous tissue-like constructs

A structure-supporting, self-healing, and high permeating hydrogel bioink for establishment of diverse homogeneous tissue-like constructs

Anisotropic scaffolds for peripheral nerve and spinal cord regeneration

Emerging Biofabrication Techniques: A Review on Natural Polymers for Biomedical Applications

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