Comparative analysis of mRNA and protein expression of Popdc1 (Bves) during early development in the chick embryo†

Abstract

The isolation of the Popeye gene family was based on its preferential expression in striated muscle tissue. Recently, a monoclonal antibody against chick Popdc1 (also known as Bves) became available and was used in this study to comparatively analyze the expression pattern of Popdc1 at both the protein and mRNA level during early chick embryogenesis. Using whole-mount immunohistochemistry, expression in the heart was first observed at Hamburger and Hamilton (HH) stage 10 in the presumptive left ventricular segment. Cardiac expression was confined to differentiated cardiac myocytes, and undifferentiated myocytes at the anterior and posterior pole showed little expression. After looping, the outer curvature myocardium showed prominent Popdc1 staining, whereas the inner curvature was unlabeled. Despite previous reports, Popdc1 protein was not detectable at any time point in the proepicardium, epicardium, or the smooth muscle layer of the coronary vessels. Whole-mount in situ hybridization using a full-length Popdc1 probe detected novel expression domains, which have not been described previously. Popdc1 mRNA was found in Hensen's node at HH stage 4, and by HH stage 5؉, expression became asymmetric. In addition, Popdc1 mRNA was found in pharyngeal endoderm and in the notochordal plate. Subsequently, beginning at HH stage 9, Popdc1 mRNA expression was found in the cardiac mesoderm and expression was maintained in the heart in a pattern very similar to the one observed by antibody staining. Developmental Dynamics 235: 691-700, 2006.

Expression pattern of Popdc2 during mouse embryogenesis and in the adult

“…1A). Of interest, expression in Hensen's node has also been previously reported for chick Popdc1 (Torlopp et al, 2006). At E7.5, strong expression in the cardiac crescent was observed; in addition, weaker expression was present in the amnion (Fig.…”

Popeye domain containing proteins are essential for stress-mediated modulation of cardiac pacemaking in mice

“…9; and also referred to as Bves; ref. 8), Popdc2 (previously known as Pop2), and Popdc3 (previously known as Pop3) (8,9,(12)(13)(14)(15). Popdc proteins contain 3 transmembrane helices and the evolutionary conserved cytoplasmic Popeye domain (10,11).…”

Embryologic Association of Tornwaldt's Cyst with Cerebral Artery Abnormalities and Infarction: A Case Report

“…Notochord-derived hedgehog (Hh) is a key molecular cue for the specification and differentiation of multiple tissues and organs during embryonic development. For vasculogenesis/angiogenesis, Hh also plays an important role for appropriate blood island formation in yolk sac vessel development [15], endothelial tube formation [16], and yolk sac angiogenesis in the murine embryo [17] and modulation of angioblastic migration from the posterior lateral mesoderm [18], initiation of artery-specific gene expression [19, 20], and the earliest stages of endocardial specification in zebrafish [21]. Perturbation of the notochord, similar to the events causing the formation of a Tornwaldt's cyst during development, could result in dysfunction of endoderm development or of cell signaling via Hh during vasculogenesis/angiogenesis.…”