Expression pattern of <i>Popdc2</i> during mouse embryogenesis and in the adult

Froese, Alexander; Brand, Thomas

doi:10.1002/dvdy.21431

Cited by 20 publications

(34 citation statements)

References 17 publications

(24 reference statements)

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“…It will also be important to study the phenotype of popdc1 and popdc3 morphants in zebrafish. It is likely that cardiac and skeletal muscle phenotypes are also present in these morphants given that in all vertebrates studied so far, the Popdc genes are expressed in muscle and heart (Andrée et al, 2000; Breher et al, 2004; Froese and Brand, 2008; Hitz et al, 2002; Parnes et al, 2007; Torlopp et al, 2006). …”

Section: Discussionmentioning

confidence: 99%

“…Popdc2 is uniquely present in vertebrates, whereas Popdc1 and Popdc3 orthologs are also found in lower chordates and insects (Brand, 2005; Lin et al, 2007). All three genes in vertebrates display an overlapping and evolutionary conserved expression pattern, being highly abundant in cardiac and skeletal muscle, suggesting an important function in these tissues (Andrée et al, 2000; Breher et al, 2004; Froese and Brand, 2008; Hitz et al, 2002; Parnes et al, 2007; Torlopp et al, 2006). Popdc genes are also expressed in other cell types such as smooth muscle cells, neurons, and several types of epithelial cells, which indicate a more wide-spread role for this gene family (Brand, 2005; Osler et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, protein partners for Popdc1 have been identified, the guanine nucleotide exchange factor T (GEFT), which acts as a GEF for Rho-family GTPases (Smith et al, 2008), and the SNARE proteins VAMP2/VAMP3, which control vesicular transport and β1-integrin recycling (Hager et al, 2010). A null mutation of Popdc2 has recently been engineered in mice (Froese and Brand, 2008) and interestingly, a cardiac arrhythmia phenotype was present in null mutants (Froese et al, 2012). …”

Section: Introductionmentioning

confidence: 99%

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The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development

Kirchmaier

Poon

Schwerte

et al. 2012

Developmental Biology

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The Popeye domain containing (Popdc) genes encode a family of transmembrane proteins with an evolutionary conserved Popeye domain. These genes are abundantly expressed in striated muscle tissue, however their function is not well understood. In this study we have investigated the role of the popdc2 gene in zebrafish. Popdc2 transcripts were detected in the embryonic myocardium and transiently in the craniofacial and tail musculature. Morpholino oligonucleotide-mediated knockdown of popdc2 resulted in aberrant development of skeletal muscle and heart. Muscle segments in the trunk were irregularly shaped and craniofacial muscles were severely reduced or even missing. In the heart, pericardial edema was prevalent in the morphants and heart chambers were elongated and looping was abnormal. These pathologies in muscle and heart were alleviated after reducing the morpholino concentration. However the heart still was abnormal displaying cardiac arrhythmia at later stages of development. Optical recordings of cardiac contractility revealed irregular ventricular contractions with a 2:1, or 3:1 atrial/ventricular conduction ratio, which caused a significant reduction in heart frequency. Recordings of calcium transients with high spatiotemporal resolution using a transgenic calcium indicator line (Tg(cmlc2:gCaMP)s878) and SPIM microscopy confirmed the presence of a severe arrhythmia phenotype. Our results identify popdc2 as a gene important for striated muscle differentiation and cardiac morphogenesis. In addition it is required for the development of the cardiac conduction system.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development

Kirchmaier

Poon

Schwerte

et al. 2012

Developmental Biology

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“…Hypermethylation of the POPDC3 promoter is thus thought to be a potential mechanism by which long-term POPDC3 suppression is maintained in gastric cancer [45]. Interestingly, in the mouse pyloric epithelium, a specific expression of Popdc2 has been observed [58]. However, an association of POPDC2 with gastric cancer such as adenocarcinoma still awaits future investigations.…”

Section: The Roles Of Popdc Proteins In Cell Proliferationmentioning

confidence: 99%

The Role of the Popeye Domain Containing Gene Family in Organ Homeostasis

Amunjela

Swan

Brand

2019

Cells

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The Popeye domain containing (POPDC) gene family consists of POPDC1 (also known as BVES), POPDC2 and POPDC3 and encodes a novel class of cyclic adenosine monophosphate (cAMP) effector proteins. Despite first reports of their isolation and initial characterization at the protein level dating back 20 years, only recently major advances in defining their biological functions and disease association have been made. Loss-of-function experiments in mice and zebrafish established an important role in skeletal muscle regeneration, heart rhythm control and stress signaling. Patients suffering from muscular dystrophy and atrioventricular block were found to carry missense and nonsense mutations in either of the three POPDC genes, which suggests an important function in the control of striated muscle homeostasis. However, POPDC genes are also expressed in a number of epithelial cells and function as tumor suppressor genes involved in the control of epithelial structure, tight junction formation and signaling. Suppression of POPDC genes enhances tumor cell proliferation, migration, invasion and metastasis in a variety of human cancers, thus promoting a malignant phenotype. Moreover, downregulation of POPDC1 and POPDC3 expression in different cancer types has been associated with poor prognosis. However, high POPDC3 expression has also been correlated to poor clinical prognosis in head and neck squamous cell carcinoma, suggesting that POPDC3 potentially plays different roles in the progression of different types of cancer. Interestingly, a gain of POPDC1 function in tumor cells inhibits cell proliferation, migration and invasion thereby reducing malignancy. Furthermore, POPDC proteins have been implicated in the control of cell cycle genes and epidermal growth factor and Wnt signaling. Work in tumor cell lines suggest that cyclic nucleotide binding may also be important in epithelial cells. Thus, POPDC proteins have a prominent role in tissue homeostasis and cellular signaling in both epithelia and striated muscle.

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“…Apart from being expressed in striated muscle cells, Popdc genes are also expressed in smooth muscle tissue lining the gastrointestinal tract, bladder and uterus. Popdc genes are also present in some neurons of the central and peripheral nervous system, as well as in specific types of epithelial cells such as the pyloric epithelium of the stomach [7]. …”

Section: The Popeye-domain-containing Gene Familymentioning

confidence: 99%

The cAMP-binding Popdc proteins have a redundant function in the heart

Brand¹,

Simrick²,

Poon³

et al. 2014

Biochemical Society Transactions

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Popdc (Popeye-domain-containing) genes encode membrane-bound proteins and are abundantly present in cardiac myocytes and in skeletal muscle fibres. Functional analysis of Popdc1 (Bves) and Popdc2 in mice and of popdc2 in zebrafish revealed an overlapping role for proper electrical conduction in the heart and maintaining structural integrity of skeletal muscle. Popdc proteins mediate cAMP signalling and modulate the biological activity of interacting proteins. The two-pore channel TREK-1 interacts with all three Popdc proteins. In Xenopus oocytes, the presence of Popdc proteins causes an enhanced membrane transport leading to an increase in TREK-1 current, which is blocked when cAMP levels are increased. Another important Popdc-interacting protein is caveolin 3, and the loss of Popdc1 affects caveolar size. Thus a family of membrane-bound cAMP-binding proteins has been identified, which modulate the subcellular localization of effector proteins involved in organizing signalling complexes and assuring proper membrane physiology of cardiac myocytes.

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Expression pattern of Popdc2 during mouse embryogenesis and in the adult

Cited by 20 publications

References 17 publications

The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development

The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development

The Role of the Popeye Domain Containing Gene Family in Organ Homeostasis

The cAMP-binding Popdc proteins have a redundant function in the heart

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