Comparative analysis of genetic diversity and incidence of virulence factors and antibiotic resistance among enterococcal populations from raw fruit and vegetable foods, water and soil, and clinical samples

Abriouel, Hikmate; Omar, Nabil Ben; Molinos, Antonio Cobo; López, Rosario Lucas; Grande, María José López; Martínez-Viedma, Pilar; Ortega, Elena; Martı́nez-Cañamero, Magdalena; Gálvez, Antonio

doi:10.1016/j.ijfoodmicro.2007.11.067

Cited by 186 publications

(169 citation statements)

References 48 publications

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“…Vancomycin resistance of nosocomial isolates was more common in Enterococcus faecium carrying the vanA gene, as was also shown by previous studies (Abriouel et al, 2008;Duprè et al, 2003;Papadimitriou-Olivgeris et al, 2014). No enterococcal water isolate was VRE.…”

Section: Discussionsupporting

confidence: 75%

“…They constitute an important cause of nosocomial infections, the most common species being Enterococcus faecalis and Enterococcus faecium (Abriouel et al, 2008;Comerlato et al, 2013;Papadimitriou-Olivgeris et al, 2014). Their ability to produce biofilm has been implicated in the induction of infections (Upadhyaya et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…A high proportion of enterococci from water samples often produce biofilm and carry virulence genes, which have been implicated in the induction of clinical infections. (Abriouel et al, 2008;Ahmad et al, 2014;Comerlato et al, 2013) The presence of such isolates in river or coastal waters poses an important public health problem since they can be a relevant source of resistance and virulence spread to humans.…”

Section: Introductionmentioning

confidence: 99%

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Biofilm synthesis and presence of virulence factors among enterococci isolated from patients and water samples

Papadimitriou-Olivgeris

Filippidou

Δρούγκα

et al. 2015

Journal of Medical Microbiology

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The goal of this study was to compare biofilm synthesis among enterococci recovered from clinical samples (infection or colonization) of patients as well as environmental samples in order to determine possible virulence factors and clonal relationship. During a two-year period, clinical samples (blood, catheter tips, bronchial secretions, wounds, peritoneal fluid, urine) and rectal swabs collected from hospitalized patients as well as environmental water samples were tested for the presence of Enterococcus faecalis and Enterococcus faecium. Antibiotic susceptibility testing was performed by the disc diffusion method and Etest. Strains were tested for the presence of vanA, vanB, esp, ace and asp genes by PCR. Clones were identified by PFGE (Sma I). From infected patients, 48 strains were identified: 24 Enterococcus faecium (10 vanApositive, 14 vancomycin-susceptible) and 24 Enterococcus faecalis (one vanA-positive, 23 vancomycin-susceptible). Among 143 colonizing isolates, 134 were Enterococcus faecium (58 vanA-positive, 11 vanB-positive, 65 vancomycin-susceptible) and nine Enterococcus faecalis (three vanA-positive, two vanB-positive, four vancomycin-susceptible). Among 167 environmental water samples, 51 Enterococcus faecalis and 19 Enterococcus faecium isolates, all glycopeptide-susceptible, were recovered. In total, 64 strains produced biofilm, whereas 34 were esp-positive, 64 asp-positive and 54 ace-positive. Biofilm production was associated with the presence of esp (P,0.001) and ace genes (P50.021), being higher in infecting (P,0.001) and water (P 0.005) isolates as compared with colonizing ones. Clones of environmental waterstrains were different than the patients' clones. The differences found in the incidence of antibiotic resistance, virulence factors and clones suggest that hospital and water enterococci are of different origin.

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Section: Discussionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biofilm synthesis and presence of virulence factors among enterococci isolated from patients and water samples

Papadimitriou-Olivgeris

Filippidou

Δρούγκα

et al. 2015

Journal of Medical Microbiology

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“…Previous studies have reported appreciable numbers of antibiotic resistant members on the consumer-ready vegetables, such as lettuce and spinach leaves [17,46,47]. Root endophytes can be recruited from common soil bacteria and survived in the interior of the root [48,49], which allow ARGs transferring from soil into plant.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic resistance genes in manure-amended soil and vegetables at harvest

Wang

Qiao

Chen

et al. 2015

Journal of Hazardous Materials

280

110

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“…The micro-organisms that are normally found in these regions are competing to develop more resistant micro-organisms. The elimination of the normal flora of the indiscriminate use of antibiotics has resulted in the development of multiresistant pathogenic microorganisms [29].…”

Section: Journal Of Healthcare Communications Issn 2472-1654mentioning

confidence: 99%