Biofilm synthesis and presence of virulence factors among enterococci isolated from patients and water samples

Papadimitriou-Olivgeris, Matthaios; Filippidou, Sevasti; Δρούγκα, Ελεάννα; Fligou, Fotini; Kolonitsiou, Fevronia; Dodou, Vasiliki; Marangos, Markos; Anastassiou, Evangelos D.; Vantarakis, Apostolos; Spiliopoulou, Iris

doi:10.1099/jmm.0.000151

Cited by 10 publications

(4 citation statements)

References 28 publications

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“…striatum strains may have evolved the ability to form robust biofilms under specific circumstances. This hypothesis might be supported by previous studies demonstrating that the increased level of biofilm formation was associated with the infection status and not strain clonality in Staphylococcus aureus and Enterococcal disease [ 36 , 37 ]. In contrast to our finding, Souza et al .…”

Section: Discussionsupporting

confidence: 61%

Factors affecting the clinical relevance of Corynebacterium striatum isolated from blood cultures

Kang¹,

Choi²,

Choi³

et al. 2018

PLoS ONE

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This study aimed to identify clinical or microbiological factors affecting the clinical relevance of Corynebacterium striatum isolated from blood cultures. A total of 64 isolates from 51 patients identified as C. striatum by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing were assessed. More than two blood cultures were positive in 25 (48.1%) patients. Diabetes, solid tumor, and a history of previous exposure to antibiotics were more common in patients with multiple positive blood cultures. Charlson comorbidity scores were also higher, and more isolates were recovered after 48 hours of hospital stay in patients with multiple positive blood cultures. Strains recovered from patients with multiple positive blood cultures produced significantly more biofilm. Based on multilocus sequence typing (MLST), sequence type (ST) 20 (31.3%) was the most dominant, followed by ST2 (20.3%) and ST23 (10.9%). There was no relationship between the number of positive blood culture sets and sequence typing. In multivariate analyses, Carlson comorbidity score (odds ratio [OR], 1.91; 95% confidence interval [CI], 1.09–3.36; P = 0.03) and biofilm formation were associated with multiple positive blood cultures (OR, 17.43; 95% CI, 3.71–81.91; P = 0.03). This study provides evidence that the biofilm phenotype could contribute to determining the clinical significance of C. striatum in patients with severe underlying conditions. The predominance of certain STs suggests the relatedness of C. striatum infection and the nosocomial environment.

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Section: Discussionsupporting

confidence: 61%

Factors affecting the clinical relevance of Corynebacterium striatum isolated from blood cultures

Kang¹,

Choi²,

Choi³

et al. 2018

PLoS ONE

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“…Using 0.5% seemed reasonable. Likewise, we consistently performed the assays after 48 hours incubation based on prior reports 28, 29 ; this consistency was important to guarantee that the incubation time would not be a confounding factor.…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation in enterococci: genotype-phenotype correlations and inhibition by vancomycin

Hashem

Amin

Essam

et al. 2017

Sci Rep

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Enterococci are nosocomial pathogens that can form biofilms, which contribute to their virulence and antibiotic resistance. Although many genes involved in biofilm formation have been defined, their distribution among enterococci has not been comprehensively studied on a genome scale, and their diagnostic ability to predict biofilm phenotypes is not fully established. Here, we assessed the biofilm-forming ability of 90 enterococcal clinical isolates. Major patterns of virulence gene distribution in enterococcal genomes were identified, and the differentiating virulence genes were screened by polymerase chain reaction (PCR) in 31 of the clinical isolates. We found that detection of gelE in Enterococcus faecalis is not sufficient to predict gelatinase activity unless fsrAB, or fsrB alone, is PCR-positive (P = 0.0026 and 0.0012, respectively). We also found that agg is significantly enriched in isolates with medium and strong biofilm formation ability (P = 0.0026). Additionally, vancomycin, applied at sub minimal inhibitory concentrations, inhibited biofilm in four out of five strong biofilm-forming isolates. In conclusion, we suggest using agg and fsrB genes, together with the previously established gelE, for better prediction of biofilm strength and gelatinase activity, respectively. Future studies should explore the mechanism of biofilm inhibition by vancomycin and its possible use for antivirulence therapy.

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“…The esp gene was de ned as characteristic to hospital strains and claimed to be greatly correlated to the capability of the isolates to cause health-care associated infection [36]. The importance of the presence of esp and ace genes in the bio lm synthesis process was highlighted by the results of a study conducted by Papadimitriou, et al, 2015 [37]. In the present study, 97.7% of the isolates had esp gene, while 17.7% of them were contained the ace gene.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Virulence Determination of Enterococcus spp. Obtained from Hospital Environment in Iran

Marghmalek

Valadan

Gholami

et al. 2020

Preprint

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Background: The role of the hospital environment as a source of pathogenic bacteria in recent studies has been poorly investigated. This study investigated the distribution of antimicrobial resistance genes and virulence determinants in Enterococcus species isolated from hospital environment in Sari, Iran. Method: Overall, 90 enterococci strains were obtained from high touch surfaces of four hospitals in Sari, Iran. These environmental samples were obtained from bathroom, beds, tables, doorknobs, room keys, wheelchair and walls in the patient and staff’s rooms. The resistance profile of the isolates was determined by disk diffusion method. Seven resistance genes and two virulence associated genes were evaluated molecularly by multiplex PCR. Results: According to the PCR, 42 (46.66%) of them were E. faecalis and 48 (53.33%) others were detected as E. faecium. Also, 28 (66.6%) E. faecalis and 18 (37.5%) E. faecium isolates were multidrug-resistant (MDR). Among all 90 environmental isolates 54 (60%), 54 (60%), 8 (8.8%), 8 (8.8%), 60 (66.6%), 26 (28.8%), and 24 (26.6%) isolates contained tetM, tetL, vanA, vanB, ermB, aac(6´)-Ie-aph(2´´)-Ia, and aph (3´)-IIIa, respectively. Moreover, all isolates were investigated for the presence of virulence genes and 88 (97.7%) of isolates had esp gene, and 16 (17.7%) had ace.Conclusions: This report showed that the environmental isolates of Enterococcus are the major sources of antibiotic resistance genes that can transfer them to the clinical isolates of bacteria in hospital settings. An effective following strategy should be organized to clearance and stop emergence of these pathogenic bacteria.

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Biofilm synthesis and presence of virulence factors among enterococci isolated from patients and water samples

Cited by 10 publications

References 28 publications

Factors affecting the clinical relevance of Corynebacterium striatum isolated from blood cultures

Factors affecting the clinical relevance of Corynebacterium striatum isolated from blood cultures

Biofilm formation in enterococci: genotype-phenotype correlations and inhibition by vancomycin

Antimicrobial Resistance and Virulence Determination of Enterococcus spp. Obtained from Hospital Environment in Iran

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