Antibiotic resistance genes in manure-amended soil and vegetables at harvest

Wang, Fenghua; Qiao, Mingqiang; Chen, Zheng; Su, Jian‐Qiang; Zhu, Yan

doi:10.1016/j.jhazmat.2015.05.028

Cited by 280 publications

(133 citation statements)

References 54 publications

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“…Widespread manure distribution, either through feedlots or land spreading can facilitate the dissemination of ARGs (Aust et al, 2008; McEachran et al, 2015; Wang et al, 2015). In addition, several antibiotics have been recorded downwind of feedlots at concentrations similar to that found in rivers downstream of sewage outlets (0.5 to 4.6 μg/g).…”

Section: Relevance Of Amr To Environmental Regulators?mentioning

confidence: 99%

Review of Antimicrobial Resistance in the Environment and Its Relevance to Environmental Regulators

et al. 2016

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The environment is increasingly being recognized for the role it might play in the global spread of clinically relevant antibiotic resistance. Environmental regulators monitor and control many of the pathways responsible for the release of resistance-driving chemicals into the environment (e.g., antimicrobials, metals, and biocides). Hence, environmental regulators should be contributing significantly to the development of global and national antimicrobial resistance (AMR) action plans. It is argued that the lack of environment-facing mitigation actions included in existing AMR action plans is likely a function of our poor fundamental understanding of many of the key issues. Here, we aim to present the problem with AMR in the environment through the lens of an environmental regulator, using the Environment Agency (England’s regulator) as an example from which parallels can be drawn globally. The issues that are pertinent to environmental regulators are drawn out to answer: What are the drivers and pathways of AMR? How do these relate to the normal work, powers and duties of environmental regulators? What are the knowledge gaps that hinder the delivery of environmental protection from AMR? We offer several thought experiments for how different mitigation strategies might proceed. We conclude that: (1) AMR Action Plans do not tackle all the potentially relevant pathways and drivers of AMR in the environment; and (2) AMR Action Plans are deficient partly because the science to inform policy is lacking and this needs to be addressed.

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Section: Relevance Of Amr To Environmental Regulators?mentioning

confidence: 99%

Review of Antimicrobial Resistance in the Environment and Its Relevance to Environmental Regulators

et al. 2016

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“…Thus, there is a need to better understand the factors that affect the dissemination of ARGs and associated mobile genetic elements in soil bacterial populations when soils are subject to application of manure and compost, particularly when they contain antibiotics (Aminov, 2011; Heuer et al, 2011). The potential for antibiotics, antibiotic‐resistant bacteria, and ARGs to enter the food chain is of special interest (Wang et al, 2015), as amending soils with manure has been observed to influence ARGs associated with plants, including vegetables and fruits (Chen et al, 2017).…”

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confidence: 99%

Antibiotics and Antibiotic Resistance Genes in Bulk and Rhizosphere Soils Subject to Manure Amendment and Vegetable Cultivation

et al. 2018

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There is interest in understanding effects of amending soil with manure in a cultivation setting and if composting can provide benefits. Raw or composted manure from cattle administered with and without sulfamethazine, chlortetracycline, and tylosin was amended to loamy sand and silty clay loam soils, where lettuce (Lactuca sativa L.), radish (Raphanus sativus L.), and broccoli (Brassica oleracea L. var. italica) were cultivated and compared with those grown in soil amended with fertilizer as a control. Upon plant maturation, rhizosphere and bulk soils were analyzed for antibiotics, and sul1, ermB, tet(W), and intI1 genes were quantified. Antibiotic concentrations in compost‐amended soils were below detection limits. For soils amended with manure containing antibiotics, sulfamethazine ranged from 1.1 to 3.1 μg kg−1 in the bulk soils but was below detection limits in the rhizosphere soils. Chlortetracycline (2.8–9.3 μg kg−1) was two times lower in the rhizosphere than in the bulk soil. Levels of tylosin in the rhizosphere soil were similar to the bulk soil. Soil texture or vegetable type did not have significant influence on antibiotic concentration differences between the bulk and rhizosphere soils. Relative abundances of tet(W) and intI1 in the fertilizer‐amended soil were significantly lower than in those amended with manure or compost (p < 0.05), whereas ermB was not detected in any soils. Rhizosphere zone has no significant effect on the detected antibiotic resistance genes. It is suggested that plant roots may have a substantial effect on the fate of certain antibiotics in manure‐amended fields, but less of an effect on antibiotic resistance and mobility genes. Core Ideas Composting reduces antibiotic inputs to soils relative to direct manure application. Antibiotic concentrations in manure generally decrease in amended soils. Dissipation of sulfamethazine and chlortetracycline is enhanced in the rhizosphere. Antibiotic fate in bulk and rhizosphere soils was similar for three vegetables and two soils. Resistance and mobility genes were greater in manure ‐ than fertilizer‐amended soil.

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“…Anthropogenic activities such as RWI and other agricultural practices may contribute to expansion of soil antibiotic resistance (Munir and Xagoraraki, 2011;Cytryn, 2013), and potentially threaten human health through transfer of ARGs and ARB into human-associated pathogens (Forsberg et al, 2012) and food chain (Marti et al, 2013;Wang et al, 2015a). In this study, the numbers and fold changes of the detected ARGs were found to be significantly greater in urban parks with RWI than those without RWI, suggesting that RWI could enhance the diversity and enrichment of soil ARGs.…”

Section: Impacts Of Rwi On the Patterns Of Args In Urban Park Soilsmentioning

confidence: 59%

Impacts of reclaimed water irrigation on soil antibiotic resistome in urban parks of Victoria, Australia

Han

Shi

et al. 2016

Environmental Pollution

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a b s t r a c tThe effluents from wastewater treatment plants have been recognized as a significant environmental reservoir of antibiotics and antibiotic resistance genes (ARGs). Reclaimed water irrigation (RWI) is increasingly used as a practical solution for combating water scarcity in arid and semiarid regions, however, impacts of RWI on the patterns of ARGs and the soil bacterial community remain unclear. Here, we used high-throughput quantitative PCR and terminal restriction fragment length polymorphism techniques to compare the diversity, abundance and composition of a broad-spectrum of ARGs and total bacteria in 12 urban parks with and without RWI in Victoria, Australia. A total of 40 unique ARGs were detected across all park soils, with genes conferring resistance to b-lactam being the most prevalent ARG type. The total numbers and the fold changes of the detected ARGs were significantly increased by RWI, and marked shifts in ARG patterns were also observed in urban parks with RWI compared to those without RWI. The changes in ARG patterns were paralleled by a significant effect of RWI on the bacterial community structure and a co-occurrence pattern of the detected ARG types. There were significant and positive correlations between the fold changes of the integrase intI1 gene and two b-lactam resistance genes (KPC and IMP-2 groups), but no significant impacts of RWI on the abundances of intI1 and the transposase tnpA gene were found, indicating that RWI did not improve the potential for horizontal gene transfer of soil ARGs. Taken together, our findings suggested that irrigation of urban parks with reclaimed water could influence the abundance, diversity, and compositions of a wide variety of soil ARGs of clinical relevance. One-sentence summary: Irrigation of urban parks with treated wastewater significantly increased the abundance and diversity of various antibiotic resistance genes, but did not significantly enhance their potential for horizontal gene transfer.

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Antibiotic resistance genes in manure-amended soil and vegetables at harvest

Cited by 280 publications

References 54 publications

Review of Antimicrobial Resistance in the Environment and Its Relevance to Environmental Regulators

Review of Antimicrobial Resistance in the Environment and Its Relevance to Environmental Regulators

Antibiotics and Antibiotic Resistance Genes in Bulk and Rhizosphere Soils Subject to Manure Amendment and Vegetable Cultivation

Impacts of reclaimed water irrigation on soil antibiotic resistome in urban parks of Victoria, Australia

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