Comparative analysis of different collagen-based biomaterials as scaffolds for long-term culture of human fibroblasts

Vaissiere, G.; Chevallay, B.; Herbage, D.; Damour, O.

doi:10.1007/bf02344778

Cited by 55 publications

(37 citation statements)

References 26 publications

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“…Collagen constitutes the greatest quantity of total proteins in human body, and is the major composition of extracellular matrix. Collagen gels as scaffolds have already been used successfully in the tissue engineering of skin because of their excellent biocompatibility, low antigenicity and high biodegradability (Jimenez and Jimenez 2004;Jones et al 2002;Auger et al 2004;Lee et al 2001;Vaissiere et al 2000). However, one potential drawback of using collagen gels in tissue-engineering is their dramatic contraction after being mixed with cells.…”

Section: Introductionmentioning

confidence: 97%

Compressed collagen gel as the scaffold for skin engineering

Shi

Zhu

et al. 2010

Biomed Microdevices

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Collagen gel scaffolds can potentially be utilized as cell seeded systems for skin tissue engineering. However, its dramatic contraction after being mixed with cells and its mechanical weakness are the drawbacks for its application to skin engineering. In this study, a compressed collagen gel scaffold was fabricated through the rapid expulsion of liquid from reconstituted gels by the application of 'plastic compression'(PC) technique. Both compressed and uncompressed gels were characterized with their gel contraction rate, morphology, the viability of seeded cells, their mechanical properties and the feasibility as a scaffold for constructing tissue-engineered skin. The results showed that the compression could significantly reduce the contraction of the collagen gel and improve its mechanical property. In addition, seeded dermal fibroblasts survived well in the compressed gel and seeded epidermal cells gradually developed into a stratified epidermal layer, and thus formed tissue engineered skin. This study reveals the potential of using compressed collagen gel as a scaffold for skin engineering.

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Section: Introductionmentioning

confidence: 97%

Compressed collagen gel as the scaffold for skin engineering

Shi

Zhu

et al. 2010

Biomed Microdevices

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“…The disulfide-functionalized diblock copolymer hydrogel described herein is expected to be preferable to commercial protein-based gels, particularly for applications where the biological effects of such animal-derived gels are not acceptable, or are too variable. [27] This new synthetic hydrogel permits 3D culture of cells supported in mesh sheets and can be used to evaluate the effects of cell-ECM proteins for at least 12 days. In principle, thiol-disulfide chemistry can be used for convenient hydrogel functionalization with RGD, DNA or adhesion proteins to evaluate how these biomolecules influence cellular growth and proliferation.…”

Section: Cellular Densities Of A549-gfp Cells Became Indistinguishablmentioning

confidence: 99%

Disulfide-Based Diblock Copolymer Worm Gels: A Wholly-Synthetic Thermoreversible 3D Matrix for Sheet-Based Cultures

et al. 2015

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It is well-known that 3D in vitro cell cultures provide a much better model than 2D cell cultures for understanding the in vivo microenvironment of cells. However, significant technical challenges in handling and analyzing 3D cell cultures remain, which currently limits their widespread application. Herein, we demonstrate the application of wholly synthetic thermoresponsive block copolymer worms in sheet-based 3D cell culture. These worms form a soft, free-standing gel reversibly at 20–37 °C, which can be rapidly converted into a free-flowing dispersion of spheres on cooling to 5 °C. Functionalization of the worms with disulfide groups was found to be essential for ensuring sufficient mechanical stability of these hydrogels to enable long-term cell culture. These disulfide groups are conveniently introduced via statistical copolymerization of a disulfide-based dimethacrylate under conditions that favor intramolecular cyclization and subsequent thiol/disulfide exchange leads to the formation of reversible covalent bonds between adjacent worms within the gel. This new approach enables cells to be embedded within micrometer-thick slabs of gel with good viability, permits cell culture for at least 12 days, and facilitates recovery of viable cells from the gel simply by incubating the culture in buffer at 4 °C (thus, avoiding the enzymatic degradation required for cell harvesting when using commercial protein-based gels, such as Matrigel).

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“…Unfortunately, collagen sponges have a weak resistance at physiological temperature as they denaturate and contract. For this reason, sponges need to be cross-linked by chemical reagents such as glutaraldehyde, carbodiimides, or diphenyphosphorylazide [75,76]. Because of their process of fabrication, collagen sponges are acellular biomaterials.…”

Section: Collagen Spongesmentioning

confidence: 99%

“…Therefore, cells have to migrate and colonize the sponge. After three weeks in culture, fibroblasts seeded at the surface migrate into the sponge, proliferate, and fill the pores by deposition of new ECM components [76]. To fabricate sponges, atelocollagen collagen I can be associated with other molecules (collagen III, fibronectin, glycosaminoglycans) to form a hydrid scaffold [77].…”

Section: Collagen Spongesmentioning

confidence: 99%