Comparative Analyses Between the Smoking Habit Frequency and the Nucleolar Organizer Region Associated Proteins in Exfoliative Cytology of Smokers' Normal Buccal Mucosa

Cançado, Renata Pittella; Yurgel, Liliane; Filho, Manoel da Silva

doi:10.1186/1617-9625-2-4

Cited by 6 publications

(6 citation statements)

References 29 publications

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“…Notwithstanding, the latter has been shown to be more reliable, which justifies its use in the present study. 39 In contrast, Cançado et al 51 found a dose-response relationship between number of cigarettes per day and cell proliferation rate in normal-appearing mucosa of the floor of the mouth.…”

Section: Discussionmentioning

confidence: 93%

Nuclear changes in oral mucosa of alcoholics and crack cocaine users

et al. 2015

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The effects of drugs of abuse on oral mucosa are only partly understood. The aims of the present study were to: (1) evaluate the frequency of nuclear changes in normal-appearing oral mucosa of alcoholics and crack cocaine users and (2) assess their association with cell proliferation rate. Oral smears were obtained from the border of the tongue and floor of the mouth of 26 crack cocaine users (24 males and 2 females), 29 alcoholics (17 males and 12 females), and 35 controls (17 males and 18 females). Histological slides were submitted to Feulgen staining to assess the frequency of micronuclei (MN), binucleated cells (BN), broken eggs (BE), and karyorrhexis (KR). A significant increase in the frequency of MN was observed in cells exfoliated from the tongue of crack cocaine users ( p = 0.01), and alcoholics showed a higher frequency of KR in cells obtained from the floor of the mouth ( p = 0.01). Our findings suggest that the use of crack cocaine induces clastogenic effects, whereas alcoholism is associated with higher degrees of keratinization in the floor of the mouth.

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Section: Discussionmentioning

confidence: 93%

Nuclear changes in oral mucosa of alcoholics and crack cocaine users

et al. 2015

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“…The diagnosis of oral cancers is still difficult and almost half of the individuals diagnosed with oral cancers die. Undoubtedly, the first step in the treatment is early diagnosis, especially in high-risk subjects (Cançado et al, 2004;Sargolzaei et al, 2014). Tobacco smoke and use of hookah are the most important etiologic factors for oral cancers and risk factors for dysplastic lesions.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Hookah Use on Buccal Mucosa: Evaluation of Repair Index

Taghibakhsh

Farhadi

Babaee³

et al. 2019

Asian Pac J Cancer Prev

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Background and aim: Cigarettes, hookah, and tobacco are the most important etiologic factors for oral cancers and dysplastic lesions. This study was undertaken to determine the correlation between hookah use and the percentage of cells with micronucleus, karyorrhexis, karyolysis, and broken egg in the buccal mucosa; and secondly to compare hookah user and non-user in terms of repair index. Materials and methods: The present historical cohort study was carried out on 72 samples taken from 36 hookah users and 36 control subjects. Smear samples were obtained from participants' buccal mucosa for cytological evaluation using Papanicolaou technique. Then, the percentages of cells with micronucleus, karyorrhexis, karyolysis, and broken egg were recorded and the repair index was calculated. Data were analyzed using Mann-Whitney U test. Results: A total of 72 samples taken from 36 hookah users and 36 control subjects were evaluated. The means of micronucleus scores in the buccal mucosa cells of hookah users and controls were 10.7±2.6 and 5.8±2.0, the karyorrhexis scores in the hookah users and controls were 0.1±0.06 and 0.04±0.06, and the karyolysis scores in hookah users and controls were 0.16±0.05 and 0.08±0.06, respectively. These differences were statistically significant between hookah users and controls (P<0.001). The broken egg score was 0.66±0.07 for the hookah users and 0.03±0.04 for the control group, revealing a statistically significant difference (P<0.036). Finally, the repair index values were 0.03±0.01 and 0.05±0.13 in hookah users and controls, respectively. This difference was also significant (P<0.026). Conclusion: The percentages of cells with micronucleus, karyorrhexis, karyolysis, and broken egg in the buccal mucosa of hookah users were significantly higher than those in control group; in addition, the repair index of the buccal mucosa cells in hookah users was significantly lower than that in the control group.

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“…Its quantification occurs when these are active . Studies found that it was increased of AgNORs in normal oral mucosa of individuals exposed to carcinogens, tobacco and alcohol, compared with the control group …”

Section: Introductionmentioning

confidence: 99%

Epithelial oral mucosal cells: Do they behave differently when exposed to oral carcinogens?

et al. 2017

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Cytopathology combined with quantitative techniques such as Papanicolaou, AgNOR, and immunocytochemical expression of E-cadherin detects changes associated with oral carcinogenesis. The innovative approach used in this study allows assessing the expression of cell adhesion (E-cadherin) and differentiation (involucrin) markers by means of oral mucosal cytopathology. The E-cadherin imunocytochemical expression indicated changes associated with the oral carcinogenesis process. An increase in cell proliferation rate in oral squamous cell carcinoma group was associated with the lower immunoexpression of E-cadherin. Cytopathology combined with quantitative techniques and immunocytochemical expression of E-cadherin may detect early alterations associated with oral carcinogenesis.

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Comparative Analyses Between the Smoking Habit Frequency and the Nucleolar Organizer Region Associated Proteins in Exfoliative Cytology of Smokers' Normal Buccal Mucosa

Cited by 6 publications

References 29 publications

Nuclear changes in oral mucosa of alcoholics and crack cocaine users

Nuclear changes in oral mucosa of alcoholics and crack cocaine users

The Effect of Hookah Use on Buccal Mucosa: Evaluation of Repair Index

Epithelial oral mucosal cells: Do they behave differently when exposed to oral carcinogens?

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