Nuclear changes in oral mucosa of alcoholics and crack cocaine users

Webber, Liana Preto; Pellicioli, Ana Carolina Amorim; Magnusson, Alessandra Selinger; Danilevicz, Chris Krebs; Bueno, C.; Filho, Manoel Sant’Ana; Rados, Pantelis Varvaki; Carrard, Vinícius Coelho

doi:10.1177/0960327115579430

Cited by 28 publications

(26 citation statements)

References 48 publications

(93 reference statements)

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“…The results showed that the frequency of micronuclei and karyorrhexis increased significantly. Our results corroborate the findings of some authors, such as Webber et al and de Freitas et al 18,25 These authors observed a frequency of micronuclei and nuclear buds was significantly increased in peripheral blood lymphocytes and oral mucosa. In 2014, das Graças Alonso de Oliveira et al 26 have investigated the frequency of micronucleus in oral mucosa of crack cocaine users too.…”

Section: Discussionsupporting

confidence: 93%

“…It can be repeated several times for the prevention and monitoring of individuals at risk for cancer, such as: chronic users of alcohol, smoking and / or other mutagenic substances. [15][16][17][18] The presence of micronuclei in exfoliated cells of oral mucosa reflects genotoxic events that occurred in cells that were in the basal layer of the epithelium 1-3 weeks before getting the smear. 13 Until the present date, there are few reports in the literature on the possible genotoxic and cytotoxic effects of crack that is often used with other drugs, such as cigarettes and alcohol (carcinogens).…”

Section: -14mentioning

confidence: 99%

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Genotoxic and Cytotoxic Potential of Smoke Crack Cocaine on the Epithelium of the Human Oral Mucosa

et al. 2016

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Crack cocaine is an illicit drug derived from cocaine. It can produce some damages to the lungs and oral cavity. Objective: The aim of this study was to analyze the frequency of micronuclei and some nuclear alterations in epithelial cells of crack cocaine users. Methods: Oral smears were collected from clinically normal-appearing buccal mucosa exfoliative cytology of 30 individuals (15 crack cocaine users and 15 controls). Results: Crack cocaine users consumed about 3.8 grams per day and the time consumption of the drug was of 6.4 +3.3 years. The prevalence of micronuclei, binucleated cells, broken egg cells, budding cells, picnosis, karyolysis, and karyorrhexis was determined. The frequencies of micronuclei for case and control groups were, 2.87+3.46 and 0.57+1.6 (p=0.018) respectively. No statistical difference was observed for binucleated cells, broken egg cells, budding cells, picnosis, and karyolysis. The frequency of karyorrhexis was significantly increased on crack cocaine users than controls (54.07+38.58 and 24.87+23.97, p=0.001). Conclusion: Smoke crack might have a cytotoxic and genotoxic effects to the oral mucosa due to increased frequency of micronuclei and karyorrhexis. Thus, individuals who used crack cocaine in the long term need to be frequently examined in order to prevent neoplastic transformation.

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Section: Discussionsupporting

confidence: 93%

Section: -14mentioning

confidence: 99%

Genotoxic and Cytotoxic Potential of Smoke Crack Cocaine on the Epithelium of the Human Oral Mucosa

et al. 2016

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“…With the introduction of quantitative techniques, oral cytology has become an important preventive exam for monitoring the oral mucosa exposed to carcinogens [2][3][4][5][6] . From these studies, it became apparent the individual nature of this type of evaluation, suggesting that some individuals are more susceptible than others to oral cancer development.…”

Section: Introductionmentioning

confidence: 99%

Influence of factors in the oral mucosa maturation pattern: a cross-sectional study applying multivariate analyses

Baumgart¹,

Daroit²,

Maraschin³

et al. 2016

Braz. J. Oral Sci.

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Aim: To evaluate the association between oral health status, socio-demographic and behavioral factors with the pattern of maturity of normal epithelial oral mucosa. Methods: Exfoliative cytology specimens were collected from 117 men from the border of the tongue and floor of the mouth on opposite sides. Cells were stained with the Papanicolaou method and classified into: anucleated, superficial cells with nuclei, intermediate and parabasal cells. Quantification was made by selecting the first 100 cells in each glass slide. Sociodemographic and behavioral variables were collected from a structured questionnaire. Oral health was analyzed by clinical examination, recording decayed, missing and filled teeth index (DMFT) and use of prostheses. Multivariable linear regression models were applied. Results: No significant differences for all studied variables influenced the pattern of maturation of the oral mucosa except for alcohol consumption. There was an increase of cell surface layers of the epithelium with the chronic use of alcohol. Conclusions: It is appropriate to use Papanicolaou cytopathological technique to analyze the maturation pattern of exposed subjects, with a strong recommendation for those who use alcohol -a risk factor for oral cancer, in which a change in the proportion of cell types is easily detected.

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“…Both methods of sample collection are inexpensive, facile, and non-invasive. [19][20][21] Hence, salivary nitric oxide and the comet assay of buccal cells optimally met all of the above requirements.…”

Section: Figurementioning

confidence: 95%

Status of Salivary Nitric Oxide Levels and Buccal Epithelial Cell DNA Damage in Potentially Malignant Disorders – A Biochemical Study

Ka¹,

Castelino²,

Babu³

et al. 2017

J Dent Indones

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Oral cancer is a progressive, multistage disease in which changes in genetic structure and cellular morphology occur from the normal to the premalignant state and then to the malignant state. Nitric oxide (NO . ) is an uncharged molecule with an unpaired electron. It is highly reactive and interacts with DNA molecules, resulting in DNA damage. Objective: To evaluate the salivary nitric oxide levels and buccal epithelial cell DNA damage in patients with potentially malignant oral disorders. Methods: The salivary nitric oxide levels and buccal epithelial cell DNA damage were estimated in 20 healthy individuals without oral lesions, in 20 subjects having smoking and/or tobacco chewing habits without oral lesions, and 20 patients with a potentially malignant oral disorder. Results: The salivary nitric oxide levels were significantly greater in the subjects with tobacco chewing and/or smoking habits without oral lesions than in the healthy controls. Similarly, the extent of DNA damage was higher in the subjects with potentially malignant disorders and in the subjects with tobacco chewing and/or smoking habits without oral lesions than in the healthy controls. Conclusion: The encouraging results of the present study indicated the potential involvement of nitric oxide in the pathogenesis of potentially malignant oral disorders.

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Nuclear changes in oral mucosa of alcoholics and crack cocaine users

Cited by 28 publications

References 48 publications

Genotoxic and Cytotoxic Potential of Smoke Crack Cocaine on the Epithelium of the Human Oral Mucosa

Genotoxic and Cytotoxic Potential of Smoke Crack Cocaine on the Epithelium of the Human Oral Mucosa

Influence of factors in the oral mucosa maturation pattern: a cross-sectional study applying multivariate analyses

Status of Salivary Nitric Oxide Levels and Buccal Epithelial Cell DNA Damage in Potentially Malignant Disorders – A Biochemical Study

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