Comparación y utilidad de las regiones mitocondriales de los genes 16S y COX1 para los análisis genéticos en garrapatas (Acari: Ixodidae)

Paternina, Luis E.; Verbel‐Vergara, Daniel; Bejarano, Eduar Elías

doi:10.7705/biomedica.v36i2.3116

Cited by 9 publications

(6 citation statements)

References 66 publications

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“…We suggest the utility of rrnL rRNA gene as a useful genetic marker for Trichuris species discrimination. Different authors who evaluated the utility of different mitochondrial genes for phylogenetic analyses (64,(79)(80)(81) have cited similar results. Future studies should focus on developing sequence databases for rRNA genes, which can serve as alternative genetic.…”

Section: Discussionmentioning

confidence: 67%

Trichuris trichiura (Linnaeus, 1771) From Human and Non-human Primates: Morphology, Biometry, Host Specificity, Molecular Characterization, and Phylogeny

2021

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Human trichuriasis is a Neglected Tropical Disease, which affects hundreds of millions of persons worldwide. Several studies have reported that non-human primates (NHP) represent important reservoirs for several known zoonotic infectious diseases. In this context, Trichuris infections have been found in a range of NHP species living in natural habitats, including colobus monkeys, macaques, baboons, and chimpanzees. To date, the systematics of the genus Trichuris parasitizing humans and NHP is unclear. During many years, Trichuris trichiura was considered as the whipworm present in humans and primates. Subsequently, molecular studies suggested that Trichuris spp. in humans and NHP represent several species that differ in host specificity. This work examines the current knowledge of T. trichiura and its relationship to whipworm parasites in other primate host species. A phylogenetic hypothesis, based on three mitochondrial genes (cytochrome c oxidase subunit 1, cytochrome b, and large subunit rRNA-encoding gene) and two fragments of ribosomal DNA (Internal Transcribed Spacer 1 and 2), allowed us to define a complex of populations of T. trichiura hosting in a large variety of NHP species, in addition to humans. These populations were divided into four phylogenetic groups with a different degree of host specificity. From these data, we carry out a new morphological and biometrical description of the populations of Trichuris based on data cited by other authors as well as those provided in this study. The presence of T. trichiura is analyzed in several NHP species in captivity from different garden zoos as possible reservoir of trichuriasis for humans. This study contributes to clarify questions that lead to identification of new taxa and will determine parasite transmission routes between these primates, allowing the implementation of appropriate control and prevention measures.

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Section: Discussionmentioning

confidence: 67%

Trichuris trichiura (Linnaeus, 1771) From Human and Non-human Primates: Morphology, Biometry, Host Specificity, Molecular Characterization, and Phylogeny

2021

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“…Cox1 gene appears to be more useful for intraspecific genetic variability studies (Filipe et al, 2021;Paternina et al, 2016). This highlights the difficulty of using these two genes and the need for more genetic characterization using whole-genome sequencing (Burger et al, 2014;Low et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…microplus clade C. Hence, the morphological classification obtained may not always reflect the molecular results (Nava et al, 2009). In this study where we have used both Cox1 and 16S rDNA as well as morphologic identification keys for the taxonomic assignation of ticks, the species was determined by an identical outcome with at least two of the three tools used as shown elsewhere (Filipe et al, 2021; Paternina et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…microplus showing that 16S rDNA gene is more conserved. It has been shown elsewhere that 16S rDNA gene seems to have better features for interspecific phylogenetic analyses while Cox1 gene appears to be more useful for intraspecific genetic variability studies (Filipe et al, 2021; Paternina et al, 2016). This highlights the difficulty of using these two genes and the need for more genetic characterization using whole‐genome sequencing (Burger et al, 2014; Low et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

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Infestation rates, seasonal distribution, and genetic diversity of ixodid ticks from livestock of various origins in two markets of Yaoundé, Cameroon

Yousseu

Tchetgna

Kamgang

et al. 2022

Medical Vet Entomology

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Little is known about the impact of ticks on livestock and humans in Cameroon. This study aimed to determine the prevalence, seasonal variation, and genetic diversity of hard ticks in the country. Ticks were collected during a cross-sectional survey on domestic livestock in two markets of Yaoundé in 2019 and 2020 and identified using morphological keys, 16S ribosomal DNA, (16S rDNA), and the cytochrome c oxidase subunit 1 (Cox1) genes.The infestation rates were 39.18%, 11.53%, and 2.74% in cattle, sheep, and goats respectively. Three genera of ticks were identified, Rhipicephalus, Amblyomma, and Hyalomma comprising eleven tick species. The main species were Rhipicephalus decoloratus (30.25%), R. microplus (24.43%), and Amblyomma variegatum (12.96%). Rhipicephalus spp. (81.31%) and Amblyomma variegatum (51.54%) were abundant during the rainy season, while Hyalomma spp. (83.86%) during the dry season (p-value <0.00001). Cox1 and 16S rDNA analysis showed a high level of genetic diversity among tick species with sequences close to those observed across Africa. Phylogenetic analysis revealed that our R. microplus belong to clade A and we identified R. sanguineus s.l. as R. linnea. This study shows a high tick infestation rate in cattle, while low in small ruminants with an extensive diversity of tick species, including several known vectors of important tick-borne diseases.

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“…En el COI se encuentran heredadas las características genéticas ascendentes, es por ello que el gen COI resulta ser el mejor objeto de estudio, ya que permite rastrear los ancestros de cualquier individuo (Paternina et al, 2016). De León et al (2011) reporta que existe un alto flujo genético entre las poblaciones del PAC al comparar registros de poblaciones de diferentes países como Sudáfrica, Estados Unidos de América, México, Argentina y Brasil.…”

Section: Introductionunclassified

Nuevos haplotipos de Diaphorina citri, vector de Candidatus Liberibacter en zonas citrícolas de México

Ceballos

Chávez

Fuentes

et al. 2022

Remexca

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Se identificaron nuevos haplotipos de Diaphorina citri también conocido como el psílido asiático de los cítricos, denominados DcitACC-1, DcitACC-2 y DcitACC-3. Los estudios se basaron en la amplificación de ADN del gen COI mitocondrial y se utilizaron individuos de diferentes zonas citrícolas del país, en algunas zonas productoras del país no se han realizado muestreos con anterioridad, específicamente en los municipios de Acatlán de Pérez, Oaxaca, Misantla y Tantoyuca, Veracruz y Huejutla, Hidalgo, por lo que se procedió a colectar insectos adultos sin distinción de género. El número de individuos de cada sitio colectado dependió de la disponibilidad de insectos en el lugar. Se obtuvieron en total 60 individuos colectados. La amplificación del ADN se realizó con los iniciadores específicos DCITRI COI-L y DCITRI COI-R, el producto de la reacción PCR se secuenció en el Instituto Potosino de Investigación Científica y Tecnológica, AC (IPICYT). Las secuencias obtenidas se compararon con las reportadas en el Genebank y se determinó que existe una línea matriz que corresponde a los haplotipos Dcit-01 y Dcit-04 con número de identificación FJ190300 y FJ190306 (Boykin, 2007). Se obtuvieron 22 secuencias que se analizaron con los programas Oligo analizer y Clustal Omega y se identificaron 11 secuencias iguales a los haplotipos Dcit-01 y Dcit-04. Los resultados mostraron 13 secuencias con diferencias en tres nucleótidos específicos: 61, 253 y 636, mismos que son reportados en este trabajo como nuevos haplotipos.

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Comparación y utilidad de las regiones mitocondriales de los genes 16S y COX1 para los análisis genéticos en garrapatas (Acari: Ixodidae)

Cited by 9 publications

References 66 publications

Trichuris trichiura (Linnaeus, 1771) From Human and Non-human Primates: Morphology, Biometry, Host Specificity, Molecular Characterization, and Phylogeny

Trichuris trichiura (Linnaeus, 1771) From Human and Non-human Primates: Morphology, Biometry, Host Specificity, Molecular Characterization, and Phylogeny

Infestation rates, seasonal distribution, and genetic diversity of ixodid ticks from livestock of various origins in two markets of Yaoundé, Cameroon

Nuevos haplotipos de Diaphorina citri, vector de Candidatus Liberibacter en zonas citrícolas de México

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