Compact coherent anti-Stokes Raman scattering microscope based on a picosecond two-color Er:fiber laser system

Krauss, Giinther; Hanke, Tobias; Sell, Alexander; Träutlein, Daniel; Leitenstorfer, Alfred; Selm, Romedi; Winterhalder, Martin; Zumbusch, Andreas

doi:10.1364/ol.34.002847

Cited by 101 publications

(71 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the LO power below 1 mW, the TAMP improved the SNR by up to 10 times compared to LIA based detection. Such improvement opens the opportunity for high-speed, high-quality SRS imaging with low power sources such as Er: fiber lasers [20], photonic crystal fiber [21,22] or fiber optical parametric oscillator [23] recently developed as compact and cost-effective alternatives to solid state lasers.…”

Section: Resultsmentioning

confidence: 99%

Heterodyne detected nonlinear optical imaging in a lock‐in free manner

Slipchenko

Oglesbee

Zhang

et al. 2012

Journal of Biophotonics

View full text Add to dashboard Cite

We report a compact, cost‐effective tuned amplifier for frequency‐selective amplification of the modulated signal in heterodyne detected nonlinear optical microscopy. Our method improved the signal to noise ratio by an order of magnitude compared to conventional lock‐in detection, as demonstrated through stimulated Raman scattering imaging of live cells and tissues at the speed of 2 μsec/pixel. Application of the tuned amplifier to transient absorption microscopy is also demonstrated. The increased signal to noise ratio allowed epi‐detected in vivo imaging of myelin and blood in rat spinal cord with high spatial resolution. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

Section: Resultsmentioning

confidence: 99%

Heterodyne detected nonlinear optical imaging in a lock‐in free manner

Slipchenko

Oglesbee

Zhang

et al. 2012

Journal of Biophotonics

View full text Add to dashboard Cite

show abstract

“…That's why PSF computes the characteristics of the image of point object (source) including the effects of diffraction. Since CARS intensity signals are treated at FWHM (full width at half maximum) which corresponds to the diameter of circle at 50 % encircled energy [10][11][12][13][14][15]21], we can say that FWHM is to be 0.72 µm for on-axis and 0.73 µm for off-axis end. However, CARS signal is actually proportional to the third power of laser beam intensity, namely I p …”

Section: Optical Performances and Discussionmentioning

confidence: 99%

“…NLO microscopy utilizes the signal from two-photon excitation fluorescence (TPEF) [1][2][3][4][5], second harmonic generation (SHG) [6][7], sum frequency generation (SFG) [8][9], or coherent anti-Stokes Raman scattering (CARS) [10][11][12][13][14][15], which can be combined with other techniques such as CARS microscopy with TPEF and SFG microscopy [11,13]. That's why the combined (or multimodal) microscopy can visualize the sub-cellular complex structures which cannot be resolved well by single NLO microscopy.…”

Section: Introductionmentioning

confidence: 99%

“…If the frequency difference, ω p -ω s , matches molecular-specific frequency resonantly, a blue-shifted and intense CARS signal is emitted at ω AS = 2ω p -ω s . From this CARS signal, three dimensional label-free images are obtained with sub-micrometer resolution [10][11][12][13][14][15]. In CARS microscopy reported until now, conventional microscope objectives are used, so that they are limited for introduction into a living body.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Optical Design of Miniaturized Microscope Objective for CARS Imaging Catheter with Fiber Bundle

Rim¹

2010

Journal of the Optical Society of Korea

View full text Add to dashboard Cite

In coherent anti-Stokes Raman scattering (CARS) microscopy reported until now, conventional microscope objectives are used, so that they are limited for introduction into a living body. Gradient-index (GRIN) rod lenses might be a solution for miniaturized microscope objectives for in-vivo CARS microscopy. However, due to the inherent large amount of chromatic aberration, GRIN rod lenses cannot be utilized for this purpose. CARS imaging catheter, composed of miniaturized microscope objective and fiber bundle, can be introduced into a living body for minimally invasive diagnosis. In order to design the catheter, we have to first investigate design requirements. And then, the optical design is processed with design strategies and intensive computing power to achieve the design requirements. We report the miniaturized objective lens system with diffraction-limited performance and completely corrected chromatic aberrations for an in-vivo CARS imaging catheter.

show abstract

“…Furthermore, the integration of spontaneous Raman spectroscopy onto the CARS microscope platform provides complete high-resolution Raman spectral information of the samples ( 47 ). Compact laser sources ( 38,117,118 ) are being developed to make CARS microscopy more accessible to biomedical researchers by reducing its cost, footprint, and complexity. Increasing the penetration depth with CARS endoscopy ( 84,85 ) or miniature microscope objective ( 119 ) could allow CARS imaging from the lumen of the intestine, esophagus, and artery, or CARS imaging of lipid-rich tissues with minimally invasive surgery.…”

Section: Study Of Myelin Diseasesmentioning

confidence: 99%

Shedding new light on lipid biology with coherent anti-Stokes Raman scattering microscopy

Yue

Cheng

2010

Journal of Lipid Research

147

130

View full text Add to dashboard Cite

Despite the ubiquitous roles of lipids in biology, the detection of lipids has relied on invasive techniques and population measurements. The molecular profi les of the lipid-rich structures are evaluated by measurements of total lipid extracts with liquid or gas chromatography coupled to mass spectrometry ( 8 ). This approach is inexact because it measures lipid molecules from areas other than the structures of interest. Furthermore, spatial information, which is critical to understanding the function of lipids ( 9 ), is inaccessible with the bulk measurement techniques. To visualize the lipid-rich structures, fl uorescently tagged lipid molecules, which intercalate with the lipidrich structures, are used ( 10, 11 ). Nevertheless, the use of the fl uorescent lipid molecules to label lipid-rich structures is problematic for several reasons. First, to facilitate the transport of the fl uorescent lipid molecules into cells, cell fi xation procedures are often performed ( 12 ). This procedure prevents monitoring the dynamic responses of lipid-rich structures to stimuli or the disease processes. Second, labeling effi ciency is highly dependent on the type of fl uorescent lipid molecules ( 12 ), thus posing a signifi cant problem to the quantitation of lipid-rich structures. Third, the intercalation of fl uorescent lipid molecules can lead to changes in the properties of lipidrich structures. For instance, incorporation of fl uorescent lipid molecules into the cell membrane can induce membrane phase separation and membrane microdomain formation, which can perturb critical cellular processes including signal transduction and endocytosis ( 13 ).As an alternative to fl uorescence, signals from molecular vibration provide an attractive means for label-free chemical imaging. In particular, Raman scattering has been widely used for spectroscopic study of biomolecules ( 14 ). The Raman-scattered photons exhibit frequency Abstract Despite the ubiquitous roles of lipids in biology, the detection of lipids has relied on invasive techniques, population measurements, or nonspecifi c labeling. Such diffi culties can be circumvented by a label-free imaging technique known as coherent anti-Stokes Raman (CARS) microscopy, which is capable of chemically selective, highly sensitive, and high-speed imaging of lipid-rich structures with submicron three-dimensional spatial resolution. We review the broad applications of CARS microscopy to studies of lipid biology in cell cultures, tissue biopsies, and model organisms. Recent technical advances, limitations of the technique, and perspectives are discussed. Lipids play a critical role in human health and diseases. Phospholipids, glycolipids, and sterol lipids are the major components of the cell membrane ( 1 ). Sphingolipids constitute up to 80% of the myelin sheaths, which are the membranous structures that wrap around the axons for insulation and for proper propagation of electrical impulses ( 2 ). Glycerolipids or triglycerides serve as the cytoplasmic energy depots ( 3 ). Bioactive lip...

show abstract

Compact coherent anti-Stokes Raman scattering microscope based on a picosecond two-color Er:fiber laser system

Cited by 101 publications

References 21 publications

Heterodyne detected nonlinear optical imaging in a lock‐in free manner

Heterodyne detected nonlinear optical imaging in a lock‐in free manner

The Optical Design of Miniaturized Microscope Objective for CARS Imaging Catheter with Fiber Bundle

Shedding new light on lipid biology with coherent anti-Stokes Raman scattering microscopy

Contact Info

Product

Resources

About