Heterodyne detected nonlinear optical imaging in a lock‐in free manner

Slipchenko, Mikhail N.; Oglesbee, Robert A.; Zhang, De‐Long; Wu, Wei; Cheng, Ji‐Xin

doi:10.1002/jbio.201200005

Cited by 65 publications

(63 citation statements)

References 24 publications

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“…Another advance in instrumentation was an alternative to the lock-in detection of the modulated light, instead using a tunable amplifier (TAMP). This reduced the Johnson-Nyquist noise, which dominates over shot noise at low laser power, compared with lock-in techniques, showing an order of magnitude improvement in signal-to-noise [247]. This was also extended to a TAMP array to allow multiplexed SRS measurements without using multiple lock-in amplifiers [248].…”

Section: Typical Systemmentioning

confidence: 98%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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Section: Typical Systemmentioning

confidence: 98%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

View full text Add to dashboard Cite

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“…For SRS imaging, we detected the stimulated Raman loss (SRL) signal, where the Stokes beam was blocked by a bandpass filter (825∕150 nm, Chroma Technologies, Bellows Falls, Vermont). The signal in the pump beam was then amplified with a resonant amplifier 10,31 and extracted by a lock-in amplifier (HF2LI, Zurich Instrument, Switzerland). The wavelengths of the pump and Stokes beams were selected to 830 and 1087 nm, respectively, and the frequency difference of 2854 cm −1 resonated with the symmetric stretch of CH 2 .…”

Section: Srs Microscopementioning

confidence: 99%

Label-free real-time imaging of myelination in theXenopus laevistadpole byin vivostimulated Raman scattering microscopy

Zhang

Slipchenko

et al. 2014

J. Biomed. Opt

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Abstract. The myelin sheath plays an important role as the axon in the functioning of the neural system, and myelin degradation is a hallmark pathology of multiple sclerosis and spinal cord injury. Electron microscopy, fluorescent microscopy, and magnetic resonance imaging are three major techniques used for myelin visualization. However, microscopic observation of myelin in living organisms remains a challenge. Using a newly developed stimulated Raman scattering microscopy approach, we report noninvasive, label-free, real-time in vivo imaging of myelination by a single-Schwann cell, maturation of a single node of Ranvier, and myelin degradation in the transparent body of the Xenopus laevis tadpole.

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“…A tuned amplifier is a compact alternative for frequency selective signal amplification as in SRS allowing pixel dwell times of 2 ms [101].…”

Section: Speedmentioning

confidence: 99%

Accumulating advantages, reducing limitations: Multimodal nonlinear imaging in biomedical sciences – The synergy of multiple contrast mechanisms

Meyer

Schmitt

Dietzek

et al. 2013

Journal of Biophotonics

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Journal of BIOPHOTONICSMultimodal nonlinear microscopy has matured during the past decades to one of the key imaging modalities in life science and biomedicine due to its unique capabilities of label-free visualization of tissue structure and chemical composition, high depth penetration, intrinsic 3D sectioning, diffraction limited resolution and low phototoxicity. This review briefly summarizes first recent advances in the field regarding the methodology, e.g., contrast mechanisms and signal characteristics used for contrast generation as well as novel image processing approaches. The second part deals with technologic developments emphasizing improvements in penetration depth, imaging speed, spatial resolution and nonlinear labeling strategies. The third part focuses on recent applications in life science fundamental research and biomedical diagnostics as well as future clinical applications.Multimodal nonlinear microscopy of tissue.

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Heterodyne detected nonlinear optical imaging in a lock‐in free manner

Cited by 65 publications

References 24 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

Label-free real-time imaging of myelination in theXenopus laevistadpole byin vivostimulated Raman scattering microscopy

Accumulating advantages, reducing limitations: Multimodal nonlinear imaging in biomedical sciences – The synergy of multiple contrast mechanisms

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