Accumulating advantages, reducing limitations: Multimodal nonlinear imaging in biomedical sciences – The synergy of multiple contrast mechanisms

Meyer, Tobias; Schmitt, Michael; Dietzek, Benjamin; Popp, Jürgen

doi:10.1002/jbio.201300176

Cited by 32 publications

(29 citation statements)

References 162 publications

(234 reference statements)

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“…The SH output showed no distortion and was diffraction limited, despite the non-Gaussian input fundamental light. This configuration would indeed be applicable for power scaling purposes for practical use, e.g., in multimodal bioimaging applications [19,20].…”

Section: Discussionmentioning

confidence: 99%

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Concept for power scaling second harmonic generation using a cascade of nonlinear crystals

et al. 2015

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light from a tapered laser diode, while avoiding significant thermal effects. Besides constituting the highest SH power yet achieved using a laser diode, this demonstrates that the concept successfully combines the high efficiency of the first stage with the good power handling properties of the subsequent stages. The concept is generally applicable and can be expanded with more stages to obtain even higher efficiency, and extends also to other combinations of nonlinear media suitable for other wavelengths.

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Section: Discussionmentioning

confidence: 99%

“…Uses of titanium sapphire lasers such as the emerging field of single-source multimodal bioimaging [19,20] place high demands on the power of the titanium sapphire laser and, in turn, the power of its pump source.…”

Section: Introductionmentioning

confidence: 99%

Concept for power scaling second harmonic generation using a cascade of nonlinear crystals

et al. 2015

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“…The nonlinear optical microscopy techniques have been recognized as fast imaging tools without the need for external markers and with the possibility of optical sectioning (125). However, the amount of techniques successfully applied to tissue diagnostics is rapidly increasing such that a thorough description would exceed the scope of this review.…”

Section: Multiphoton/multimodal Nonlinear Optical Microscopymentioning

confidence: 99%

Multimodal Imaging Spectroscopy of Tissue

Vogler

Heuke

Bocklitz

et al. 2015

Annual Rev. Anal. Chem.

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Advanced optical imaging technologies have experienced increased visibility in medical research, as they allow for a label-free and nondestructive investigation of tissue in either an excised state or living organisms. In addition to a multitude of ex vivo studies proving the applicability of these optical imaging approaches, a transfer of various modalities toward in vivo diagnosis is currently in progress as well. Furthermore, combining optical imaging techniques, referred to as multimodal imaging, allows for an improved diagnostic reliability due to the complementary nature of retrieved information. In this review, we provide a summary of ongoing multifold efforts in multimodal tissue imaging and focus in particular on in vivo applications for medical diagnosis. We also discuss the advantages and potential limitations of the imaging methods and outline opportunities for future developments.

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“…Among others, they include customized organic dyes and quantum dots for one‐ and two‐photon‐excited fluorescence, perovskite‐type nanocrystals as harmonic probes for SHG, and gold and silver nanoparticles that can be used as plasmonic probes for SEHRS imaging of cells and tissues . The combination of linear and nonlinear microspectroscopy, such as of SHG with two‐photon fluorescence and conventional RS,4c,14 or of SERS with SEHRS,7c,13a creates a strong demand for nanoprobes with multiple optical modalities, in order to provide complementary and more reliable information by joining the strengths of different spectroscopic methods . Extending the capabilities of nanoprobes that deliver chemical information through a vibrational signature with the requirements of fast nonlinear imaging would allow truly multimodal optical imaging based on both chemical structural information with morphology at the microscopic level, and thus could be very beneficial for microspectroscopic studies of biological objects.…”

Section: Introductionmentioning

confidence: 99%

Gold‐ and Silver‐Coated Barium Titanate Nanocomposites as Probes for Two‐Photon Multimodal Microspectroscopy

Madzharova

Nodar

Živanović

et al. 2019

Adv Funct Materials

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Improved multiphoton-excited imaging and microspectroscopy require nanoprobes that can give different nonlinear optical signals. Here, composite nanostructures with a barium titanate core and a plasmonic moiety at their surface are synthesized and characterized. It is found that the core provides a high second-order nonlinear susceptibility for sensitive second harmonic generation (SHG) imaging in living cells. As a second function in the twophoton regime, the plasmonic part yields high local fields for resonant and nonresonant surface enhanced hyper Raman scattering (SEHRS). SEHRS complements the one-photon surface enhanced Raman scattering (SERS) spectra that are also enhanced by the plasmonic shells. Barium titanate silver core-shell (Ag@BaTiO 3 ) composites are specifically suited for SEHRS and SHG excited at 1064 nm, while gold at barium titanate (Au@BaTiO 3 ) nanoparticles can be useful in a combination of SHG and SERS at lower wavelengths, here at 785 nm and 850 nm. The theoretical models show that the optical properties of the BaTiO 3 dielectric core depend on probing frequency, shape, size, and plasmonic properties of the surrounding gold nanoparticles so that they can be optimized for a particular type of experiment. These versatile, tunable probes give new opportunities for combined multiphoton probing of morphological structure and chemical properties of biosystems.very attractive in laser microspectroscopy and imaging. They include two-photonexcited fluorescence, [3] second harmonic generation (SHG), [4] stimulated Raman scattering [5] and coherent anti-Stokes Raman scattering [6] microscopies, and imaging based on spontaneous hyper Raman scattering (HRS). [7] Nonlinear processes are attractive in microscopy and spectroscopy since they can be excited with light in the near-infrared, which offers several advantages. The latter include deep tissue penetration capability, reduced photo damage due to the lower photon energy, and spatially more confined probed volume, which can result in an improved lateral resolution. [8] Nevertheless, when multiphoton-excited processes are complemented with their one-photon-excited counterparts, this can yield unprecedented structural information from a sample. As an example, the spontaneous twophoton-excited Raman process, termed HRS, is governed by different selection rules than the (conventional) spontaneous one-photon-excited RS. Both HRS and RS can be enhanced in the local fields of plasmonic nanostructures, resulting in surface enhanced hyper Raman scattering (SEHRS) [9] and surface enhanced Raman scattering (SERS) spectra, respectively. While imaging based on vibrational spectra gives detailed molecular structure, other nonlinear optical signals, such as SHG, are less specific but lead to information about the microscopic morphology of a sample. SHG is a two-photon process, where a

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Accumulating advantages, reducing limitations: Multimodal nonlinear imaging in biomedical sciences – The synergy of multiple contrast mechanisms

Cited by 32 publications

References 162 publications

Concept for power scaling second harmonic generation using a cascade of nonlinear crystals

Concept for power scaling second harmonic generation using a cascade of nonlinear crystals

Multimodal Imaging Spectroscopy of Tissue

Gold‐ and Silver‐Coated Barium Titanate Nanocomposites as Probes for Two‐Photon Multimodal Microspectroscopy

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