Abstract:Background
The measurement of hemoglobin A1c (HbA1c) is important for diagnosing diabetes mellitus as well as assessing glycemic control in diabetic patients. Commutable whole blood certified reference materials (CRMs) are needed in the measurement of HbA1c for method validation and/or as quality controls.
Methods
We developed three levels of hemolyzed whole blood CRMs for HbA1c. The certified values were determined using liquid chromatography-isotope dilution tandem mass spectrometry method (LC-IDMS/MS) wher… Show more
“…Isotope Dilution Mass spectrometry (IDMS) is widely applied for protein absolute quantification [66,[71][72][73][74][75][76][77][78][79]. Thanks to its high selectivity and the use of stable-isotopelabelled (SIL) compounds as internal standards, IDMS allows accurate quantification of the measurand with low measurement uncertainties and is recognized as a primary reference method by the Consultative Committee for Amount of Substance of BIPM (Bureau International des Poids et Mesures).…”
Section: Selection and Development Of Candidate Reference Measurement Systemmentioning
“…Isotope Dilution Mass spectrometry (IDMS) is widely applied for protein absolute quantification [66,[71][72][73][74][75][76][77][78][79]. Thanks to its high selectivity and the use of stable-isotopelabelled (SIL) compounds as internal standards, IDMS allows accurate quantification of the measurand with low measurement uncertainties and is recognized as a primary reference method by the Consultative Committee for Amount of Substance of BIPM (Bureau International des Poids et Mesures).…”
Section: Selection and Development Of Candidate Reference Measurement Systemmentioning
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