Comparative study on quantitation of human myoglobin by both isotope dilution mass spectrometry and surface plasmon resonance based on calibration-free analysis

Hu, Tingting; Wu, Liqing; Sun, Xiaonan; Su, Ping; Yang, Yi

doi:10.1007/s00216-020-02504-z

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…CFCA was also used to evaluate the potency of TNF-α before and after temperature stress, proving an alternative to a traditional EC 50 analysis [37]. Absolute CFCA was recently used to quantify human myoglobin with good agreement with isotope dilution mass spectrometry measurements [99], and a mathematical framework was developed to quantify analytes prone to self-association [100]. CFCA, especially in a relative framework, has already been proven to be efficient and accurate, and we predict its use will increase in the near future.…”

Section: Spr To Measure Concentrationsmentioning

confidence: 99%

On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring

et al. 2021

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Biomanufacturers are being incited by regulatory agencies to transition from a quality by testing framework, where they extensively test their product after their production, to more of a quality by design or even quality by control framework. This requires powerful analytical tools and sensors enabling measurements of key process variables and/or product quality attributes during production, preferably in an online manner. As such, the demand for monitoring technologies is rapidly growing. In this context, we believe surface plasmon resonance (SPR)-based biosensors can play a role in enabling the development of improved bioprocess monitoring and control strategies. The SPR technique has been profusely used to probe the binding behavior of a solution species with a sensor surface-immobilized partner in an investigative context, but its ability to detect binding in real-time and without a label has been exploited for monitoring purposes and is promising for the near future. In this review, we examine applications of SPR that are or could be related to bioprocess monitoring in three spheres: biotherapeutics production monitoring, vaccine monitoring, and bacteria and contaminant detection. These applications mainly exploit SPR’s ability to measure solution species concentrations, but performing kinetic analyses is also possible and could prove useful for product quality assessments. We follow with a discussion on the limitations of SPR in a monitoring role and how recent advances in hardware and SPR response modeling could counter them. Mainly, throughput limitations can be addressed by multi-detection spot instruments, and nonspecific binding effects can be alleviated by new antifouling materials. A plethora of methods are available for cell growth and metabolism monitoring, but product monitoring is performed mainly a posteriori. SPR-based biosensors exhibit potential as product monitoring tools from early production to the end of downstream processing, paving the way for more efficient production control. However, more work needs to be done to facilitate or eliminate the need for sample preprocessing and to optimize the experimental protocols.

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Section: Spr To Measure Concentrationsmentioning

confidence: 99%

On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring

et al. 2021

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“…However, the SA method is time-consuming, complex, and costly due to the need for additional reagents and steps, often involving a full calibration curve with several successive standard additions for each sample to be analyzed . In practice, when a SA-based analytical method is used routinely and high efficiency is required in terms of time, workload, and resources, this approach is reduced to one- or two-point calibrations, along with a reduced number of replicas at each level, as long as the calibration curve has been previously well-characterized by proper statistical treatment and method validation to ensure the reliability and quality of the analytical results. − Recent efforts have pursued reducing the extent of the calibration step , or even its elimination through implementation of calibration-free sensors. ,− Calibration-free sensors have been demonstrated to be feasible but not without requiring a highly robust development process involving the optimization of sensing materials, fabrication and assay protocols, and the use of the most adequate readout methodologies . The use of SA is particularly time-consuming in the case of immunoassays because each measurement typically requires 20–30 min (primarily as incubation time for capturing the target antigen), leading to complete assay times of over 60 or 90 min (along with the corresponding related labor and reagent costs).…”

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confidence: 99%

Concept of the “Universal Slope”: Toward Substantially Shorter Decentralized Insulin Immunoassays

et al. 2022

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Decentralized sensing of analytes in remote locations is today a reality. However, the number of measurable analytes remains limited, mainly due to the requirement for time-consuming successive standard additions calibration used to address matrix effects and resulting in greatly delayed results, along with more complex and costly operation. This is particularly challenging in commonly used immunoassays of key biomarkers that typically require from 60 to 90 min for quantitation based on two standard additions, hence hindering their implementation for rapid and routine diagnostic applications, such as decentralized point-of-care (POC) insulin testing. In this work we have developed and demonstrated the theoretical framework for establishing a universal slope for direct calibration-free POC insulin immunoassays in serum samples using an electrochemical biosensor (developed originally for extended calibration by standard additions). The universal slope is presented as an averaged slope constant, relying on 68 standard additions-based insulin determinations in human sera. This new quantitative analysis approach offers reliable sample measurement without successive standard additions, leading to a dramatically simplified and faster assay (30 min vs 90 min when using 2 standard additions) and greatly reduced costs, without compromising the analytical performance while significantly reducing the analyses costs. The substantial improvements associated with the new universal slope concept have been demonstrated successfully for calibration-free measurements of serum insulin in 30 samples from individuals with type 1 diabetes using meticulous statistical analysis, supporting the prospects of applying this immunoassay protocol to routine decentralized POC insulin testing.

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Development of cancer biomarker heat shock protein 90α certified reference material using two different isotope dilution mass spectrometry techniques

Zhu,

Li,

Chu

et al. 2023

Anal Bioanal Chem

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Comparative study on quantitation of human myoglobin by both isotope dilution mass spectrometry and surface plasmon resonance based on calibration-free analysis

Cited by 4 publications

References 25 publications

On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring

On the Use of Surface Plasmon Resonance-Based Biosensors for Advanced Bioprocess Monitoring

Concept of the “Universal Slope”: Toward Substantially Shorter Decentralized Insulin Immunoassays

Development of cancer biomarker heat shock protein 90α certified reference material using two different isotope dilution mass spectrometry techniques

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