Communication via gap junctions underlies early functional interactions between grafted neural stem cells and the host

Jäderstad, Johan; Danielsson, Louise; Ourednik, Václav; Snyder, Evan Y.; Herlenius, Eric

doi:10.1016/j.expneurol.2006.02.050

Cited by 3 publications

(2 citation statements)

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“…2D.13.6), FRAP analysis (fluorescence recovery after photobleaching; Wade et al, 1986), electrocoupling (Loewenstein, 1979), and transfer of radioactive metabolites (Hooper, 1982). We have successfully employed the first four of these methods in the currently described systems (Jäderstad et al, 2006(Jäderstad et al, , 2010a(Jäderstad et al, ,b,c, 2011.…”

Section: Dye Spread To Investigate Direct Intercellular Connectionsmentioning

confidence: 99%

“…Due to the preserved tissue cytoarchitecture, an organotypic brain slice culture is suitable to study cell‐to‐cell interactions including incorporation and differentiation of NSC in host tissues (Scheffler et al, ). We have established conditions for propagation of rat auditory brainstem slice cultures containing the cochlear nucleus (CN) and used striatal roller drum cultures to determine how stem cells interact with host tissue (Jäderstad et al, , , ,b,c, ; Thonabulsombat et al, ; Glavaski‐Joksimovic et al, ). Migration of NSC has been shown in cultures with brainstem as well as hippocampal slices using the interface method (Benninger et al, ; Scheffler et al, ; Glavaski‐Joksimovic et al, ).…”

Section: Commentarymentioning

confidence: 99%

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Functional Stem Cell Integration Assessed by Organotypic Slice Cultures

Herlenius

Thonabulsombat

Forsberg

et al. 2012

CP Stem Cell Biology

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Re‐formation or preservation of functional, electrically active neural networks has been proffered as one of the goals of stem cell‐mediated neural therapeutics. A primary issue for a cell therapy approach is the formation of functional contacts between the implanted cells and the host tissue. Therefore, it is of fundamental interest to establish protocols that allow us to delineate a detailed time course of grafted stem cell survival, migration, differentiation, integration, and functional interaction with the host. One option for in vitro studies is to examine the integration of exogenous stem cells into an existing active neuronal network in ex vivo organotypic cultures. Organotypic cultures leave the structural integrity essentially intact while still allowing the microenvironment to be carefully controlled. This allows detailed studies over time of cellular responses and cell‐cell interactions, which are not readily performed in vivo. This unit describes procedures for using organotypic slice cultures as ex vivo model systems for studying neural stem cell and embryonic stem cell engraftment and communication with CNS host tissue. Curr. Protoc. Stem Cell Biol. 23:2D.13.1‐2D.13.26. © 2012 by John Wiley & Sons, Inc.

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Section: Dye Spread To Investigate Direct Intercellular Connectionsmentioning

confidence: 99%

Section: Commentarymentioning

confidence: 99%

Functional Stem Cell Integration Assessed by Organotypic Slice Cultures

Herlenius

Thonabulsombat

Forsberg

et al. 2012

CP Stem Cell Biology

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Dynamic changes in connexin expression following engraftment of neural stem cells to striatal tissue

Jäderstad

Herlenius

2011

Experimental Cell Research

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Graft and Host Interactions Following Transplantation of Neural Stem Cells to Organotypic Striatal Cultures

Jäderstad

Herlenius³

2010

Regen. Med.

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Aims: To investigate neural stem cell (NSC) interactions with striatal tissue following engraftment and the effects of growth factors. Materials & methods: Organotypic striatal slice cultures established from neonatal rats were used as an ex vivo model system. Survival, integration and differentiation of grafted NSCs from the previously generated C17.2 clone and host tissue response were investigated weekly for 28 days in vitro. To direct grafted cells towards a neuronal lineage, the role of growth factor supplementation and serum-free culturing conditions was studied using neural stem cells overexpressing neurotrophin-3 and Neurobasal™/B27 culture medium. Results: Following engraftment, NSCs gradually integrated morphologically and formed a part of the host 3D cytoarchitecture. Compared with nongrafted cultures, NSC engraftment increased the overall survival of the organotypic cultures by 39%, and reduced the host cell necrosis by more than 80% (from 2.1 ± 0.5% to 0.3 ± 0.1%), the host cell apoptosis by more than 60% (from 1.4 ± 0.4% to 0.5 ± 0.1%) and the reactions to mechanical trauma by 30% (estimated by nestin and glial fibrillary acidic protein immunohistochemistry) 7 days after engraftment. Elevated neurotrophin-3 production in NSCs and serum-free culturing conditions directed grafted NSCs towards a neuronal lineage as indicated by increased Tuj1 and Map2ab expression. However, this did not alter the survival of organotypic cultures. Conclusions: NSC engraftment was associated with rescue of imperiled host cells and reduction of host cell gliosis. These NSC effects were not related to the addition of growth factors, suggesting that other factors are involved in the supportive effects of the host following NSC engraftment.

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Communication via gap junctions underlies early functional interactions between grafted neural stem cells and the host

Cited by 3 publications

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Functional Stem Cell Integration Assessed by Organotypic Slice Cultures

Functional Stem Cell Integration Assessed by Organotypic Slice Cultures

Dynamic changes in connexin expression following engraftment of neural stem cells to striatal tissue

Graft and Host Interactions Following Transplantation of Neural Stem Cells to Organotypic Striatal Cultures

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