Graft and Host Interactions Following Transplantation of Neural Stem Cells to Organotypic Striatal Cultures

Jäderstad, Linda Maria; Jäderstad, Johan; Herlenius, Eric

doi:10.2217/rme.10.80

Cited by 10 publications

(35 citation statements)

References 64 publications

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“…Gap-junctional intercellular networks are an early form of communication that precedes and sets the stage for later electrochemical synapses and “traditional” electrophysiological communication between grafted and host cells. It is the earlier connexin, Cx43, that seems most pivotal in the NSCs-mediated rescue actions [7, 23]. …”

Section: Discussionmentioning

confidence: 99%

Experimental research Enhanced connexin 43 expression following neural stem cell transplantation in a rat model of traumatic brain injury

Ma²,

Kong³

et al. 2013

aoms

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IntroductionReestablishment of functional networks after traumatic brain injury (TBI) has been proffered as one of the goals of neural stem cell (NSC) transplantation therapeutics. Gap junctions provide essential means for direct cellular communication by transferring small molecules and ions, which may provide insights into the interplay between grafted NSCs and host cells.Material and methodsThirty-six adult male Wister rats were used in this study. The controlled cortical impact (CCI) model of brain injury has been performed. Seventy-two hours after CCI injury, animals were randomly assigned to two groups: PBS- and NSC- transplanted group. NSCs-transplanted group received delivery of the NSCs suspension to the cortex below the injury cavity in the ipsilateral hemisphere. At 1, 2, and 4 weeks post-transplantation, we investigated the expression patterns of gap junction-associated connexin 43 (Cx43) in the transplant site and the border of CCI by immunohistochemistry, Western blot and RT-PCR.ResultsOur findings showed that Cx43 staining was significantly greater in the transplant site and the border of CCI in the NSCs-transplanted rats compared to the control rats at different time points (p < 0.01 at 1 week, p < 0.05 at 2 and 4 weeks). Significantly higher gene and protein expression of Cx43 was found in NSCs-transplanted rats compared to the control rats in the period of 4 weeks post-transplantation (p < 0.01), and remained at a higher level until 2 weeks with or without NSC transplantation.ConclusionsIt is proposed that gap junction-associated Cx43 might participate in NSCs’ beneficial effects via gap-junctional coupling by which grafted NSCs integrate into host neural tissue following transplantation after TBI.

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Section: Discussionmentioning

confidence: 99%

Experimental research Enhanced connexin 43 expression following neural stem cell transplantation in a rat model of traumatic brain injury

Ma²,

Kong³

et al. 2013

aoms

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“…[125,126] Moreover, it is also feasible to investigate the electrophysiological properties as well as calcium and/or magnesium measurements using such brain organotypic slices models. [127,128] Despite all the advantages presented so far, organotypic brain slices show key disadvantages that halted people from using them widely as a standard model. These models can be maintained in cultures only for few weeks; they are very thin and fragile tissue (≈100–400 μm) and they can be distorted during the culture maintenance.…”

Section: Recapitulating the Human Brain Pathophysiologymentioning

confidence: 99%

Three‐Dimensional Models of the Human Brain Development and Diseases

Jorfi

D’Avanzo

Kim

et al. 2017

Adv Healthcare Materials

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Deciphering the human brain pathophysiology remains one of the greatest challenges of the 21st century. Neurological disorders represent a significant proportion of diseases burden; however, the complexity of the brain physiology makes it challenging to model its diseases. Simple in vitro models have been very useful for precise measurements in controled conditions. However, existing models are limited in their ability to replicate complex interactions between various cells in the brain. Studying human brain requires sophisticated models to reconstitute the tangled architecture and functions of brain cells. Recently, advances in the development of three-dimensional (3D) brain cell culture models have begun to recapitulate various aspects of the human brain physiology in vitro and replicate basic disease processes of Alzheimer’s disease, amyotrophic lateral sclerosis, and microcephaly. In this review, we discuss the progress, advantages, limitations, and future directions of 3D cell culture systems for modeling the human brain development and diseases.

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“…2D.13.6), FRAP analysis (fluorescence recovery after photobleaching; Wade, Trosko, & Schindler, 1986), electrocoupling (Loewenstein, 1979), and transfer of radioactive metabolites (Hooper, 1982). We have successfully employed the first four of these methods in the currently described systems (Jäderstad et al, 2010a;Jäderstad et al, 2011;Jäderstad et al, 2010b;Jäderstad et al, 2010c).…”

Section: Dye Spread To Investigate Direct Intercellular Connectionsmentioning

confidence: 99%

“…We also quantify the number of host cells receiving calcein manually, in a blinded fashion (see Fig. 2D.13.7 and Jäderstad et al, 2010b;Jäderstad et al, 2010c).…”

Section: Trypsinize Nscsmentioning

confidence: 99%

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Functional Stem Cell Integration into Neural Networks Assessed by Organotypic Slice Cultures

Forsberg

Thonabulsombat

Jäderstad

et al. 2017

CP Stem Cell Biology

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Re-formation or preservation of functional, electrically active neural networks has been proffered as one of the goals of stem cell-mediated neural therapeutics. A primary issue for a cell therapy approach is the formation of functional contacts between the implanted cells and the host tissue. Therefore, it is of fundamental interest to establish protocols that allow us to delineate a detailed time course of grafted stem cell survival, migration, differentiation, integration, and functional interaction with the host. One option for in vitro studies is to examine the integration of exogenous stem cells into an existing active neural network in ex vivo organotypic cultures. Organotypic cultures leave the structural integrity essentially intact while still allowing the microenvironment to be carefully controlled. This allows detailed studies over time of cellular responses and cell-cell interactions, which are not readily performed in vivo. This unit describes procedures for using organotypic slice cultures as ex vivo model systems for studying neural stem cell and embryonic stem cell engraftment and communication with CNS host tissue. © 2017 by John Wiley & Sons, Inc.

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Graft and Host Interactions Following Transplantation of Neural Stem Cells to Organotypic Striatal Cultures

Cited by 10 publications

References 64 publications

Experimental research Enhanced connexin 43 expression following neural stem cell transplantation in a rat model of traumatic brain injury

Experimental research Enhanced connexin 43 expression following neural stem cell transplantation in a rat model of traumatic brain injury

Three‐Dimensional Models of the Human Brain Development and Diseases

Functional Stem Cell Integration into Neural Networks Assessed by Organotypic Slice Cultures

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