Functional Stem Cell Integration into Neural Networks Assessed by Organotypic Slice Cultures

Forsberg, David; Thonabulsombat, Charoensri; Jäderstad, Johan; Jäderstad, Linda Maria; Olivius, Petri; Herlenius, Eric

doi:10.1002/cpsc.34

Cited by 7 publications

(4 citation statements)

References 111 publications

(185 reference statements)

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“…The main function of NSCs is to serve as a cellular reserve to participate in the repair of nervous system damage or to replace dead cells (Forsberg et al, 2017). The hippocampus is the part of the brain involved in bodily sensations, learning, memory, and homeostasis, and hippocampal NSCs have been used for decades as a typical model of the nervous system (Hattiangady and Shetty, 2012).…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation

Fang

Zhang

Chen

et al. 2020

Front. Bioeng. Biotechnol.

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Graphene consists of two-dimensional sp2-bonded carbon sheets, a single or a few layers thick, which has attracted considerable interest in recent years due to its good conductivity and biocompatibility. Three-dimensional graphene foam (3DG) has been demonstrated to be a robust scaffold for culturing neural stem cells (NSCs) in vitro that not only supports NSCs growth, but also maintains cells in a more active proliferative state than 2D graphene films and ordinary glass. In addition, 3DG can enhance NSCs differentiation into astrocytes and especially neurons. However, the underlying mechanisms behind 3DG's effects are still poorly understood. Metabolism is the fundamental characteristic of life and provides substances for building and powering the cell. Metabolic activity is tightly tied with the proliferation, differentiation, and self-renewal of stem cells. This study focused on the metabolic reconfiguration of stem cells induced by culturing on 3DG. This study established the correlation between metabolic reconfiguration metabolomics with NSCs cell proliferation rate on different scaffold. Several metabolic processes have been uncovered in association with the proliferation change of NSCs. Especially, culturing on 3DG triggered pathways that increased amino acid incorporation and enhanced glucose metabolism. These data suggested a potential association between graphene and pathways involved in Parkinson's disease. Our work provides a very useful starting point for further studies of NSC fate determination on 3DG.

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Section: Discussionmentioning

confidence: 99%

Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation

Fang

Zhang

Chen

et al. 2020

Front. Bioeng. Biotechnol.

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“…Mouse pups were used at postnatal day 3 for the establishment of brainstem organotypic slice cultures ( Forsberg et al, 2016 ; Forsberg et al, 2017 ). Transverse slices (300 µm thick) were maintained in culture for 7 to 14 days.…”

Section: Methodsmentioning

confidence: 99%

Astrocytes release prostaglandin E2 to modify respiratory network activity

Forsberg

Ringstedt

Herlenius

2017

eLife

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Previously (Forsberg et al., 2016), we revealed that prostaglandin E2 (PGE2), released during hypercapnic challenge, increases calcium oscillations in the chemosensitive parafacial respiratory group (pFRG/RTN). Here, we demonstrate that pFRG/RTN astrocytes are the PGE2 source. Two distinct astrocyte subtypes were found using transgenic mice expressing GFP and MrgA1 receptors in astrocytes. Although most astrocytes appeared dormant during time-lapse calcium imaging, a subgroup displayed persistent, rhythmic oscillating calcium activity. These active astrocytes formed a subnetwork within the respiratory network distinct from the neuronal network. Activation of exogenous MrgA1Rs expressed in astrocytes tripled astrocytic calcium oscillation frequency in both the preBötzinger complex and pFRG/RTN. However, neurons in the preBötC were unaffected, whereas neuronal calcium oscillatory frequency in pFRG/RTN doubled. Notably, astrocyte activation in pFRG/RTN triggered local PGE2 release and blunted the hypercapnic response. Thus, astrocytes play an active role in respiratory rhythm modulation, modifying respiratory-related behavior through PGE2 release in the pFRG/RTN.

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“…In order to investigate the neuronal differentiation of DPSC-derived neural precursor cells (DPSC-NPC) and SHED-derived neural precursor cells (SHED-NPC), a transplantation of the cells was conducted followed a currently published protocol (Forsberg et al, 2017). The spheres with 100-200 μm diameter size were transplanted adjacent to CN of ABS (coculture) after 5 ± 2 days ABS slice culturing.…”

Section: Neural Precursor Cell In Monoculture and Coculture With Absmentioning

confidence: 99%

Neuronal differentiation of dental pulp stem cells from human permanent and deciduous teeth following coculture with rat auditory brainstem slices

Gonmanee

Sritanaudomchai

Vongsavan

et al. 2020

The Anatomical Record

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Sensorineural hearing loss is a common disability found worldwide which is associated with a degeneration of spiral ganglion neurons (SGN). It is a challenge to restore SGN due to the permanent degeneration and viability of SGN is requisite for patients to receive an advantage from hearing aid devices. Human dental pulp stem cells (DPSC) and stem cells from human exfoliated deciduous teeth (SHED) are self-renewing stem cells that originate from the neural crest during development. These stem cells have a high potential for neuronal differentiation. This is primarily due to their multilineage differentiation potential and their relative ease of access. Previously, we have shown the ability of these stem cell types to differentiate into spiral ganglion neuron-like cells. In this study, we induced the cells into neural precursor cells (NPC) and cocultured with auditory brainstem slice (ABS) encompassing cochlear nucleus by the Stoppini method. We also investigated their ability to differentiate after 2 weeks and 4 weeks in coculture. Neuronal differentiation of DPSC-NPC and SHED-NPC was higher expression of specific markers to SGN, TrkB, and Gata3, compared to monoculture. The cells also highly expressed synaptic vesicle protein (SV2A) and exhibited intracellular calcium oscillations. Our findings demonstrated the possibility of using DPSCs and SHEDs as an autologous stem cell-based therapy for sensorineural hearing loss patients. K E Y W O R D S auditory, brainstem slice (ABS), deciduous teeth (SHED), human dental pulp stem cell (DPSC), spiral ganglion neuron (SGN), stem cell from human exfoliated

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Functional Stem Cell Integration into Neural Networks Assessed by Organotypic Slice Cultures

Cited by 7 publications

References 111 publications

Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation

Three-Dimensional Graphene Enhances Neural Stem Cell Proliferation Through Metabolic Regulation

Astrocytes release prostaglandin E2 to modify respiratory network activity

Neuronal differentiation of dental pulp stem cells from human permanent and deciduous teeth following coculture with rat auditory brainstem slices

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