Dynamic changes in connexin expression following engraftment of neural stem cells to striatal tissue

Jäderstad, Johan; Jäderstad, Linda Maria; Herlenius, Eric

doi:10.1016/j.yexcr.2010.07.011

Cited by 14 publications

(24 citation statements)

References 185 publications

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“…What is more important, immunohistochemical staining and dye-transfer studies revealed that the NSCs already formed functional gap junctions prior to engraftment, thereby creating a substrate for subsequent graft and host communication. In an organotypic ex vivo model system for striatal tissue, at 0 and 7 d 74-94% of grafted NSCs and NSCs-derived cells expressed Cx43, suggesting a window of opportunity for successful host cell rescue by NSC transplantation [26]. …”

Section: Discussionmentioning

confidence: 99%

Experimental research Enhanced connexin 43 expression following neural stem cell transplantation in a rat model of traumatic brain injury

Ma²,

Kong³

et al. 2013

aoms

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IntroductionReestablishment of functional networks after traumatic brain injury (TBI) has been proffered as one of the goals of neural stem cell (NSC) transplantation therapeutics. Gap junctions provide essential means for direct cellular communication by transferring small molecules and ions, which may provide insights into the interplay between grafted NSCs and host cells.Material and methodsThirty-six adult male Wister rats were used in this study. The controlled cortical impact (CCI) model of brain injury has been performed. Seventy-two hours after CCI injury, animals were randomly assigned to two groups: PBS- and NSC- transplanted group. NSCs-transplanted group received delivery of the NSCs suspension to the cortex below the injury cavity in the ipsilateral hemisphere. At 1, 2, and 4 weeks post-transplantation, we investigated the expression patterns of gap junction-associated connexin 43 (Cx43) in the transplant site and the border of CCI by immunohistochemistry, Western blot and RT-PCR.ResultsOur findings showed that Cx43 staining was significantly greater in the transplant site and the border of CCI in the NSCs-transplanted rats compared to the control rats at different time points (p < 0.01 at 1 week, p < 0.05 at 2 and 4 weeks). Significantly higher gene and protein expression of Cx43 was found in NSCs-transplanted rats compared to the control rats in the period of 4 weeks post-transplantation (p < 0.01), and remained at a higher level until 2 weeks with or without NSC transplantation.ConclusionsIt is proposed that gap junction-associated Cx43 might participate in NSCs’ beneficial effects via gap-junctional coupling by which grafted NSCs integrate into host neural tissue following transplantation after TBI.

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Section: Discussionmentioning

confidence: 99%

Experimental research Enhanced connexin 43 expression following neural stem cell transplantation in a rat model of traumatic brain injury

Ma²,

Kong³

et al. 2013

aoms

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“…GJIC increases in the CNS after hypoxia and ischemia (205). It is an important link between grafted neural stem cells (NSCs) and host cells after NSC engraftment (291;292). Recently, a study shows that murine NSCs (C17.2) subjected to hypoxic preconditioning before the engraftment increase Cx43 expression and improve subsequent graft and host communication (293).…”

Section: Connexins Gap Junctions and Hemichannels In Stem Cell Dementioning

confidence: 99%

Biological role of connexin intercellular channels and hemichannels

Kar

Batra

Riquelme

et al. 2012

Archives of Biochemistry and Biophysics

194

165

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“…GJIC between grafted neural stem (NS) cells and brain cells [33] appears to be an essential participant in the neuroprotective effect associated with NS cell engraftment, particularly at the connexin-associated gap junction interface. Utilizing NS cells grafted into an ex vivo model system for striatal tissue, Jäderstad et al [66] found that CX43 expression transiently peaked in host cells following traumatic stimulation, suggesting a window of opportunity for NS cells to establish gap junctions with the host tissue and rescue the damaged cells. Since AF cells express high levels of CX43 and form functional gap junctions, they have the capacity to mimic a similar connexin-mediated rescue during this critical time frame.…”

Section: Discussionmentioning

confidence: 99%

Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells

Jezierski

Rennie

Tremblay

et al. 2012

Stem Cells International

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The usage of stem cells is a promising strategy for the repair of damaged tissue in the injured brain. Recently, amniotic fluid (AF) cells have received a lot of attention as an alternative source of stem cells for cell-based therapies. However, the success of this approach relies significantly on proper interactions between graft and host tissue. In particular, the reestablishment of functional brain networks requires formation of gap junctions, as a key step to provide sufficient intercellular communication. In this study, we show that AF cells express high levels of CX43 (GJA1) and are able to establish functional gap junctions with cortical cultures. Furthermore, we report an induction of Cx43 expression in astrocytes following injury to the mouse motor cortex and demonstrate for the first time CX43 expression at the interface between implanted AF cells and host brain cells. These findings suggest that CX43-mediated intercellular communication between AF cells and cortical astrocytes may contribute to the reconstruction of damaged tissue by mediating modulatory, homeostatic, and protective factors in the injured brain and hence warrants further investigation.

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Dynamic changes in connexin expression following engraftment of neural stem cells to striatal tissue

Cited by 14 publications

References 185 publications

Experimental research Enhanced connexin 43 expression following neural stem cell transplantation in a rat model of traumatic brain injury

Experimental research Enhanced connexin 43 expression following neural stem cell transplantation in a rat model of traumatic brain injury

Biological role of connexin intercellular channels and hemichannels

Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells

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