Commissioning of the 36 T Series-Connected Hybrid Magnet at the NHMFL

Bird, M.D.; Brey, Wallace S.; Cross, Timothy A.; Dixon, I.R.; Griffin, Adrian; Hannahs, S. T.; Kynoch, John; Litvak, Ilya M.; Schiano, Jefferey L.; Toth, J.

doi:10.1109/tasc.2017.2781727

Cited by 11 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The resulting sample was cycled between 42

C and room temperature ten times. Spectra were taken at the National High Magnetic Field Lab (Tallahassee, FL, USA) using the 40 mm bore Series Connected Hybrid (SCH) magnet system, currently the highest-field NMR magnet [ 38 ]. Two-dimensional

C-

C cross polarization [ 39 ] dipolar assisted rotational resonance [ 40 ] (CP DARR) spectra were obtained at 36 T, with a 2 mm CPMAS probe tuned to frequencies of

N, with ferroshims, at a temperature of 10

C, a 100 ms mixing time, and a MAS rate of 24.4 kHz [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

The Droserasin 1 PSI: A Membrane-Interacting Antimicrobial Peptide from the Carnivorous Plant Drosera capensis

et al. 2020

View full text Add to dashboard Cite

The Droserasins, aspartic proteases from the carnivorous plant Drosera capensis, contain a 100-residue plant-specific insert (PSI) that is post-translationally cleaved and independently acts as an antimicrobial peptide. PSIs are of interest not only for their inhibition of microbial growth, but also because they modify the size of lipid vesicles and strongly interact with biological membranes. PSIs may therefore be useful for modulating lipid systems in NMR studies of membrane proteins. Here we present the expression and biophysical characterization of the Droserasin 1 PSI (D1 PSI.) This peptide is monomeric in solution and maintains its primarily α -helical secondary structure over a wide range of temperatures and pH values, even under conditions where its three disulfide bonds are reduced. Vesicle fusion assays indicate that the D1 PSI strongly interacts with bacterial and fungal lipids at pH 5 and lower, consistent with the physiological pH of D. capensis mucilage. It binds lipids with a variety of head groups, highlighting its versatility as a potential stabilizer for lipid nanodiscs. Solid-state NMR spectra collected at a field strength of 36 T, using a unique series-connected hybrid magnet, indicate that the peptide is folded and strongly bound to the membrane. Molecular dynamics simulations indicate that the peptide is stable as either a monomer or a dimer in a lipid bilayer. Both the monomer and the dimer allow the passage of water through the membrane, albeit at different rates.

show abstract

“…The resulting sample was cycled between 42

C-

C cross polarization [ 39 ] dipolar assisted rotational resonance [ 40 ] (CP DARR) spectra were obtained at 36 T, with a 2 mm CPMAS probe tuned to frequencies of

N, with ferroshims, at a temperature of 10

C, a 100 ms mixing time, and a MAS rate of 24.4 kHz [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

The Droserasin 1 PSI: A Membrane-Interacting Antimicrobial Peptide from the Carnivorous Plant Drosera capensis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Unlike superconducting magnets for which ramp time can be days or even weeks, the SCH magnet ramps up to maximum field in about a half-hour [21]. For resistive magnets like the Keck, ramp time is typically several minutes.…”

Section: Instrumentation Shim Systemsmentioning

confidence: 99%

“…A number of Bitter-type powered resistive magnets with improved homogeneity have been built at the NHMFL to be used for high demanding applications such as magnetic resonance. The series-connected hybrid (SCH) magnet with superconducting outsert and Florida-Bitter type resistive insert reached 36.1 T field in November 2016 [21,22]. Prior to the completion of the SCH magnet, NHMFL users could choose between two resistive magnets with higher field uniformity than a standard high field magnet.…”

Section: Introductionmentioning

confidence: 99%

Achieving 1 ppm field homogeneity above 24 T: Application of differential mapping for shimming Keck and the Series Connected Hybrid magnets at the NHMFL

Litvak

Griffin

Paulino

et al. 2019

Journal of Magnetic Resonance

Self Cite

View full text Add to dashboard Cite

Powered resistive and resistive-superconductive hybrid magnets can reach fields higher than superconducting NMR magnets but lack the field homogeneity and temporal stability needed for high resolution NMR. Due to field fluctuations in powered magnets, commercially available mapping systems fail to produce maps of these magnets with sufficient reproducibility, thus hampering attempts to improve homogeneity of the field they generate. Starting with a commercial mapper, we built a mapping system which uses a two-channel (measurement + reference) mapper probe. We used this system to map and then to shim two magnets of Florida Bitter type at the National High Magnetic Field Laboratory in Tallahassee, FL. With a combination of passive (ferromagnetic) and active shims we achieved 2.3 ppm homogeneity in 1 cm diameter spherical volume (dsv) at 25.0 T in the Keck resistive magnet, and 0.9 ppm homogeneity in 1 cm dsv at 23.5, 28.2, and 35.2 T in the series-connected resistive-superconductive hybrid (SCH) magnet.

show abstract

Solid-state NMR of quadrupolar nuclei: Selected new methods and applications

Bryce

2022

Annual Reports on NMR Spectroscopy

View full text Add to dashboard Cite

Commissioning of the 36 T Series-Connected Hybrid Magnet at the NHMFL

Cited by 11 publications

References 14 publications

The Droserasin 1 PSI: A Membrane-Interacting Antimicrobial Peptide from the Carnivorous Plant Drosera capensis

The Droserasin 1 PSI: A Membrane-Interacting Antimicrobial Peptide from the Carnivorous Plant Drosera capensis

Achieving 1 ppm field homogeneity above 24 T: Application of differential mapping for shimming Keck and the Series Connected Hybrid magnets at the NHMFL

Solid-state NMR of quadrupolar nuclei: Selected new methods and applications

Contact Info

Product

Resources

About

Commissioning of the 36 T Series-Connected Hybrid Magnet at the NHMFL

Cited by 11 publications

References 14 publications

The Droserasin 1 PSI: A Membrane-Interacting Antimicrobial Peptide from the Carnivorous Plant Drosera capensis

The Droserasin 1 PSI: A Membrane-Interacting Antimicrobial Peptide from the Carnivorous Plant Drosera capensis

Achieving 1 ppm field homogeneity above 24 T: Application of differential mapping for shimming Keck and the Series Connected Hybrid magnets at the NHMFL

Solid-state NMR of quadrupolar nuclei: Selected new methods and applications

Contact Info

Product

Resources

About

Achieving 1 ppm field homogeneity above 24 T: Application of differential mapping for shimming Keck and the Series Connected Hybrid magnets at the NHMFL