Commercial development and introduction of DiTera<sup>TM</sup>, a new nematicide

Warrior, Prem; Rehberger, Linda A; Beach, Mark R.; Grau, Philip A; Kirfman, G.W.; Conley, James

doi:10.1002/(sici)1096-9063(199903)55:3<376::aid-ps918>3.0.co;2-s

Cited by 36 publications

(19 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This approach has already been employed with the fungus Myrothecium, which was originally isolated from Heterodera glycines Ichinohe (soybean cyst nematode, SCN). Activity of culture products from the fungus includes nematicidal effects on adult nematodes, alteration of rhizosphere ecology and inhibition of egg hatch, juvenile development and root-nding (Warrior et al, 1999;Perry et al, 2000;Twomey et al, 2000Twomey et al, , 2002Fernández et al, 2001). The fungus is now cultured to generate the novel nematicides that are utilised in the product DiTera ® (Valent BioSciences Corporation, Libertyville, IL, USA).…”

mentioning

confidence: 99%

Activity of fungal culture filtrates against soybean cyst nematode and root-knot nematode egg hatch and juvenile motility

Meyer¹,

Huettel²,

Liu³

et al. 2004

Nematol

View full text Add to dashboard Cite

Fungi were isolated from soybean cyst nematode (SCN, Heterodera glycines) eggs collected in China, and 253 fungal isolates were assayed for production of compounds active against SCN and root-knot nematode (RKN, Meloidogyne incognita). Fungal isolates were grown for 3 and 7 days in potato dextrose broth (PDB), the culture broths were sterile-ltered to remove fungal biomass, and the ltrates were placed into 24-well plates to test for effects on egg hatch and juvenile motility. Meloidogyne incognita egg hatch ranged from 2 to 121% of hatch in PDB controls and H. glycines hatch from 15 to 224%. Activities of ltrates harvested after 3 and 7 days were signi cantly correlated. Only four isolates produced ltrates that signi cantly inhibited juvenile motility of SCN, RKN or both nematodes. This study identi ed fungal isolates capable of producing compounds active against these nematodes, and demonstrated that there was a low correlation in activity against SCN and RKN. The active fungal isolates are candidates for studies on identi cation of potential nematicides.

show abstract

mentioning

confidence: 99%

Activity of fungal culture filtrates against soybean cyst nematode and root-knot nematode egg hatch and juvenile motility

Meyer¹,

Huettel²,

Liu³

et al. 2004

Nematol

View full text Add to dashboard Cite

show abstract

“…The fungus is fermented in liquid nutrient media and spray-dried. The drying process kills the fungus but all the fermentation products are retained (Warrior et al, 1999). The product contains both nutrients, which may * Corresponding author, e-mail: kospence@ucdavis.edu assist plants in growing through nematode infection, and fungal metabolites, which may have a range of effects on the nematode, the plant and the nematode-plant interaction.…”

mentioning

confidence: 99%

Biopesticides with complex modes of action: direct and indirect effects of DiTera® on Meloidogyne incognita

Spence

Lewis

2010

Nematol

View full text Add to dashboard Cite

Bionematicides may exhibit complex modes of action based on: i) direct lethal or sub-lethal effects on the nematode; ii) promotion of plant growth and damage tolerance; and/or iii) influencing the interaction between the plant and the nematode by inducing plant defences. A suite of assays was conducted to characterise the mode of action of the commercial bionematicide DiTera ® (Valent Biosciences, Chicago, IL, USA). Agar-based behavioural assays revealed significant chemotactic responses of second-stage juveniles of Meloidogyne incognita to DiTera with concentrations of 1.0% and 0.1% being repellent and 0.01% being attractive to M. incognita. In addition, exposure to 1.0% DiTera reduced the velocity and increased the meander of nematodes in no-choice tests. These experiments were complemented with soil-based assays. Rhizotron and minirhizotron assays that allowed spatially explicit evaluation of root growth demonstrated root proliferation of Solanum lycopersicum cv. Rutgers in response to localised applications of DiTera. Our results confirm that DiTera can positively affect plant growth and may influence nematode foraging behaviour. However, results of the soil-based assays provided little support for the hypothesis that DiTera has direct or indirect (plant-mediated) effects on M. incognita performance. This is in contrast to some of the published literature, and it may be that the chosen methods were not appropriate to detect these effects.

show abstract

“…This product was used for control of root knot nematodes [Meloidogyna incognita (Kof & White) Chitwood]. Based on acute toxicology batteries, this product and its formulations were classified as US-EPA Category III (slightly toxic) and Category IV (practically non-toxic) pesticides (Warrior et al 1999).…”

mentioning

confidence: 99%

Submerged culture of a mycelial formulation of a bioherbicidal strain of Myrothecium verrucaria with mitigated mycotoxin production

Boyette

Weaver

Hoagland

et al. 2008

World J Microbiol Biotechnol

View full text Add to dashboard Cite

A mycelial formulation of the fungus Myrothecium verrucaria (IMI 361690) containing 0.20% Silwet L-77 surfactant was found to be highly efficacious in controlling the exotic invasive weed kudzu. The mycelium can be rapidly (48-72 h) produced in several media, including an inexpensive soy flour-corn meal medium. Mycelial yields were 2, 10, and 25 g dry weight l -1 in Czapek-Dox, Richard's V-8, and soy flour-corn meal media, respectively. Scale-up production in soy flour-corn meal medium using laboratory fermenters (10-25 l), resulted in a mycelial formulation that caused 90% mortality of naturally-occurring mature (0.9-1.0 m in height) kudzu within 48 h after application in field experiments. HPLC analyses revealed that the mycelium produced in this liquid culture contained no detectable amounts of the trichothecene mycotoxins roridin A and verrucarin A (limit of detection 2 lg ml -1 ). This has resulted in a safer, yet effective bioherbicidal product. We anticipate that these findings should improve the probability of EPA registration and subsequent commercial development of this bioherbicide.

show abstract

Commercial development and introduction of DiTera^TM, a new nematicide

Abstract: Figure 3. ELSD and total ion control of a mixture with known compound from Table 1.

Cited by 36 publications

References 1 publication

Activity of fungal culture filtrates against soybean cyst nematode and root-knot nematode egg hatch and juvenile motility

Activity of fungal culture filtrates against soybean cyst nematode and root-knot nematode egg hatch and juvenile motility

Biopesticides with complex modes of action: direct and indirect effects of DiTera® on Meloidogyne incognita

Submerged culture of a mycelial formulation of a bioherbicidal strain of Myrothecium verrucaria with mitigated mycotoxin production

Contact Info

Product

Resources

About

Commercial development and introduction of DiTeraTM, a new nematicide

Abstract: Figure 3. ELSD and total ion control of a mixture with known compound from Table 1.

Cited by 36 publications

References 1 publication

Activity of fungal culture filtrates against soybean cyst nematode and root-knot nematode egg hatch and juvenile motility

Activity of fungal culture filtrates against soybean cyst nematode and root-knot nematode egg hatch and juvenile motility

Biopesticides with complex modes of action: direct and indirect effects of DiTera® on Meloidogyne incognita

Submerged culture of a mycelial formulation of a bioherbicidal strain of Myrothecium verrucaria with mitigated mycotoxin production

Contact Info

Product

Resources

About

Commercial development and introduction of DiTera^TM, a new nematicide