Commercial and emerging technologies for cancer diagnosis and prognosis based on circulating tumor exosomes

Ohannesian, Nareg; Gunawardhana, Loku; Misbah, Ibrahim; Rakhshandehroo, Mohsen; Lin, Steven H.; Shih, Wei Chuan

doi:10.1088/2515-7647/ab8699

Cited by 9 publications

(11 citation statements)

References 89 publications

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“…PANORAMA would provide new capabilities in label-free imaging and single nanoparticle analysis. More importantly, molecular imaging can be envisioned with a functionalized AGNIS surface for single biological nanoparticle analysis including extracellular vesicles such as exosomes [31] and viruses.…”

Section: Discussionmentioning

confidence: 99%

Plasmonic nano-aperture label-free imaging (PANORAMA)

et al. 2020

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Label-free optical imaging of nanoscale objects faces fundamental challenges. Techniques based on propagating surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR) have shown promises. However, challenges remain to achieve diffraction-limited resolution and better surface localization in SPR imaging. LSPR imaging with dark-field microscopy on metallic nanostructures suffers from low light throughput and insufficient imaging capacity. Here we show ultra-near-field index modulated PlAsmonic NanO-apeRture lAbel-free iMAging (PANORAMA) which uniquely relies on unscattered light to detect sub-100 nm dielectric nanoparticles. PANORAMA provides diffraction-limited resolution, higher surface sensitivity, and wide-field imaging with dense spatial sampling. Its system is identical to a standard bright-field microscope with a lamp and a camera – no laser or interferometry is needed. In a parallel fashion, PANORAMA can detect, count and size individual dielectric nanoparticles beyond 25 nm, and dynamically monitor their distance to the plasmonic surface at millisecond timescale.

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Section: Discussionmentioning

confidence: 99%

Plasmonic nano-aperture label-free imaging (PANORAMA)

et al. 2020

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“…Exosomes are extracellular vesicles known for carrying abundant dsDNA, mRNA, and miRNA of the source cells. 46 Their size ranges from 50 to 250 nm and are excreted by every mammalian cell. Recent evidence suggests that dysregulation of the genetic content within exosomes has a major role in tumor progression and in the surrounding microenvironment.…”

Section: Resultsmentioning

confidence: 99%

“…coli, which ranges between 5 and 10 μm, we divert our attention toward collection of biological organisms that are in nanoscale. Exosomes are extracellular vesicles known for carrying abundant dsDNA, mRNA, and miRNA of the source cells . Their size ranges from 50 to 250 nm and are excreted by every mammalian cell.…”

Section: Resultsmentioning

confidence: 99%

Directed Concentrating of Micro-/Nanoparticles via Near-Infrared Laser Generated Plasmonic Microbubbles

Ohannesian

Misbah

et al. 2020

ACS Omega

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Directed concentrating of micro- and nanoparticles via laser-generated plasmonic microbubbles in a liquid environment is an emerging technology. For effective heating, visible light has been primarily employed in existing demonstrations. In this paper, we demonstrate a new plasmonic platform based on nanoporous gold disk (NPGD) array. Thanks to the highly tunable localized surface plasmon resonance of the NPGD array, microbubbles of controlled size can be generated by near-infrared (NIR) light. Using NIR light provides several key advantages over visible light in less interference with standard microscopy and fluorescence imaging, preventing fluorescence photobleaching, less susceptible to absorption and scattering in turbid biological media, and much reduced photochemistry, phototoxicity, and so forth. The large surface-to-volume ratio of NPGD further facilitates the heat transfer from these gold nanoheaters to the surroundings. While the microbubble is formed, the surrounding liquid circulates and direct microparticles randomly dispersed in the liquid to the bottom NPGD surface, which can be made to yield a unique collection of 3D hollow dome microstructures with bubbles larger than 5 μm. Such capability can also be employed in concentrating suspended colloidal nanoparticles at desirable sites and with the preferred configuration enhancing the sensor performance. Specifically, the interaction among concentrated nanoparticles and their interactions with the underlying substrate have been investigated for the first time. These collections have been characterized using optical microscopy, scanning electron microscopy, hyperspectral localized surface plasmon resonance imaging, and hyperspectral Raman imaging. In addition to various micro- and nanoparticles, the plasmonic microbubbles are also shown to collect biological cells and extracellular nanovesicles such as exosomes. By using a spatial light modulator to project the laser in arbitrary patterns, parallel concentrating can be achieved to fabricate an array of clusters.

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“…Therefore researchers have recently started integrating different methods based on the physical properties of exosomes with the design of the microfluidic device. Thus, microfluidics-based isolation techniques have developed and evolved over the years [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. The following sections will discuss some of the most interesting methods mostly based on the immunoaffinity approaches.…”

Section: Isolation and Detection Techniquesmentioning

confidence: 99%

Microfluidic Platforms for the Isolation and Detection of Exosomes: A Brief Review

et al. 2022

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Extracellular vesicles (EVs) are a group of communication organelles enclosed by a phospholipid bilayer, secreted by all types of cells. The size of these vesicles ranges from 30 to 1000 nm, and they contain a myriad of compounds such as RNA, DNA, proteins, and lipids from their origin cells, offering a good source of biomarkers. Exosomes (30 to 100 nm) are a subset of EVs, and their importance in future medicine is beyond any doubt. However, the lack of efficient isolation and detection techniques hinders their practical applications as biomarkers. Versatile and cutting-edge platforms are required to detect and isolate exosomes selectively for further clinical analysis. This review paper focuses on lab-on-chip devices for capturing, detecting, and isolating extracellular vesicles. The first part of the paper discusses the main characteristics of different cell-derived vesicles, EV functions, and their clinical applications. In the second part, various microfluidic platforms suitable for the isolation and detection of exosomes are described, and their performance in terms of yield, sensitivity, and time of analysis is discussed.

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Commercial and emerging technologies for cancer diagnosis and prognosis based on circulating tumor exosomes

Cited by 9 publications

References 89 publications

Plasmonic nano-aperture label-free imaging (PANORAMA)

Plasmonic nano-aperture label-free imaging (PANORAMA)

Directed Concentrating of Micro-/Nanoparticles via Near-Infrared Laser Generated Plasmonic Microbubbles

Microfluidic Platforms for the Isolation and Detection of Exosomes: A Brief Review

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