“Command” surfaces with thermo-switchable antibacterial activity

Non-cytotoxic, temperature-responsive and antibacterial POEGMA based nanocomposite coatings with silver nanoparticles

“…Subsequently, Ag ions were adsorbed into the polymer coating and reduced to AgNPs by sodium borohydride. 29,30 It is important to note, that the "freshness" of the sodium borohydride is of a crucial impact on the amount of the synthesized AgNPs. Therefore, only freshly opened reagent was used here.…”

“…In our previous work 30 we have demonstrated the temperatureswitched killing of the bacteria on POEGMA188 and P4VP based coatings where no signicant difference was observed at 4 C between the amounts of bacteria counted on the nanocomposite coatings with AgNPs and on the "bare" coating control sample. In contrast, at 37 C almost no bacteria were visible for temperature-responsive coating with AgNPs, whereas the growth of bacteria remained unperturbed for "pure" coating, indicating well-expressed temperature-dependent antibacterial properties of AgNPs integrated into brushes.…”

“…4 demonstrates cultivation of E. coli and S. aureus model bacterial strains on the Petri plates aer incubation with the POEGMA188-based nanocomposites as well as POEGMA188 control samples at 4 C (le panel) and 37 C (right panel) that conrms our previous results. 30 Although AgNPs have astounding pharmacological properties, the possibility of their application is strongly limited by their potential cytotoxicity and genotoxicity, activated by various mechanisms, in particular the production of excess reactive oxygen species (ROS), which results in oxidative stress, reduced levels of glutathione, elevated lipid peroxidation, inammation, DNA damage, altered cell cycle and proliferation capacity, and apoptosis and necrosis. 33,[44][45][46] AgNPs have also the tendency to accumulate in human organs, like liver, spleen, lungs, and kidney 7,8,31 and they are able to can cross the blood-brain barrier (BBB) and accumulate in different regions of the brain.…”

“…In our previous works we have described a new method of fabrication of the "command" antibacterial surfaces with temperature-switched killing of the bacteria. 29,30 The coatings were based on silver nanoparticles (AgNPs) embedded in temperature-responsive poly(di(ethylene glycol)methyl ether methacrylate) -(POEGMA188) as well as in poly(4-vinylpyridine) -(P4VP) coatings attached to a glass surface. Detailed analysis of their properties, performed using numerous surface-sensitive techniques, showed strong impact of the chemical nature of the graed polymer brushes on the shape of the silver nanoparticles.…”

“…Temperature-depended antibacteriality was demonstrated here for model bacterial strains (S. aureus and E. coli), con-rming our previous results. 30 Additionally, the impact of nanocomposite coatings on mammalian cells was examined. For this purpose proliferation of healthy and cancerous cells was analyzed for two cell lines.…”