Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus

Abstract

ObjectiveWe assessed the therapeutic effects of photobiomodulation (PBM) and adipose-derived stem cell (ADS) treatments individually and together on the maturation step of repairing of a delayed healing wound model in rats with type 1 diabetes mellitus (DM1).Research design and methodsWe randomly assigned 24 rats with DM1 to four groups (n=6 per group). Group 1 was the control (placebo) group. In group 2, allograft human ADSs were transplanted. Group 3 was subjected to PBM (wavelength: 890 nm, peak power output: 80 W, pulse frequency: 80 Hz, pulsed duration: 180 ns, duration of exposure for each point: 200 s, power density: 0.001 W/cm2, energy density: 0.2 J/cm2) immediately after surgery, which continued for 6 days per week for 16 days. Group 4 received both the human ADS and PBM. In addition, we inflicted an ischemic, delayed healing, and infected wound simulation in all of the rats. The wounds were infected with methicillin-resistant Staphylococcus aureus (MRSA).ResultsAll three treatment regimens significantly decreased the amount of microbial flora, significantly increased wound strength and significantly modulated inflammatory response and significantly increased angiogenesis on day 16. Microbiological analysis showed that PBM+ADS was significantly better than PBM and ADS alone. In terms of wound closure rate and angiogenesis, PBM+ADS was significantly better than the PBM, ADS and control groups.ConclusionsCombination therapy of PBM+ADS is more effective that either PBM or ADS in stimulating skin injury repair, and modulating inflammatory response in an MRSA-infected wound model of rats with DM1.

SARS-CoV-2 infection in patients with diabetes mellitus and hypertension: a systematic review

“…However, T2D is developed when the body cannot properly respond to insulin. The most common type of diabetes is T2D, which consists about 85% to 95% of diabetic cases throughout the world (Ebrahimpour-Malekshah et al, 2020;Kulcsar et al, 2019). Etiologically, T2D is highly linked to obesity as well as the outcomes of chronic inflammation caused by excess adipose tissue.…”

In Vitro Wound Healing Potential of Photobiomodulation Is Possibly Mediated by Its Stimulatory Effect on AKT Expression in Adipose‐Derived Stem Cells

“…Our results also showed a significant increase in cell viability, corresponding to the results observed in a similar study by de Villiers et al [ 31 ], who observed an increase in cell viability in isolated ADSCs and a commercial ADSC cell line irradiated at a wavelength of 636 nm and a fluence of 5 J/cm 2 . Ebrahimpour-Malekshah et al irradiated diabetic mice with an infected (MRSA) ischemic wound with or without transplanted ADSCs at 890 nm (power 80 W; pulse frequency 80 Hz; pulsed duration 180 ns; duration of exposure 200 s; power density 0.001 W/cm 2 ; and energy density 0.2 J/cm 2 ) 6 days per week for 16 days [ 39 ]. There was a significant decrease in microbial flora and inflammatory response and a significant increase in wound closure rate and strength and angiogenesis in all treated mice; however, it was found that PBM together with transplanted ADSCs showed superior results as compared to PBM and ADSC transplantation alone [ 39 ].…”

Transplantation of photobiomodulation-preconditioned diabetic stem cells accelerates ischemic wound healing in diabetic rats

“…Recently, in our lab, we have engrafted healthy AD-MSC cells into wounds of diabetic rats [23,26]. However, in the current probe, we engrafted preconditioned diabetic AD-MSC with PBM into wounds of diabetic rats.…”

“…We photographed the wounds on days 0, 4, 8, 12, and 16 and measured the wound surface area by ImageJ software (NIH, USA). The wound closure rate was calculated as follows [26]. Â Surfacearea at day 0 − surfacearea at day X ð Þ = surfacearea at day 0 Â 100%:…”