Combined Strategy of Mesenchymal Stem Cell Injection With Vascular Endothelial Growth Factor Gene Therapy for the Treatment of Diabetes‐Associated Erectile Dysfunction

Qiu, Xuefeng; Sun, Chao; Yao, Wen; Lin, Haocheng; Sun, Zhaorui; Chen, Yun; Wang, Run; Dai, Yutian

doi:10.2164/jandrol.110.012666

Cited by 84 publications

(77 citation statements)

References 42 publications

(45 reference statements)

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“…Some studies confirmed that embryonic stem cells can be used for the treatment of neurogenic ED (Eglitis and Mezey 1997). Studies of the effect of mesenchymal (Bivalacqua et al 2007;Qiu et al 2012), human neural crest (Song et al 2008), and adipose tissue-derived stem cells (Albersen et al 2010;Garcia et al 2010;Huang et al 2010) on ED treatment have also been performed by other researchers using intracavernous injection of these cells in rats. The results indicated that ED could be improved to a certain extent when treated with these cells.…”

Section: Introductionmentioning

confidence: 96%

Isolation and passage of muscle-derived stem cells from the rat penile corpora cavernosa and induction of differentiation into smooth muscle cells

Xue

Dong

et al. 2013

Cytotechnology

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This study treated the isolation and passage of muscle-derived stem cells (MDSCs) from rat penile corpora cavernosa, detection of stem cell marker expression, observation of their self-renewal and continuous proliferation, and demonstration of their potential to differentiate into smooth muscle cells in co-culture. Muscle-derived stem cells from the rat penile corpora cavernosa were isolated and purified. The expression of stem cell markers Sca-1 and desmin was detected in PP6 cells, thus confirming that the main components of PP6 cells are MDSCs. The expression of Sca-1 and desmin occurred both in PP6 cells and cells at passages 3, 6, and 8, and there was no significant decrease in the expression level with increasing passage number. The growth curves indicated that the cell doubling time was approximately 48 h. The cells entered the stationary phase after approximately 7 days of culture. The proliferative activity of the cells at passage 8 remained unchanged. After 2 days of co-culture with smooth muscle cells, the DAPI-labeled MDSCs tended to exhibit smooth muscle cell morphology and expression of a-SMA was detected. MDSCs exist in the rat penile corpora cavernosa and possess the potential to differentiate into smooth muscle cells. This discovery serves as the basis in view of the potential use of endogenous stem cells for the treatment of erectile dysfunction (ED).

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Section: Introductionmentioning

confidence: 96%

Isolation and passage of muscle-derived stem cells from the rat penile corpora cavernosa and induction of differentiation into smooth muscle cells

Xue

Dong

et al. 2013

Cytotechnology

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“…Because stem cells can directionally differentiate into corpus cavernosum vascular endothelial cells and smooth muscle cells under a specific microenvironment, the transplantation of stem cells for treating diabetic ED was proposed as a potential therapeutic approach [8][9][10] . Mesenchymal stem cells (MSCs) have been tested in transplantation therapy for diabetic ED [11][12][13] . Although stem cell transplantation can improve erectile function in animal studies, the effect last only 7-28 d before gradually diminishing [14][15][16] .…”

Section: Introductionmentioning

confidence: 99%

ROS activates JNK-mediated autophagy to counteract apoptosis in mouse mesenchymal stem cells in vitro

et al. 2015

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Aim: Transplantation of mesenchymal stem cells (MSCs) for the treatment of diabetic erectile dysfunction (ED) is hampered by apoptosis of the transplanted cells. In diabetic ED, there is increased oxidative stress and decreased NO in the corpora cavernosa, and reactive oxygen species (ROS) induce apoptosis of the transplanted cells. In this study we examined whether and how autophagy was involved in ROS-induced apoptosis of MSCs. Methods: Mouse C3H10 MSCs were treated with H 2 O 2 to simulate the high oxidative condition in diabetic ED. Cell viability was measured using MTT assay. Apoptosis was analyzed by flow cytometry. Apoptosis-and autophagy-related proteins were detected with Western blot assays. Intracellular autophagosome accumulation was studied using transmission electron microscopy.

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“…Actually no safety system has been inserted in the MSCs to eliminate them when excessively proliferating, no selective genetic insertion has been reported, thus increasing oncogenic risk. Moreover, no solution has been added to overcome problems like virus spread and reversion to its original form [Qiu et al 2012].…”

Section: Stem Cellsmentioning

confidence: 99%

Optimizing postoperative sexual function after radical prostatectomy

Tutolo

Briganti

Suardi

et al. 2012

Therapeutic Advances in Urology

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Erectile dysfunction (ED) is one of the complications associated with pelvic surgery. The significance of ED as a complication following pelvic surgery, especially radical prostatectomy (RP), lies in the negative impact that it has on patients' sexual and overall life. In the literature, rates of ED following RP range from 25% to 100%. Such variety is associated with pelvic dissection and conservation of neurovascular structures. Another important factor impacting on postoperative ED is the preoperative erectile function of the patient. Advances in the knowledge of pelvic anatomy and pathological mechanisms led to a refinement of pelvic surgical techniques, with attention to the main structures that if damaged compromise erectile function. These improvements resulted in lower postoperative ED rates and better erectile recovery, especially in patients undergoing RP. Furthermore, surgery alone is not sufficient to prevent this complication, and thus, several medical strategies have been tested with the aim of maximizing erectile function recovery. Indeed it seems that prevention of postoperative ED must be addressed by a multimodal approach. The aim of this review is to give a picture of recent knowledge, novel techniques and therapeutic approaches in order to reach the best combination of treatments to reduce the rate of ED after pelvic surgery.

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Combined Strategy of Mesenchymal Stem Cell Injection With Vascular Endothelial Growth Factor Gene Therapy for the Treatment of Diabetes‐Associated Erectile Dysfunction

Cited by 84 publications

References 42 publications

Isolation and passage of muscle-derived stem cells from the rat penile corpora cavernosa and induction of differentiation into smooth muscle cells

Isolation and passage of muscle-derived stem cells from the rat penile corpora cavernosa and induction of differentiation into smooth muscle cells

ROS activates JNK-mediated autophagy to counteract apoptosis in mouse mesenchymal stem cells in vitro

Optimizing postoperative sexual function after radical prostatectomy

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