Combined protective effect of vitamins C and E on cadmium induced oxidative liver injury in rats

Abstract

Our study pertains to the potential ability of vitamin C and/or vitamin E, used as nutritional supplements, to alleviate oxidative stress induced by cadmium. Male rats were randomly divided into five groups of eight each. Group I served as the controls; group II received in their drinking water CdCl 2 (200 mg/L); group III received both CdCl 2 and vitamin C (1.5 g/L of water); group IV was treated with CdCl 2 and vitamin E (400 mg/kg diet); and group V received CdCl 2 + vitamin C + vitamin E. The exposure of rats to cadmium chloride for 30 days resulted in a significant decrease in body weight gain. Cadmium treatment also produced oxidative liver injury characterized by increasing serum glucose concentration, glutamate-pyruvate transaminase (GPT), alanine aminotransaminase (GOT) and alkaline phosphatase (ALP) activities. Meanwhile cadmium supplementation decreased serum total protein and albumin in animals. In addition, liver glutathione level, catalase and glutathione peroxidase (GSH-Px) activities were diminished. With vitamin C and vitamin E administration during intoxication of cadmium, corrective effects on Cd-induced oxidative stress in the liver was observed. In conclusion, this study demonstrates that oral exposure to Cd caused reduction in LPO and antioxidant enzyme activities in rat's liver, and vitamin C or vitamin E may have partial ameliorative effects on these disturbances, whereas vitamin C and vitamin E together assured a more efficient protection of the organ against the noticed oxidative stress.

Review of polyphenol‐rich products as potential protective and therapeutic factors against cadmium hepatotoxicity

“…The ability of these compounds to act as FR and ROS scavengers is well-established and they are widely used in the prevention of many oxidative stress-related diseases such as cancer or atherosclerosis (Padayatty et al, 2003;WHO, 2003). Moreover, numerous studies in animal models confirm the protective effect of supplementation with vitamins C and E against damage to the liver caused by heavy metals (Brzóska, Borowska, & Tomczyk, 2016;El-Refaiy & Eissa, 2013;El-Sayed et al, 2016;Greń et al, 2012;Grosicki, 2004;Layachi & Kechrid, 2012;Nemmiche, Chabane-Sari, & Guiraud, 2007).…”

“…Vitamin E partially prevented this metal-caused increase in the concentrations of PC and MDA, and inhibition of the activities of CAT and SOD. Moreover, vitamin E attenuated this element-induced decrease in the hepatic concentration of GSH and decreased the activity of LDH in the liver to a value lower than in the control group(Nemmiche et al, 2007) Layachi and Kechrid (2012). have shown that supplementation with vitamins C and E together was more effective in the protection from the adverse effects of Cd in this organ than administration of these vitamins separately.…”

“…Vitamin E (α-tocopherol) is the main lipid-soluble membranebound antioxidant with the major function in the prevention against lipid peroxidation (Galli et al, 2017). Nemmiche et al (2007) Greń et al, 2012;Layachi & Kechrid, 2012;Nemmiche et al, 2007), whereas their indirect action is connected to decreasing the accumulation of this metal in the liver (Grosicki, 2004).…”

Biotoxicité hématologique du sulfate de cadmium chez les rats Wistar

“…Les lots 2, 3, 4, 5 et 6 de chaque sexe ont reçu par gavage respectivement 1/50 ième , 1/40 ième , 1/30 ième , soit1/20 ième et 1/10 ième de la DL 50 (200 mg/kg pc) de la solution de sulfate de cadmium qui sont respectivement 4; 5; 6,66 ; 10 et 20 mg/kg de poids corporel (pc). La durée de traitement des rats au sulfate de cadmium était de 30 Jours (Layachi et Kechrid, 2012). Et le volume administré était de 1ml par jour chaque matin pendant la durée de traitement.…”

The effect of diosmin against liver damage caused by cadmium in rats

“…In the treatment of these heavy metal-related intoxications, the use of a chelator for the chelation of the metals passing into the systemic circulation is often preferred (Flora, Mittal, & Mehta, 2008;Tandon et al, 2003;Xu et al, 1996). However, many antioxidant substances which are applied in order to prevent the possible liver oxidative stress are investigated within the scope of previous studies (Eşrefoglu, Gül, Dogru, Dogru, & Yürekli, 2007;García-Niño & Pedraza-Chaverrí, 2014;Layachi & Kechrid, 2012;Zafeer, Waseem, Chaudhary, & Parvez, 2012). Diosmin is also one of the known antioxidants (Firuzi et al, 2011;Pietta, 2000).…”