Combined effects of temperature and salinity on functional responses of haemocytes and survival in air of the clam Ruditapes philippinarum

Munari, Marco; Matozzo, Valerio; Marin, María Gabriella

doi:10.1016/j.fsi.2011.01.025

Cited by 51 publications

(30 citation statements)

References 35 publications

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“…In this study, the effects of pH and temperature on the NR uptake by haemocytes were assessed. This assay is faster than the phagocytosis assay, but equally responsive [32]. NR dye has been used extensively in in vitro assays to evaluate the effects of stressors on lysosomal membrane stability in bivalve haemocytes [33]–[36].…”

Section: Discussionmentioning

confidence: 99%

First Evidence of Immunomodulation in Bivalves under Seawater Acidification and Increased Temperature

et al. 2012

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Water acidification, temperature increases and changes in seawater salinity are predicted to occur in the near future. In such a global climate change (GCC) scenario, there is growing concern for the health status of both wild and farmed organisms. Bivalve molluscs, an important component of coastal marine ecosystems, are at risk. At the immunological level, the ability of an organism to maintain its immunosurveillance unaltered under adverse environmental conditions may enhance its survival capability. To our knowledge, only a few studies have investigated the effects of changing environmental parameters (as predicted in a GCC scenario) on the immune responses of bivalves. In the present study, the effects of both decreased pH values and increased temperature on the important immune parameters of two bivalve species were evaluated for the first time. The clam Chamelea gallina and the mussel Mytilus galloprovincialis, widespread along the coast of the Northwestern Adriatic Sea, were chosen as model organisms. Bivalves were exposed for 7 days to three pH values (8.1, 7.7 and 7.4) at two temperatures (22 and 28°C). Three independent experiments were carried out at salinities of 28, 34 and 40 PSU. The total haemocyte count, Neutral Red uptake, haemolymph lysozyme activity and total protein levels were measured. The results obtained demonstrated that tested experimental conditions affected significantly most of the immune parameters measured in bivalves, even if the variation pattern of haemocyte responses was not always linear. Between the two species, C. gallina appeared more vulnerable to changing pH and temperature than M. galloprovincialis. Overall, this study demonstrated that climate changes can strongly affect haemocyte functionality in bivalves. However, further studies are needed to clarify better the mechanisms of action of changing environmental parameters, both individually and in combination, on bivalve haemocytes.

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Section: Discussionmentioning

confidence: 99%

First Evidence of Immunomodulation in Bivalves under Seawater Acidification and Increased Temperature

et al. 2012

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“…Two-way ANOVA was used to compare the metabolite concentrations by a post-hoc test for pairwise comparisons (e.g., for comparison between two arsenic-blank and arsenic-treated groups under each salinity, or vice versa) (Munari et al, 2011). All metabolite concentrations are expressed as means7 standard deviation.…”

Section: Discussionmentioning

confidence: 99%

A metabolomic investigation on arsenic-induced toxicological effects in the clam Ruditapes philippinarum under different salinities

Zhang

Wang

et al. 2013

Ecotoxicology and Environmental Safety

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“…Similarly, salinity is a limiting factor in the distribution of aquatic organisms, and it can affect the physiological processes of estuarine organisms, such as survival, hemolymph osmolarity, tissue water content and have other sublethal effects (De Lisle and Roberts 1988; Matsuda et al 2008; Taware et al 2012; McFarland et al 2013). The importance of studying the combined effects of temperature and salinity to aquatic organisms has also been increasingly highlighted (Gagnaire et al 2006; Munari et al 2011). While there is substantial information available on the effect of single parameters on the metabolism and physiological processes of aquatic organisms, published reports on the combined effects of multiple parameters on aquatic animals, especially estuarine organisms, are still limited.…”

Section: Introductionmentioning

confidence: 99%

Effects of temperature and salinity on metabolic rate of the Asiatic clam Corbicula fluminea (Müller, 1774)

Xiao

et al. 2014

SpringerPlus

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The effects of temperature and salinity on the metabolism of the Asiatic clam Corbicula fluminea (mollusca, Lamellibranchia) were studied experimentally. Firstly, three indexes of basal metabolism (oxygen consumption rate, OCR; ammonia excretion rate, AER; and CO2 emission rate, CER), patterns of diurnal rhythm and O: N ratios were measured for three size ranges (large: h = 25.54 ± 1.96 mm, medium: h = 22.07 ± 1.33 mm and small: h = 17.70 ± 1.43 mm) at two salinities (0.3‰ and 1.8‰). The results showed that: (1) three indexes decreased with increasing body size. (2) no significant difference was found between two salinities for the O: N ratios of the small and large size, but a significant difference was found for the medium-sized one; (3) however, there were similar and distinct diurnal rhythms of metabolic rate at two salinities over a 24 hour period in three size C. fluminea.OCR, AER, CER, O: N ratios and Q10 (temperature coefficient) of small-sized C. fluminea were measured across five water temperatures (4, 11, 18, 25 and 32°C) and two salinities (0.3‰ and 1.8‰) in the following experiments. Our results of the small C. fluminea were as follows: there was no significant difference in the O: N ratios among the five temperatures and two salinity treatments; and no significant difference of three indexes between both salinity levels were observed at same temperature controlled; and three indexes increased significantly with increasing temperature from 4°C to 25°C, while no significant difference was observed in the 25-32°C range; and the highest Q10 coefficients (Q10 = 1.825 at salinity of 0.3‰ and Q10 = 1.683 at salinity of 1.8‰) were observed at the 18-25°C temperature increase, and the low values were found in the 4-11°C, 11-18°C and 25-32°C interval. It indicates that there is not a synergetic effect of our temperature and salinity on the metabolic rate of small C. fluminea, and a temperature of 18-25°C may represent an optimum adequate metabolic temperature range. For the purposes of ecological monitoring and restoration, small individuals of C. fluminea planted are more likely to survive than larger ones.

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Combined effects of temperature and salinity on functional responses of haemocytes and survival in air of the clam Ruditapes philippinarum

Cited by 51 publications

References 35 publications

First Evidence of Immunomodulation in Bivalves under Seawater Acidification and Increased Temperature

First Evidence of Immunomodulation in Bivalves under Seawater Acidification and Increased Temperature

A metabolomic investigation on arsenic-induced toxicological effects in the clam Ruditapes philippinarum under different salinities

Effects of temperature and salinity on metabolic rate of the Asiatic clam Corbicula fluminea (Müller, 1774)

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