Combined effects of irradiance, temperature and nitrate concentration on phycoerythrin content in the microalga Rhodomonas sp. (Cryptophyceae)

Chaloub, Ricardo M.; Motta, Nathania Maria S.; Araujo, Silvia P. de; Aguiar, Paula Fernandes de; Silva, Anita F. da

doi:10.1016/j.algal.2015.01.008

Cited by 57 publications

(46 citation statements)

References 33 publications

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“…A study on a species of Rhodomonas (now Pyrenomonas ) by Chaloub et al. () showed similarly that the amount of phycoerythrin increased when cells were grown at low light levels (<15 μmol photons · m −2 · s −1 ) compared to 50 and 150 μmol photons · m −2 · s −1 . In addition, Chaloub et al.…”

Section: Discussionmentioning

confidence: 96%

“…In addition, Chaloub et al. () showed that the phycoerythrin content decreased in Rhodomonas cells approaching the stationary phase. Color changes have also been observed in other cryptophytes.…”

Section: Discussionmentioning

confidence: 99%

“…Cells grown at 20-100 lmol photons Á m À2 Á s À1 showed no peaks corresponding to a biliprotein (data not shown). A study on a species of Rhodomonas (now Pyrenomonas) by Chaloub et al (2015) showed similarly that the amount of phycoerythrin increased when cells were grown at low light levels (<15 lmol photons Á m À2 Á s À1 ) compared to 50 and 150 lmol photons Á m À2 Á s À1 . In addition, Chaloub et al (2015) showed that the phycoerythrin content decreased in Rhodomonas cells approaching the stationary phase.…”

Section: Niels Daugbjerg Et Almentioning

confidence: 95%

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Baffinella frigidus gen. et sp. nov. (Baffinellaceae fam. nov., Cryptophyceae) from Baffin Bay: Morphology, pigment profile, phylogeny, and growth rate response to three abiotic factors

Daugbjerg

Norlin

Lovejoy

2018

Journal of Phycology

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Twenty years ago an Arctic cryptophyte was isolated from Baffin Bay and given strain number CCMP2045. Here, it was described using morphology, water- and non-water soluble pigments and nuclear-encoded SSU rDNA. The influence of temperature, salinity, and light intensity on growth rates was also examined. Microscopy revealed typical cryptophyte features but the chloroplast color was either green or red depending on the light intensity provided. Phycoerythrin (Cr-PE 566) was only produced when cells were grown under low-light conditions (5 μmol photons · m · s ). Non-water-soluble pigments included chlorophyll a, c and five major carotenoids. Cells measured 8.2 × 5.1 μm and a tail-like appendage gave them a comma-shape. The nucleus was located posteriorly and a horseshoe-shaped chloroplast contained a single pyrenoid. Ejectosomes of two sizes and a nucleomorph anterior to the pyrenoid were discerned in TEM. SEM revealed a slightly elevated vestibular plate in the vestibulum. The inner periplast component consisted of slightly overlapping hexagonal plates arranged in 16-20 oblique rows. Antapical plates were smaller and their shape less profound. Temperature and salinity studies revealed CCMP2045 as stenothermal and euryhaline and growth was saturated between 5 and 20 μmol photons · m · s . The phylogeny based on SSU rDNA showed that CCMP2045 formed a distinct clade with CCMP2293 and Falcomonas sp. isolated from Spain. Combining pheno- and genotypic data, the Arctic cryptophyte could not be placed in an existing family and genus and therefore Baffinellaceae fam. nov. and Baffinella frigidus gen. et sp. nov. were proposed.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Niels Daugbjerg Et Almentioning

confidence: 95%

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Baffinella frigidus gen. et sp. nov. (Baffinellaceae fam. nov., Cryptophyceae) from Baffin Bay: Morphology, pigment profile, phylogeny, and growth rate response to three abiotic factors

Daugbjerg

Norlin

Lovejoy

2018

Journal of Phycology

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“…Microalgae were batch-cultured in two culture conditions with a working volume of 300 mL (Table 1). Before starting the experiments, R. salina was cultured in a 500-mL erlenmeyer, with 0.2-mM filtered seawater with a modified f/2 medium (Guillard, 1975in Andersen, Berges, Harrison, & Watanabe, 2005, at optimal growth condition according to Chaloub et al (2015). In both cultures (containing f/2 medium and inoculum), microalgae were grown in natural seawater except that the salinity of C1 culture was modified.…”

Section: Cultivation Of R Salina Microalgaementioning

confidence: 99%

“…According to several studies, PE production from Rhodomonas sp. is inf luenced by nitrate concentration, photon flux density (light intensity), and temperature (Bartual, Lubián, Gálvez, & Niell, 2002;Chaloub, Motta, de Araujo, de Aguiar, & da Silva,, 2015;da Silva, Lourenco, & Chaloub, 2009;Vu et al, 2015). Compared to chlorophyll, other pigments or accessories pigments, i.e.…”

Section: Introductionmentioning

confidence: 99%

Influence of Salinity on Growth and Phycoerythrin Production of Rhodomonas salina

Marraskuranto¹,

Raharjo²,

Kasiamdari³

et al. 2018

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Microalgae is a photoautotroph organism capable of producing various photosynthetic pigments with diverse beneficial properties. Rhodomonas salina, a Cryptophyte cell, contains only phycoerythrin as its phycobiliprotein pigment. The effects of salinity on growth and phycoerythrin concentration were investigated. Microalgae R. salina were grown in natural sea water with salinity of 33‰ and 50‰.The microalgae was batch-cultured in f/2 medium at light irradiation of 1100 lux, temperature of 24-26 o C, and photoperiode of 12 h : 12 h. The microalgae cell density was directly calculated using haemacytometer. The concentration of phycoerythrin was determined by spectrophotometric method. The cell density and phycoerythrin concentration were monitored every 4 days for 20 days of cell growth. Results showed that salinity did not affect significantly both on growth and phycoerythrin concentration extracted from R. salina biomass (p>0.05;  = 0.05). At both salinity, maximum phycoerythrin concentration were reached on day 8. There was a positive correlation between cell density and phycoerythrin concentration from day 1 to day 8 of cell growth. Microalgae R. salina which was grown in natural seawater with salinity of 33‰ achieved the highest cell density of 8.4 x 10 5 cells/mL and the phycoerythrin concentration of 0.19 g. 10 -5 cell on day 8 of the culture. The highest phycoerythrin concentration was obtained on day 16 of the culture i.e 0.27 g. 10 -5 cell.

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Algae in Biotechnological Processes

Niemczyk

Żyszka-Haberecht

Drzyzga

et al. 2018

Algae Biomass: Characteristics and Applications

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Combined effects of irradiance, temperature and nitrate concentration on phycoerythrin content in the microalga Rhodomonas sp. (Cryptophyceae)

Cited by 57 publications

References 33 publications

Baffinella frigidus gen. et sp. nov. (Baffinellaceae fam. nov., Cryptophyceae) from Baffin Bay: Morphology, pigment profile, phylogeny, and growth rate response to three abiotic factors

Baffinella frigidus gen. et sp. nov. (Baffinellaceae fam. nov., Cryptophyceae) from Baffin Bay: Morphology, pigment profile, phylogeny, and growth rate response to three abiotic factors

Influence of Salinity on Growth and Phycoerythrin Production of Rhodomonas salina

Algae in Biotechnological Processes

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