Influence of Salinity on Growth and Phycoerythrin Production of Rhodomonas salina

Marraskuranto, Endar; Raharjo, Tri Joko; Kasiamdari, Rina Sri; Nuringtyas, Tri Rini

doi:10.15578/squalen.v13i3.365

Cited by 7 publications

(6 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The monitoring of PE production showed that the highest PE yield was reached on day 16 of culture, which correspond to the stationary phase. This result is in concordance with previous data reported by Marraskuranto et al in 2018 [ 44 ]. Many publications support that abiotic factors have substantial effects on biomass and pigments production.…”

Section: Discussionsupporting

confidence: 94%

Improvement of Biomass and Phycoerythrin Production by a Strain of Rhodomonas sp. Isolated from the Tunisian Coast of Sidi Mansour

et al. 2022

View full text Add to dashboard Cite

Microalgae are photoautotrophic microorganisms known as producers of a large variety of metabolites. The taxonomic diversity of these microorganisms has been poorly explored. In this study, a newly isolated strain was identified based on the 18S rRNA encoding gene. The phylogenetic analysis showed that the isolated strain was affiliated with the Rhodomonas genus. This genus has greatly attracted scientific attention according to its capacity to produce a large variety of metabolites, including phycoerythrin. Growth and phycoerythrin production conditions were optimized using a Plackett–Burman design and response surface methodology. An expression profile analysis of the cpeB gene, encoding the beta subunit of phycoerythrin, was performed by qRT-PCR under standard and optimized culture conditions. The optimization process showed that maximum cell abundance was achieved under the following conditions: CaCl2 = 2.1328 g/L, metal solution = 1 mL/L, pH = 7 and light intensity = 145 μmol photons/m2/s, whereas maximum phycoerythrin production level occurred when CaCl2 = 1.8467 g/L, metal solution = 1 mL/L, pH = 7 and light intensity = 157 μmol/m2/s. In agreement, positive transcriptional regulation of the cpeB gene was demonstrated using qRT-PCR. This study showed the successful optimization of abiotic conditions for highest growth and phycoerythrin production, making Rhodomonas sp. suitable for several biotechnological applications.

show abstract

Section: Discussionsupporting

confidence: 94%

Improvement of Biomass and Phycoerythrin Production by a Strain of Rhodomonas sp. Isolated from the Tunisian Coast of Sidi Mansour

et al. 2022

View full text Add to dashboard Cite

show abstract

“…No effect of the increase in salinity was observed in our material. Similar patterns were observed in Compsopogon, also a blue rhodophyte (Glazer et al, 1994), along with other red algae or cryptophytes such as Rhodomonas salina (Marraskuranto et al, 2018). The promotion of PBP synthesis in increasing salinity was demonstrated in Spirulina platensis by Sharma et al (2014), but Lu & Vonshak (2002) observed that the PC content decreased in salt-stressed cells of the species.…”

Section: Effect Of the Increase In Nitrogen And Salinitysupporting

confidence: 64%

Confocal Microscopy in Ecophysiological Studies of Algae: A Door to Understanding Autofluorescence in Red Algae

2021

View full text Add to dashboard Cite

Alga in the genus Chroothece have been reported mostly from aquatic or subaerial continental environments, where they grow in extreme conditions. The strain Chroothece mobilis MAESE 20.29 was exposed to different light intensities, red and green monochromatic light, ultraviolet (UV) radiation, high nitrogen concentrations, and high salinity to assess the effect of those environmental parameters on its growth. Confocal laser scanning microscopy (CLSM) was used as an “in vivo” noninvasive single-cell method for the study. The strain seemed to prefer fairly high light intensities and showed a significant increase in allophycocyanin (APC) and chlorophyll a [photosystem I (PSI) and photosystem II (PSII)] fluorescence with 330 and 789 μM/cm2/s intensities. Green monochromatic light promoted a significant increase in the fluorescence of APC and chlorophyll a (PSI and PSII). UV-A significantly decreased phycocyanin and increased APC, while UV-A + B showed a greater decreasing effect on c-Phycocyanin but did not significantly change concentrations of APC. The increase in nitrogen concentration in the culture medium significantly and negatively affected all pigments, and no effect was observed with an increase in salinity. Our data show that CLSM represents a very powerful tool for ecological research of microalgae in small volumes and may contribute to the knowledge of phycobiliproteins in vivo behavior and the parameters for the large-scale production of these pigments.

show abstract

“…cultivation has been conducted in aspects of biomass production rate and biochemical composition, but no commercially stable cultivation plan has been applied in aquaculture yet (Seixas et al 2009;Oostlander et al 2020;Yamamoto et al 2020). There is literature focused on the absorption characteristics of PE in Rhodomonas (Van Der Weij-De Wit et al 2006;da Silva et al 2009;Marraskuranto et al 2018), but no literature investigated the growth of this cryptophytic species under different light qualities. This paper fills the gap and provides the knowledge necessary to design a sound cultivation plan for Rhodomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Effect of light quality and quantity on productivity and phycoerythrin concentration in the cryptophyte Rhodomonas sp.

et al. 2021

View full text Add to dashboard Cite

The cryptophyte Rhodomonas sp. is a potential feed source for aquaculture live feed and resource for phycoerythrin (PE) production. This research investigates the influence of light, both quality and quantity, on the biomass productivity, composition and growth rate of Rhodomonas sp. The incident light intensity used in the experiments was 50 μmolphotons m−2 s−1, irrespective of the colour of the light, and cultivation took place in lab-scale flat-panel photobioreactors in turbidostat mode. The highest productivity in volumetric biomass (0.20 gdry weight L−1 day−1), measured under continuous illumination, was observed under green light conditions. Blue and red light illumination resulted in lower productivities, 0.11 gdry weight L−1 day−1 and 0.02 g L−1 day−1 respectively. The differences in production are ascribed to increased absorption of green and blue wavelength by phycoerythrin, chlorophyll and carotenoids, causing higher photosynthetically usable radiation (PUR) from equal photosynthetically absorbed irradiance (PAR). Moreover, phycoerythrin concentration (281.16 mg gDW−1) was stimulated under red light illumination. Because photosystem II (PSII) absorbs poorly red light, the algae had to induce more pigments in order to negate the lower absorption per unit pigment of the incident available photons. The results of this study indicate that green light can be used in the initial growth of Rhodomonas sp. to produce more biomass and, at a later stage, red light could be implemented to stimulate the synthesis of PE. Fourier-transform infrared spectroscopy (FTIR) analysis demonstrated a significant difference between the cells under different light quality, with higher contents of proteins for samples of Rhodomonas sp. cultivated under green light conditions. In comparison, higher carbohydrate contents were observed for cells that were grown under red and blue light.

show abstract

Influence of Salinity on Growth and Phycoerythrin Production of Rhodomonas salina

Cited by 7 publications

References 14 publications

Improvement of Biomass and Phycoerythrin Production by a Strain of Rhodomonas sp. Isolated from the Tunisian Coast of Sidi Mansour

Improvement of Biomass and Phycoerythrin Production by a Strain of Rhodomonas sp. Isolated from the Tunisian Coast of Sidi Mansour

Confocal Microscopy in Ecophysiological Studies of Algae: A Door to Understanding Autofluorescence in Red Algae

Effect of light quality and quantity on productivity and phycoerythrin concentration in the cryptophyte Rhodomonas sp.

Contact Info

Product

Resources

About