Combined effect of mutations in ADSL and GARS-AIRS-GART genes on IMP content in chickens

Abstract

1. The objective of this study was to investigate the effect of ADSL gene, GARS-AIRS-GART gene and their combination genotype on inosine monophosphate content (IMP) in chicken. 2. The chicken breeds used for this study were Recessive White chicken (RW, Jiang-13 strain of white Plymouth Rock) and preserved population of 4 Chinese native chicken breeds, including Silkies, Baier, Tibetan and Xiaoshan. 3. The primers for exon 2 in ADSL gene and 5'UTR region in GARS-AIRS-GART gene were designed and the single nucleotide polymorphisms (SNPs) were detected by PCR-SSCP and DNA sequencing. 4. Two SNPs were detected, C/T substitution at position 3484 in exon 2 of ADSL gene, which was a silent mutation, and C/T point mutation at position -179 in 5'UTR region of GARS-AIRS-GART gene. In ADSL gene, individuals with TT genotype had significantly higher IMP content than CT and CC genotype individuals. No significant difference was observed between CT and CC genotypes. Similar results were obtained for GARS-AIRS-GART gene. The combination of genotypes ADSL and GARS-AIRS-GART genes also had a significant effect on IMP content. Individuals with TTTT genotype had the highest muscle IMP content, while individuals with CCCT genotype had the lowest. 4. We putatively drew the conclusion that the SNPs in these two genes, as well as the combination genotypes, could be used as potential molecular markers for meat quality in chicken.

Correlation between polymorphisms inADSLandGARS-AIRS-GARTgenes with inosine 5′-monophosphate (IMP) contents in Beijing-you chickens

“…In agreement with Shu et al (2007b), individuals inheriting EE (or MM) genotypes at the ADSL (or GARS-AIRS-GART) genes had significantly higher IMP content in breast muscle than did those inheriting FF (or NN) genotypes. In the present study, there was no significant difference in IMP between EE and EF, nor between MM and MN genotypes.…”

“…The products of the ADSL (adenylosuccinate lyase deficiency) gene and the GARS-AIRS-GART (glycinamide ribonucleotide synthetaseaminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase) gene are two key enzymes that are involved in the synthesis of IMP. Chicken ADSL and GARS-AIRS-GART genes have been studied as candidate genes for the effects on the IMP content in muscle (Shu et al, 2007b) and favourable association between genotypes and higher IMP content has been demonstrated in several Chinese native chicken breeds. Beijing-you (BJY) is a Chinese local chicken breed with a history of nearly 300 years.…”

Association between ADSL, GARS-AIRS-GART, DGAT1, and DECR1 expression levels and pork meat quality traits

“…Recent studies have reported that ADSL and GARS-AIRS-GART affect IMP content in chickens (Shu et al, 2009;Ye et al, 2010). In this study, ADSL and GARS-AIRS-GART had high expression levels in LL and low expression levels in heart and liver.…”

“…ADSL and GARS-AIRS-GART are enzymes involved in the biosynthesis of purine nucleotides, which contribute to delicate flavors in muscle tissue (Kan and Moran, 1995;Shu et al, 2009). Recent studies have reported that ADSL and GARS-AIRS-GART affect IMP content in chickens (Shu et al, 2009;Ye et al, 2010).…”

“…Single nucleotide polymorphisms in ADSL, a gene that codes for adenylosuccinate lyase, and in GARS-AIRS-GART, a gene that codes for glycinamide ribonucleotide-aminoimidazole ribonucleotide synthetaseglycinamide ribonucleotide transformylase, affect muscle inosine monophosphate (IMP) content in chickens (Shu et al, 2007(Shu et al, , 2009Ye et al, 2010). The gene DGAT1, which affects IMP content by increasing triglyceride levels in pig muscle (Nonneman and Rohrer, 2002), is associated with improved meat quality in Landrace swine.…”