Combined detection of C-reactive protein and PBMC quantification from whole blood in an integrated lab-on-a-disc microfluidic platform

Uddin, Rokon; Donolato, Marco; Hwu, En‐Te; Hansen, Mikkel Fougt; Boisen, Anja

doi:10.1016/j.snb.2018.07.015

Cited by 19 publications

(8 citation statements)

References 52 publications

(54 reference statements)

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“…These sensitivities are much lower than the ones obtained in the other systems addressed in this study, which points out the lower binding affinities of the probes used in this immunoassay. However, despite these low SNR values, this sensitivity is in the concentration range of CRP in healthy humans plasma (between 3 and 10 µg mL -1 ), which increases dramatically under cardiovascular diseases, inflammations, and infections (Kowalczyk et al, 2018;Uddin et al, 2018).…”

Section: Immunosensingmentioning

confidence: 98%

Disk-based one-dimensional photonic crystal slabs for label-free immunosensing

Sancho-Fornes

Avella-Oliver

Carrascosa

et al. 2019

Biosensors and Bioelectronics

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One-dimensional photonic crystal slabs are periodic optical nanostructures that produce guided-mode resonance. They couple part of the incident light into the waveguide generating bandgaps in the transmittance spectrum, whose position is sensitive to refractive index variations on their surface. In this study, we present one-dimensional photonic crystal slab biosensors based on the internal nanogrooved structure of Blu-ray disks for label-free immunosensing. We demonstrated that this polycarbonate structure coated with a critical thickness of TiO2 generates guided-mode resonance. Its optical behavior was established comparing it with other compact disk structures. The results were theoretically calculated and experimentally demonstrated, all them being in agreement. The bioanalytical performance of these photonic crystals was experimentally demonstrated in a model assay to quantify IgGs as well as in two immunoassays to determine the biomarkers C-reactive protein and lactate dehydrogenase (detection limits of 0.1, 87, and 13 nM, respectively). The results are promising towards the development of new low-cost, portable, and label-free optical biosensors that join these photonic crystals with dedicated bioanalytical scanners based on compact disk drives.

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Section: Immunosensingmentioning

confidence: 98%

Disk-based one-dimensional photonic crystal slabs for label-free immunosensing

Sancho-Fornes

Avella-Oliver

Carrascosa

et al. 2019

Biosensors and Bioelectronics

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“…Prominent examples of integrated microfluidic platforms where flow control elements coordinate the spatio-temporal arrangement of a range of LUOs towards sample-to-answer automation are based on rotationally induced centrifugal fields [17][18][19][20][21][22][23][24][25][26], electrokinetics [27], acoustophoresis [28][29][30][31], and digital (droplet) microfluidics on electrowetting-on-dielectric (EWOD) [32][33][34][35][36], surface acoustic waves (SAWs) [37][38][39] and multiphase flows [40][41][42], possibly supported by externally actuated precision pumps and mechanical valves. In this work, we focus on microfluidic technologies for automating typical in vitro procedures in life-science laboratories.…”

Section: Integrated Microfluidic Platformsmentioning

confidence: 99%

Efficient Development of Integrated Lab-On-A-Chip Systems Featuring Operational Robustness and Manufacturability

Ducrée

2019

Micromachines

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The majority of commercially oriented microfluidic technologies provide novel point-of-use solutions for laboratory automation with important areas in the context of the life sciences such as health care, biopharma, veterinary medicine and agrifood as well as for monitoring of the environment, infrastructures and industrial processes. Such systems are often composed of a modular setup exhibiting an instrument accommodating rather conventional actuation, detection and control units which interfaces with a fluidically integrated “Lab-on-a-Chip” device handling (bio-)sample(s) and reagents. As the complex network of tiny channels, chambers and surface-functionalised zones can typically not be properly cleaned and regenerated, these microfluidic chips are mostly devised as single-use disposables. The availability of cost-efficient materials and associated structuring, functionalisation and assembly schemes thus represents a key ingredient along the commercialisation pipeline and will be a first focus of this work. Furthermore, and owing to their innate variability, investigations on biosamples mostly require the acquisition of statistically relevant datasets. Consequently, intermediate numbers of consistently performing chips are already needed during application development; to mitigate the potential pitfalls of technology migration and to facilitate regulatory compliance of the end products, manufacture of such pilot series should widely follow larger-scale production schemes. To expedite and de-risk the development of commercially relevant microfluidic systems towards high Technology Readiness Levels (TRLs), we illustrate a streamlined, manufacturing-centric platform approach employing the paradigms of tolerance-forgiving Design-for-Manufacture (DfM) and Readiness for Scale-up (RfS) from prototyping to intermediate pilot series and eventual mass fabrication. Learning from mature industries, we further propose pursuing a platform approach incorporating aspects of standardisation in terms of specification, design rules and testing methods for materials, components, interfaces, and operational procedures; this coherent strategy will foster the emergence of dedicated commercial supply chains and also improve the economic viability of Lab-on-a-Chip systems often targeting smaller niche markets by synergistically bundling technology development.

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“…Pre-analytic protocols such as blood–plasma separation [ 15 ] and pre-treatment of whole saliva [ 16 ] can be easily implemented. These are some reasons why many different centrifugal system-based immunoassay solutions have been shown, using different assay methods and with different applications [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

ImmunoDisk—A Fully Automated Bead-Based Immunoassay Cartridge with All Reagents Pre-Stored

et al. 2022

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In this paper, we present the ImmunoDisk, a fully automated sample-to-answer centrifugal microfluidic cartridge, integrating a heterogeneous, wash-free, magnetic- and fluorescent bead-based immunoassay (bound-free phase detection immunoassay/BFPD-IA). The BFPD-IA allows the implementation of a simple fluidic structure, where the assay incubation, bead separation and detection are performed in the same chamber. The system was characterized using a C-reactive protein (CRP) competitive immunoassay. A parametric investigation on air drying of protein-coupled beads for pre-storage at room temperature is presented. The key parameters were buffer composition, drying temperature and duration. A protocol for drying two different types of protein-coupled beads with the same temperature and duration using different drying buffers is presented. The sample-to-answer workflow was demonstrated measuring CRP in 5 µL of human serum, without prior dilution, utilizing only one incubation step, in 20 min turnaround time, in the clinically relevant concentration range of 15–115 mg/L. A reproducibility assessment over three disk batches revealed an average signal coefficient of variation (CV) of 5.8 ± 1.3%. A CRP certified reference material was used for method verification with a concentration CV of 8.6%. Our results encourage future testing of the CRP-ImmunoDisk in clinical studies and its point-of-care implementation in many diagnostic applications.

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Combined detection of C-reactive protein and PBMC quantification from whole blood in an integrated lab-on-a-disc microfluidic platform

Cited by 19 publications

References 52 publications

Disk-based one-dimensional photonic crystal slabs for label-free immunosensing

Disk-based one-dimensional photonic crystal slabs for label-free immunosensing

Efficient Development of Integrated Lab-On-A-Chip Systems Featuring Operational Robustness and Manufacturability

ImmunoDisk—A Fully Automated Bead-Based Immunoassay Cartridge with All Reagents Pre-Stored

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