Combinatorial expression of cell cycle regulators is more suitable for immortalization than oncogenic methods in dermal papilla cells

Fukuda, T.; Furuya, Kai; Takahashi, Kazumasa; Orimoto, Ai; Sugano, Eriko; Tomita, Hiroshi; Kashiwagi, Sayo; Kiyono, Tohru; Ishii, Tsuyoshi

doi:10.1016/j.isci.2020.101929

Cited by 13 publications

(10 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1B. The number of reads per sample ranged from 16 M to 23 M, which is consistent with our previous analysis 14 . The mapping ratio to the reference genome was approximately 95 to 96.3%, which is sufficient for quantifying gene expression 5,15 .…”

Section: Data Recordssupporting

confidence: 90%

Comprehensive transcriptome data to identify downstream genes of testosterone signalling in dermal papilla cells

Matsusaka

Furuya

et al. 2022

Sci Data

Self Cite

View full text Add to dashboard Cite

Testosterone-related steroid hormones are associated with various types of diseases, including prostate cancer and androgenetic alopecia (AGA). The testosterone or dihydroxy testosterone (DHT) circulates through the blood, binds to the androgen receptor (AR) in the cytoplasm, and finally enters the nucleus to activate downstream target genes. We previously found that immortalized dermal papilla cells (DPCs) lost AR expression, which may be explained by the repeated cell passages of DPCs. To compensate for the AR expression, DPCs that express AR exogenously were established. In this study, we performed an RNA-Seq analysis of the AR-expressing and non-AR-expressing DPCs in the presence or absence of DHT to identify the downstream target genes regulated by AR signalling. Furthermore, we treated DPCs with minoxidil sulphate, which has the potential to treat AGA. This is the first comprehensive analysis to identify the downstream genes involved in testosterone signalling in DPCs. Our manuscript provides high-priority data for the discovery of molecular targets for prostate cancer and AGA.

show abstract

Section: Data Recordssupporting

confidence: 90%

Comprehensive transcriptome data to identify downstream genes of testosterone signalling in dermal papilla cells

Matsusaka

Furuya

et al. 2022

Sci Data

Self Cite

View full text Add to dashboard Cite

show abstract

“…In our previous study of human dermal papilla cell, SV40 immortalization caused more larger effect to the whole transcriptome than that of K4DT. [ 16 ] We cannot explain exact reason for the inconsistent results between mink whale and human dermal papilla cell. However, there might be species specific response even against the virus derived oncogene product.…”

Section: Discussionmentioning

confidence: 99%

“…However, due to the loss of function of the p53 protein, which is essential to maintaining the intact chromosome condition, chromosomal abnormalities are frequently observed as genomic instability. [ 15,16 ] The critical role of the p53 tumor suppressor can be characterized as gradian of the genome. [ 17 ] As an alternative method of immortalization, combinational expression of R24C mutant type cyclin‐dependent kinase 4 (CDK4) and cyclin D1, and telomerase reverse transcriptase (TERT) has been reported in human myoblasts.…”

Section: Introductionmentioning

confidence: 99%

“…[ 18 ] It should be noted that the combined expression of mutant CDK4, cyclin D1, and TERT enabled infinite cell proliferation without genomic instability, which may explain the intact function of the p53 tumor suppressor. [ 16,19 ] Of these genes, CDK4, cyclin D1, and TERT, was named as “K4DT” method. [ 18 ] Furthermore, we demonstrated that the K4DT method could be applied to immortalize cells derived from multiple species, including human, [ 20 ] bovine, [ 21 ] swine, [ 22 ] monkey, [ 23 ] midget buffalo, [ 24 ] wild cat, [ 25 ] prairie vole, [ 26 ] rat, [ 27 ] and sea turtle.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Common Minke Whale (Balaenoptera Acutorostrata) Cell Lines Immortalized with the Expression of Cell Cycle Regulators

Sekine,

Yasunaga,

Kumamoto

et al. 2023

Advanced Biology

Self Cite

View full text Add to dashboard Cite

Primary cultured cells cannot proliferate infinite. The overcoming of this limit can be classified as immortalization. Bypass of p16 senescence protein induces efficient immortalization various types of mammalians is previously reported. However, the Cetacea species is not known. Here, that common minke whale‐derived cells can be immortalized with a combination of human genes, mutant cyclin‐dependent kinase 4 (CDK4R24C), cyclin D1, and Telomerase Reverse Transcriptase (TERT) is reported. These results indicate that the function of cell cycle regulators in premature senescence is evolutionarily conserved. This study describes the conserved roles of cell cycle regulators in the immortalization of cells from humans to Cetacea species. Furthermore, using RNA‐seq based on next‐generation sequencing, the gene expression profiles of immortalized cells are compared with parental cells as well as those immortalized with SV40 large T antigen, which is once a popular method for cellular immortalization. The profiling results show that newly established common minke‐whale‐derived immortaliozed cells have completely different profiles from SV40 cells. This result indicates that the expression of mutant CDK4, cyclin D1, and TERT enables to establish immortalized cell lines with different biological nature from SV40 expressing cells.

show abstract

“…Furthermore, there are many reports of cell lines derived from diseased tissues [ 9 ] and transformed cells by overexpression of hTERT [ 10 ]. In addition, cells have been efficiently immortalized in combination with cell cycle regulators [ 11 , 12 ]. Immortalization also alters cell properties, with changes in gene expression profiles and karyotypes [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Novel cell lines derived from Chinese hamster kidney tissue

Kawabe

Kamihira

2022

PLoS ONE

View full text Add to dashboard Cite

Immortalized kidney cell lines are widely used in basic and applied research such as cell permeability tests and drug screening. Although many cell lines have been established from kidney tissues, the immortalization process has not been clarified in these cell lines. In this study, we analyzed the phenotypic changes that occurred during the immortalization of kidney cells derived from Chinese hamster tissue in terms of karyotype and gene expression profiles. In the newly established cell line, designated as CHK-Q, gene expression profiles at each stage of the immortalization process and during the adaptation to serum-free conditions were analyzed by DNA microarray. Renal stem cell markers CD24 and CD133 were expressed in CHK-Q cells, suggesting that CHK-Q cells were transformed from renal stem cells. Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis to identify the pathways of upregulated and downregulated genes revealed that the immortalization of CHK-Q cells was associated with increased fluctuations in the expression of specific proto-oncogenes. Karyotype analysis of spontaneously immortalized CHK-Q cells indicated that CHK-Q chromosomes had a typical modal number of 23 but possessed slight chromosomal abnormalities. In this study, we investigated the mechanism of cell environmental adaptation by analyzing gene expression behavior during the immortalization process and serum-free adaptation. CHK-Q cells are applicable to the fields of biotechnology and biomedical science by utilizing their characteristics as kidney-derived cells.

show abstract

Combinatorial expression of cell cycle regulators is more suitable for immortalization than oncogenic methods in dermal papilla cells

Cited by 13 publications

References 23 publications

Comprehensive transcriptome data to identify downstream genes of testosterone signalling in dermal papilla cells

Comprehensive transcriptome data to identify downstream genes of testosterone signalling in dermal papilla cells

Characterization of Common Minke Whale (Balaenoptera Acutorostrata) Cell Lines Immortalized with the Expression of Cell Cycle Regulators

Novel cell lines derived from Chinese hamster kidney tissue

Contact Info

Product

Resources

About