Comprehensive transcriptome data to identify downstream genes of testosterone signalling in dermal papilla cells

Abstract

Testosterone-related steroid hormones are associated with various types of diseases, including prostate cancer and androgenetic alopecia (AGA). The testosterone or dihydroxy testosterone (DHT) circulates through the blood, binds to the androgen receptor (AR) in the cytoplasm, and finally enters the nucleus to activate downstream target genes. We previously found that immortalized dermal papilla cells (DPCs) lost AR expression, which may be explained by the repeated cell passages of DPCs. To compensate for the AR expression, DPCs that express AR exogenously were established. In this study, we performed an RNA-Seq analysis of the AR-expressing and non-AR-expressing DPCs in the presence or absence of DHT to identify the downstream target genes regulated by AR signalling. Furthermore, we treated DPCs with minoxidil sulphate, which has the potential to treat AGA. This is the first comprehensive analysis to identify the downstream genes involved in testosterone signalling in DPCs. Our manuscript provides high-priority data for the discovery of molecular targets for prostate cancer and AGA.

Characterization of Common Minke Whale (Balaenoptera Acutorostrata) Cell Lines Immortalized with the Expression of Cell Cycle Regulators

“…The mapping ratio was ≈87-93%, which is similar to our previous data on humanderived cells. [7,30] To estimate the cell origin of parental primary cell, we carried out the comparison of whole transcriptome with RNA-Seq data of of brain(SRR918699), heart(SRR918701), lung(SRR922125), kidney(SRR919295), liver(SRR919296), muscle(SRR922171, SRR922446) of Balaenoptera acutorostrata, and fibroblasts of Balaenoptera musculus(SRR11045090). As the result of 3D principle component analysis (PCA), the expression profiling of parental cell has mapped into the most close position from fibroblasts of Balaenoptera musculus (Figure 5D).…”