Combinatorial effect of substratum properties on mesenchymal stem cell sheet engineering and subsequent multi-lineage differentiation

Chuah, Yon Jin; Zhang, Ying; Wu, Yingnan; Menon, Nishanth Venugopal; Goh, Ghim Hian; Lee, Ann Charlene; Chan, Vincent; Zhang, Yilei; Kang, Yuejun

doi:10.1016/j.actbio.2015.05.023

Cited by 48 publications

(37 citation statements)

References 66 publications

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“…The reciprocity between MSCs and their micro‐environmental cues in defects is important for the specific differentiation fates of MSCs (Figure ; Jiang & Papoutsakis, ; Moore & Lemischka, ). Plentiful research has shown that the mechanical properties of the micro‐environment can affect the cell behaviour of MSCs, including adhesion, proliferation, migration, and differentiation (Chuah et al, ; Keogh et al, ; Swift et al, ). MSCs can store the surface stiffness information from niches and influence their fate (Lee, Abdeen, & Kilian, ).…”

Section: Discussionmentioning

confidence: 99%

“…Plentiful research has shown that the mechanical properties of the micro-environment can affect the cell behaviour of FIGURE 9 The scheme illustrating the effects of mineralized collagen membranes with different surface micromechanical properties on regeneration of bone defects. MSC = mesenchymal stem cell [Colour figure can be viewed at wileyonlinelibrary.com] MSCs, including adhesion, proliferation, migration, and differentiation (Chuah et al, 2015;Keogh et al, 2010;Swift et al, 2013). MSCs can store the surface stiffness information from niches and influence their fate (Lee, Abdeen, & Kilian, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that the proliferation and differentiation behaviours of stem cells are affected by their micro-environment in situ, which is termed their stem-cell niche (Chuah et al, 2015;Jiang & Papoutsakis, 2013). The stem-cell niche controls the fate of stem cells in the defect according to the properties of the supporting biomaterials (e.g., mechanical properties, surface biochemistry, and topography) and biochemical cues (e.g., Ca 2+ concentration, growth factors, and cytokines) in the micro-environment (Chuah et al, 2015;Swift et al, 2013). Several studies have shown that the individual matrix mechanics in the micro-environment can influence cellular behaviour, including cell attachment, proliferation, migration, and differentiation (Chen, Cao, et al, 2015;Chen, Dong, et al, 2015;Sun, Zhu, Hu, & Krebsbach, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Using biomimetically mineralized collagen membranes with different surface stiffness to guide regeneration of bone defects

Wang

Ye²,

Zhang

et al. 2018

J Tissue Eng Regen Med

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Because guided bone regeneration (GBR) process is pronouncedly affected by the micro-environment in the defect, the surface stiffness of collagen membranes as a constituent part of the micro-environment was investigated in this study. The objective of this study was to manufacture biomimetically mineralized collagen membranes with controllable surface stiffness based on biomimetic strategy and to investigate the influences of surface stiffness on GBR process. The characterization and biocompatibility of membranes were examined in vitro. The mechanical properties of membranes were evaluated on macro and micro levels using tensile test and atomic force microscope, respectively. The critical-size cranial defect model and ectopic osteogenesis were chosen to employ their performances in vivo. The results indicated that the biomimetically mineralized collagen membranes with controllable surface stiffness were manufactured based on the biomimetic theory. The in vitro experiments showed that the mineralized collagen membrane with satisfactory surface stiffness can better promote the adhesion, proliferation, and osteogenic differentiation of mesenchymal stem cells. The membranes can perform excellently in both osteoinduction and osteoconduction, which results in effective manifestations in aspects of ectopic osteogenesis and GBR in vivo. Therefore, this biomimetically mineralized collagen membrane is a promising candidate for GBR treatment in future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Using biomimetically mineralized collagen membranes with different surface stiffness to guide regeneration of bone defects

Wang

Ye²,

Zhang

et al. 2018

J Tissue Eng Regen Med

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“…MSCs can differentiate into various types of cell including osteoblasts, chondrocytes, adipocytes, neurons, and cardiomyocytes (Toma et al, 2002;Chuah et al, 2015;Pipino and Pandolfi, 2015;Zhuang et al, 2015) and exhibit beneficial effects such as angiogenesis and immune regulation (Castro-Manrreza and Montesinos, 2015;Kim and Cho, 2015). Potential functions of MSCs vary depending on the source and different experimental conditions, and MSCs can be used in clinical applications to treat different kinds of disease (Garcia-Olmo et al, 2009;Cho et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of angiogenic effects by hypoxia‐preconditioned human umbilical cord‐derived mesenchymal stem cells in a mouse model of hindlimb ischemia

Han

Kim

et al. 2015

Cell Biology International

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It has been studied that mesenchymal stem cells (MSCs) have the capability to promote angiogenesis. Furthermore, there is strong evidence that hypoxic conditions can enhance angiogenesis and immune modulation mediated by MSCs, a notion that has been applied in many fields of clinical application. In the present study, we compared the efficacy of hypoxia preconditioned human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and normoxia conditioned hUC-MSCs for the treatment of ischemic injury in hindlimbs of an immunodeficient mouse model. Expression of negative markers for MSC such as CD31, CD34, and CD45 or positive markers such as CD44, CD73, CD90, and CD105 was not significantly changed in hypoxia preconditioned hUC-MSCs compared with hUC-MSCs cultured in normoxic condition. Expression of angiogenesis-related genes such as COX-2, VEGF, Tie-2, and TGF-β1 was increased compared with hUC-MSCs cultured in normoxic conditions. In the in vivo model, CD31 expression as a marker of angiogenesis was significantly increased in the ischemic limbs at 1 month after injection with hypoxic hUC-MSCs. Angiogenesis-related genes such as Ang-1, COX-1, PIGF, and MCP-1 were significantly upregulated in the muscle of ischemic hindlimbs treated with hypoxic hUC-MSCs than normoxic hUC-MSCs. Expression of proinflammatory genes such as IL-1, and IL-20 was reduced, whereas TGF-β1, which has an anti-inflammatory effect, was strongly increased. In conclusion, hypoxic culture conditions could induce expression of angiogenesis related genes in hUC-MSCs, and hypoxia preconditioned hUC-MSCs showed enhancing effects by inducing angiogenesis and low inflammatory immune response compared with normoxic hUC-MSCs in the ischemia injured hindlimb of immunodeficient mice.

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“…Similar phenomena have been reported previously by Yuejun and his colleagues. 42 They found that hMSCs had higher metabolic activity on stiffer PDMS than the soft one while a higher differentiation activity of hMSCs were observed on soft PDMS compared to stiffer one. In addition, no significantly different metabolic activity was observed between CO and MW annealed scaffolds.…”

Section: Resultsmentioning

confidence: 98%

Flexible Yttrium-Stabilized Zirconia Nanofibers Offer Bioactive Cues for Osteogenic Differentiation of Human Mesenchymal Stromal Cells

Gazquez

Chen²,

Veldhuis

et al. 2016

ACS Nano

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Currently, the main drawback of ceramic scaffolds used in hard tissue regeneration is their low mechanical strength. Stabilized zirconia, especially the tetragonal 3% yttrium-stabilized zirconia (YSZ) phase, has been considered as a bioinert ceramic material with high mechanical strength. In the present work, flexible nanofibrous YSZ scaffolds were prepared by electrospinning. The obtained scaffolds showed remarkable flexibility at the macroscopic scale, while retaining their stiffness at the microscopic scale. The surface nanoroughness of the scaffolds could be tailored by varying the heat treatment method. Our results demonstrate that the osteogenic differentiation and mineralization of seeded human mesenchymal stromal cells were supported by the nanofibrous YSZ scaffolds, in contrast to the well-known bioinert behavior of bulk YSZ. These findings highlight that flexible ceramic scaffolds are an appealing alternative to the current brittle ceramics for bone tissue regeneration applications.

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Combinatorial effect of substratum properties on mesenchymal stem cell sheet engineering and subsequent multi-lineage differentiation

Cited by 48 publications

References 66 publications

Using biomimetically mineralized collagen membranes with different surface stiffness to guide regeneration of bone defects

Using biomimetically mineralized collagen membranes with different surface stiffness to guide regeneration of bone defects

Enhancement of angiogenic effects by hypoxia‐preconditioned human umbilical cord‐derived mesenchymal stem cells in a mouse model of hindlimb ischemia

Flexible Yttrium-Stabilized Zirconia Nanofibers Offer Bioactive Cues for Osteogenic Differentiation of Human Mesenchymal Stromal Cells

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