2018
DOI: 10.1038/s41598-017-18896-5
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Combination of high-fat/high-fructose diet and low-dose streptozotocin to model long-term type-2 diabetes complications

Abstract: The epidemic of type 2 diabetes mellitus (T2DM) is fueled by added fructose consumption. Here, we thus combined high-fat/high-fructose diet, with multiple low-dose injections of streptozotocin (HF/HF/Stz) to emulate the long-term complications of T2DM. HF/HF/Stz rats, monitored over 56 weeks, exhibited metabolic dysfunctions associated with the different stages of the T2DM disease progression in humans: an early prediabetic phase characterized by an hyperinsulinemic period with modest dysglycemia, followed by … Show more

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Cited by 127 publications
(106 citation statements)
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References 60 publications
(67 reference statements)
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“…The combination of high fat diet/high sugar with STZ has been extensively used for establishing T2DM animal models. This model has been reported to represent the pathogenesis of T2DM in humans . Typical symptoms experienced in T2DM animal models including increase in FGB, polyphagia, polydipsia and body weight reduction were evidently observed in all the diabetic rat groups.…”
Section: Discussionmentioning
confidence: 86%
See 1 more Smart Citation
“…The combination of high fat diet/high sugar with STZ has been extensively used for establishing T2DM animal models. This model has been reported to represent the pathogenesis of T2DM in humans . Typical symptoms experienced in T2DM animal models including increase in FGB, polyphagia, polydipsia and body weight reduction were evidently observed in all the diabetic rat groups.…”
Section: Discussionmentioning
confidence: 86%
“…The animals were fed with the HFD/HF for four weeks. After four weeks of dietary manipulation with HFD/HF solution, rats were fasted overnight and injected with freshly prepared solution of STZ (35 mg/kg) dissolved in 0.1 m of citrate buffer (pH 4.5) via intraperitoneal route . Three days after the administration of STZ, blood was obtained from the tail veins of the rats in other to measure the fasting blood glucose levels (FBG) with the aid of a glucose meter (Accu Check Performa, Roche Diagnostics, Germany).…”
Section: Methodsmentioning
confidence: 99%
“…High dosage of STZ has been reported to result in complete destruction of the beta cells, a model of type I diabetes. Recent studies report the effective use of low doses of STZ to induce insulin resistance, a model of type II diabetes (T2D) [25][26][27]. Administration of 10% fructose for two weeks followed by 40 mg/kg BW of STZ was demonstrated by Wilson and Islam to cause partial destruction of the beta cells and insulin resistance in rats, which are typical of T2D [28].…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, the current work was carried out to examine the beneficial properties of AITC on insulin resistance, oxidative stress, and transcription factors in T2DM induced by high‐fat diet (HFD)/streptozotocin (STZ) in rats. We used the HFD/STZ model as it is more efficient in establishing a T2DM rat model similar to human nonhemotoxic diabetes …”
Section: Introductionmentioning
confidence: 99%