Shorea roxburghii Leaf Extract Ameliorates Hyperglycemia Induced Abnormalities in High Fat/Fructose and Streptozotocin Induced Diabetic Rats

Abstract

This study investigated the hypoglycemic effect of the methanol extract of Shorea roxburghii leaves (SRL) in high fat diet/high fructose solution (HFDHF) and streptozotocin (STZ) induced type 2 diabetes mellitus (T2DM) in rats as well as evaluating its ameliorative potentials in altered biochemical and hematological parameters in the treated rats. T2DM was induced in Sprague Dawley (SD) rats by feeding with HFDHF for 4 weeks and administering STZ (35 mg/kg, i. p.). Diabetic rats were given SRL extract at doses of 100 and 400 mg/kg for 30 days. The food and water intake were monitored on a daily basis, while the fasting blood glucose (FBG) levels and body weight were measured weekly. Biochemical and hematological parameters as well as histopathological studies of the pancreas were also evaluated. SRL significantly decreased FBG and improved the body weight, food and water intake of treated diabetic rats. Furthermore, biochemical and hematological parameters including liver and kidney function enzymes, lipid profiles, white blood and red blood cells parameters were markedly ameliorated by SRL. Histopathological analyses of the pancreas indicated reconstitution of β‐cells architecture in SRL treated rats. The results of this study suggest that SRL has antidiabetic potential and can be considered for the treatment of T2DM.

Protective effects of ethyl acetate extract from Shorea roxburghii against diabetes induced testicular damage in rats

“…The selection of the doses of SRE used in this study was based on previous study 20 . The treatment started immediately after the confirmation of diabetes by oral gavage and lasted for 4 weeks.…”

“…According to previous reports, polyphenolic compounds, particularly stilbenoids and dihydroisocoumarins are the principal bioactive constituents present in S roxburghii 23 . Our previous study indicated that the stilbenoids rich extract (Figure 1) from the leaves of S roxburghii attenuated hyperglycemia induced biochemical and hematological alterations in diabetic rats 20 . However, there are no reports on the ameliorative effect of S roxburghii on diabetes induced testicular damage.…”

“…Shorea roxburghii is a medicinal plant used traditionally as an astringent, for treating cholera, dysentery and diarrhea 19 . The plant extract or its bioactive constituents confer several therapeutic benefits including antioxidant, hepatoprotective, cytotoxic, antihyperlipidemic and antidiabetic effects 20‐22 . According to previous reports, polyphenolic compounds, particularly stilbenoids and dihydroisocoumarins are the principal bioactive constituents present in S roxburghii 23 .…”

Antinociceptive, Anti‐Hyperalgesia and Antiallodynic Activities of Polyphenol Rich Extract fromShorea roxburghiiagainst Cyclophosphamide Induced Peripheral Neuropathy

“…Existing literatures have suggested that natural products especially medicinal plants and their constituent compounds have the ability to protect against multiple organ toxicities induced by CYP [13,14] . Shorea roxburghii is a Dipterocarpaceae tree that is rich in polyphenolic compounds, especially stilbenoids and dihydroisocoumarins [15] . Previous studies have shown that the extract from the leaves and stem back showed ameliorative and protective effects against diabetes induced testicular damage and d‐galactosamine/lipopolysaccharide‐induced liver injury, respectively [16,17] .…”

Therapeutic Effects of 5,7-Dihydroxy-6-Oxoheptadecanoic Acid on Dysglycemia, Dyslipidemia, and Other Complications in Diabetic Rats

“…33 Thereafter, the rats in the experimental group were administered STZ (35 mg/kg, dissolved in freshly prepared citrate buffer; pH 4.5) via intraperitoneal injection to induce diabetes. 34,35 The rats were confirmed diabetic after 3 days of injection by measuring the FBG in overnight fasted rats. Rats with FBG above 250 mg/dL were taken as diabetic and further divided into 2 groups with six rats in each group: the DCG and diabetic rat treated with DHA at a dose of 25 mg/kg (D + DHA).…”