Column coupling isotachophoresis–capillary electrophoresis with mass spectrometric detection: Characterization and optimization of microfluidic interfaces

Kler, Pablo A.; Posch, Tjorben Nils; Pattky, Martin; Tiggelaar, Roald M.; Hühn, Carolin

doi:10.1016/j.chroma.2013.04.046

Cited by 28 publications

(38 citation statements)

References 37 publications

(45 reference statements)

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“…Huhn et al. have developed a glass microfluidic chip for implementation of a hybrid modular system for performing 2D‐CE separations involving ITP with contactless conductivity detection as the first dimension separation and CZE with MS detection as the second dimension separation . Functionality of the system was demonstrated by performing preconcentration, separation, detection, and identification of four human angiotensin peptides.…”

Section: Separations In Different Ce and Cec Modesmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2013–middle 2015)

Kašička

2015

Electrophoresis

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The review brings a comprehensive survey of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, micropreparation, purification, and physicochemical and biochemical characterization of peptides in the years 2013, 2014, and ca. up to the middle of 2015. Advances in the investigation of electromigration properties of peptides, in the methodology of their analysis, including sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, as well as in detection of peptides, are described. New developments in particular CE and CEC modes are presented and several types of their applications to peptide analysis are reported: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence, and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Separations In Different Ce and Cec Modesmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2013–middle 2015)

Kašička

2015

Electrophoresis

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“…Kler et al. proposed a 2D electrophoresis approach coupling ITP to CE‐MS, which used a C 4 D to monitor the ITP step . Foret and co‐workers have used C 4 D as an auxiliary diagnostic tool in developing nanospray interfaces for CE‐MS .…”

Section: Introductionmentioning

confidence: 99%

A capillary electrophoresis system with dual capacitively coupled contactless conductivity detection and electrospray ionization tandem mass spectrometry

Francisco

Lago

2016

Electrophoresis

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A commercial system that is comprised of a CE coupled to an ESI triple quadrupole mass spectrometer was equipped with two capacitively coupled contactless conductivity detectors (C(4) Ds). The first C(4) D was positioned inside the original cartridge, and the second C(4) D was positioned as close as possible to the ESI probe entrance by using a 3D-printed support. The C(4) Ds electropherograms were matched to the ESI-MS electropherogram by correcting their timescales by the factor LT /LD , where LT and LD are the total capillary length and the length until the C(4) D, respectively. A general approach for method development supporting the simultaneous conductivity and MS detection is discussed, while application examples are introduced. These examples include the use of C(4) D as a simple device that dismiss the use of an EOF marker, a low-selectivity detector that continuously provide information about unexpected features of the sample, and even a detector that can be more sensitive than ESI-MS. The C(4) D used in this setup proved to have a smaller contribution to the peak broadening than ESI-MS, which allowed that a C(4) D, positioned at 12 cm from the inlet of an 80-cm-long capillary, could be used to foresee position and shape of the peaks being formed 6.8 times slower at the ESI-MS electropherogram.

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“…Over the last three decades, cationic ITP has been used in the preconcentration, separation, detection, and identification of different kinds of cationic proteins and their building blocks such as metal‐binding proteins , model peptides (i.e., human angiotensins) , and basic amino acids (arginine, histidine, and lysine) in pharmaceutical preparations . In the seminal work by Janssen et al , application of both cationic ITP and HPLC was performed to control the peptide purity of insulin, adrenocorticotropic hormone, and β‐endorphin.…”

Section: Introductionmentioning

confidence: 99%

“…In the seminal work by Janssen et al , application of both cationic ITP and HPLC was performed to control the peptide purity of insulin, adrenocorticotropic hormone, and β‐endorphin. In more recent work involving cationic ITP separations of proteins, the chosen electrolyte systems were comprised primarily of acetate‐based chemistry for both the LE and TE in the pH range of ∼4‐4.5 . These ITP electrolyte systems were able to successfully isotachophoretically separate basic amino acids (arginine, histidine, and lysine) and hormones (Lecirelin) in complex buffer solutions, and could be also be coupled with other detection schemes such as MS to lower detection limits .…”

Section: Introductionmentioning

confidence: 99%

“…In more recent work involving cationic ITP separations of proteins, the chosen electrolyte systems were comprised primarily of acetate‐based chemistry for both the LE and TE in the pH range of ∼4‐4.5 . These ITP electrolyte systems were able to successfully isotachophoretically separate basic amino acids (arginine, histidine, and lysine) and hormones (Lecirelin) in complex buffer solutions, and could be also be coupled with other detection schemes such as MS to lower detection limits . However, even with all the advances and applications involving cationic ITP separations of proteins, to the best of our knowledge, cationic ITP has never been run at pH 8 to demonstrate the effective separation of a low‐abundant serum protein (cTnI) from a high‐abundant serum protein (albumin).…”

Section: Introductionmentioning

confidence: 99%

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Cationic isotachophoresis separation of the biomarker cardiac troponin I from a high‐abundance contaminant, serum albumin

et al. 2014

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Cationic ITP was used to separate and concentrate fluorescently tagged cardiac troponin I (cTnI) from two proteins with similar isoelectric properties in a PMMA straight-channel microfluidic chip. In an initial set of experiments, cTnI was effectively separated from R-Phycoerythrin using cationic ITP in a pH 8 buffer system. Then, a second set of experiments was conducted in which cTnI was separated from a serum contaminant, albumin. Each experiment took ~ 10 min or less at low electric field strengths (34 V/cm) and demonstrated that cationic ITP could be used as an on-chip removal technique to isolate cTnI from albumin. In addition to the experimental work, a 1D numerical simulation of our cationic ITP experiments has been included to qualitatively validate experimental observations.

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Column coupling isotachophoresis–capillary electrophoresis with mass spectrometric detection: Characterization and optimization of microfluidic interfaces

Cited by 28 publications

References 37 publications

Recent developments in capillary and microchip electroseparations of peptides (2013–middle 2015)

Recent developments in capillary and microchip electroseparations of peptides (2013–middle 2015)

A capillary electrophoresis system with dual capacitively coupled contactless conductivity detection and electrospray ionization tandem mass spectrometry

Cationic isotachophoresis separation of the biomarker cardiac troponin I from a high‐abundance contaminant, serum albumin

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