Cationic isotachophoresis separation of the biomarker cardiac troponin I from a high‐abundance contaminant, serum albumin

Jacroux, Thomas; Bottenus, Danny; Rieck, Bennett T.; Ivory, Cornelius F.; Dong, Wen‐Ji

doi:10.1002/elps.201400009

Cited by 17 publications

(20 citation statements)

References 43 publications

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“…With that device, the sample with labeled cT ted human serum was examined. Cationic ITP in a straight channel was investigated le with more components in solution to mimic the situation even closer to human serum, Cl, urea, and triton X-100 [54]. However, the results did not give as high an efficiency as ously demonstrated.…”

Section: Coupled Techniquesmentioning

confidence: 98%

Exploring Gradients in Electrophoretic Separation and Preconcentration on Miniaturized Devices

Zhu

Hayes

2016

Separations

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Over the last two decades, miniaturization, integration, and automation have made microfluidic systems popular. Core to advances in microfluidics are numerous electrophoretic separation and preconcentration strategies, some finding their origins on bench-top systems. Among them, gradient-based strategies are especially effective in addressing sensitivity challenges. This review introduces several gradient-based techniques according to a broad definition, including conductivity, field, and concentration, organized by the method of gradient generation. Each technique is introduced and described, and recent seminal advances explored.

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Section: Coupled Techniquesmentioning

confidence: 98%

Exploring Gradients in Electrophoretic Separation and Preconcentration on Miniaturized Devices

Zhu

Hayes

2016

Separations

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“…Cationic ITP was applied to preconcentrate fluorescently tagged cardiac biomarker, cardiac troponin I, from model protein R‐phycoerythrin and from albumin in a PMMA straight‐channel microfluidic chip . The capillary surface was modified with high‐molecular mass branched polyethylenimine that created a low anodic EOF.…”

Section: Sample Preparationmentioning

confidence: 99%

Analysis of proteins and peptides by electromigration methods in microchips

Štěpánová

Kašička

2016

J of Separation Science

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This review presents the developments and applications of microchip electromigration methods in the separation and analysis of peptides and proteins in the period 2011-mid-2016. The developments in sample preparation and preconcentration, microchannel material, and surface treatment are described. Separations by various microchip electromigration methods (zone electrophoresis in free and sieving media, affinity electrophoresis, isotachophoresis, isoelectric focusing, electrokinetic chromatography, and electrochromatography) are demonstrated. Advances in detection methods are reported and novel applications in the areas of proteomics and peptidomics, quality control of peptide and protein pharmaceuticals, analysis of proteins and peptides in biomatrices, and determination of physicochemical parameters are shown.

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“…In addition, most microfluidic chips used for ITP are fabricated using glass and poly(methyl methacrylate) (PMMA) [ 12 , 13 ]. However, glass microfluidic chips require time-consuming and complicated wet-etching microfabrication processes that must be conducted in a cleanroom [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Optimization of Isotachophoresis Parameters for Pacific Blue Succinimidyl Ester Dye on a PDMS Microfluidic Chip

et al. 2020

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Isotachophoresis (ITP) for Pacific Blue (PB) dye using a polydimethylsiloxane (PDMS) microfluidic chip is developed and characterized by determining the types and concentrations of electrolytes, the ITP duration, and the electric field density. Among candidate buffers for the trailing electrolyte (TE) and leading electrolyte (LE), 40 mM borate buffer (pH 9) and 200 mM trisaminomethane hydrochloride (Tris-HCl) (pH 8) were selected to obtain the maximum preconcentration and resolution of the PB bands, respectively. With the selected TE and LE buffers, further optimization was performed to determine the electric field (EF) density and the ITP duration. These ITP parameters showed a 20–170,000 preconcentration ratio from initial PB concentrations of 10 nM–100 fM. Further demonstration was implemented to preconcentrate PB-conjugated lactate dehydrogenase (LDH) using the PDMS microfluidic chip. By utilizing the quenching nature of PB-LDH conjugation, we were able to identify concentrations of LDH as low as 10 ng/mL. This simple PDMS microfluidic chip-based ITP for PB preconcentration enables highly sensitive biological and chemical analyses by coupling with various downstream detection systems.

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Cationic isotachophoresis separation of the biomarker cardiac troponin I from a high‐abundance contaminant, serum albumin

Cited by 17 publications

References 43 publications

Exploring Gradients in Electrophoretic Separation and Preconcentration on Miniaturized Devices

Exploring Gradients in Electrophoretic Separation and Preconcentration on Miniaturized Devices

Analysis of proteins and peptides by electromigration methods in microchips

Characterization and Optimization of Isotachophoresis Parameters for Pacific Blue Succinimidyl Ester Dye on a PDMS Microfluidic Chip

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