Colorimetric Sensing of Hydrogen Peroxide Using Ionic‐Liquid‐Sensitized Zero‐Valent Copper Nanoparticle (nZVCu)

Abstract

In this study, a stable ionic liquid (IL) mediated zero-valent copper nanoparticle (nZVCu), namely IL-nZVCu, has been evaluated for catalytic properties towards H 2 O 2 -mediated TMB (3, 3',5,5'-tetramethylbenzidine) reaction under normal conditions of temperature and pH. The various analytical techniques like Fourier Transform Infrared Spectroscopy (FT-IR), UV-Vis diffuse reflectance spectroscopy (UV-Vis DRS), X-ray powder diffraction (XRD), scanning electron microscope (SEM), Transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS) were used to confirm the structure and nano size of nZVCu. The simple protic IL 1-hydrogen-3-methylimidazolium acetate (HmimOAc) with its cation 1-methylimidazole and anion acetate was synthesized, characterized, and coated on nZVCu to prepared IL-nZVCu. As a novel mimic peroxidase, IL-nZVCu could quickly catalyze the oxidation reaction of specific chromogenic substrate TMB, when H 2 O 2 acts as an oxidant, followed by a remarkable color change in the solution measured by UV/Vis Spectrophotometry. The enhanced catalytic activity and significant color change were greatly dependent on the catalyst dosage, the volume of IL taken, incubation time, H 2 O 2 concentration, and TMB concentration. Also, IL-nZVCu showed no response toward ascorbic acid, cholesterol, dopamine, urea and uric acid. Based on these findings, IL-nZVCu could be effectively used for visual colorimetric sensing of H 2 O 2 with a limit of detection (LOD) of 44 μM over the dynamic linear range from 40 μM to 1000 μM concentration.

Quantification of Neuronal Cell-Released Hydrogen Peroxide Using 3D Mesoporous Copper-Enriched Prussian Blue Microcubes Nanozymes: A Colorimetric Approach in Real Time and Anticancer Effect

“…The lower detection limit (LOD) was calculated for H 2 O 2 to be 6.36 nM using the equation LOD = 3σ/s and a limit of quantification (LQD = 10σ/s) of 21.21 nM where s represents the slope of the calibration curves in Figure c (inset), and σ is the standard deviation of 10 blank tests ( n = 10). Comparing these findings with previously developed methods (Table ), ,,− the detection of H 2 O 2 using the Meso-Cu-PBMCs showed a faster response time, lower LOD, and a wider linear range, indicating a higher sensitivity for H 2 O 2 detection. Additionally, the observed multicolor variation enables easy detection of H 2 O 2 even at low concentrations, providing a simple approach.…”

Ionic-Liquid-Stabilized TiO2Nanostructures: A Platform for Detection of Hydrogen Peroxide

“…An effective NP-based sensor shows a linear response to minor changes in analyte concentration Table presents a comparison of the proposed work with the previous studies reported in the literature, which gives a proof of concept that our designed sensor can work at higher as well as lower concentrations. These observations were also compared with the previous study for H 2 O 2 detection based on double molecular recognition, and the results were comparable to our proposed sensor …”

Progress in the design of portable colorimetric chemical sensing devices

“…However, CuNPs are highly susceptible to oxidation, which causes a loss in their properties and sensing ability. 66,67 So, synthesis of stabilized CuNPs is a challenging process and some researchers have synthesized stable CuNPs and used as a probe for portable colorimetric sensing application.…”