Quantification of Neuronal Cell-Released Hydrogen Peroxide Using 3D Mesoporous Copper-Enriched Prussian Blue Microcubes Nanozymes: A Colorimetric Approach in Real Time and Anticancer Effect

Madhuvilakku, Rajesh; Hong, Yonggeun; Nila, Irin Sultana; Villagra Moran, Vanina Myuriel; Subramanian, Palanisamy; Khan, Zeeshan Ahmad; Jeong, Sehoon; You, Sang Guan

doi:10.1021/acsami.3c13594

Cited by 4 publications

(2 citation statements)

References 72 publications

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“…Then, the CV currents of CuMoOx were tested with different scan rates in Figure G, and it was found that the reduction current increased with the increase in scan rate. Furthermore, the reduction peak current and square root of the scan rate showed good linearity in Figure H, indicating that the electrocatalytic reduction of H 2 O 2 by CuMoOx was controlled by diffusion . The possible mechanism of CuMoOx electrocatalytic reduction of H 2 O 2 is shown in Figure I.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, the reduction peak current and square root of the scan rate showed good linearity in Figure 3H, indicating that the electrocatalytic reduction of H 2 O 2 by CuMoOx was controlled by diffusion. 50 The possible mechanism of CuMoOx electrocatalytic reduction of H 2 O 2 is shown in Figure 3I. In the process of potential scanning from 0 to −0.…”

Section: Related Characterizations Of Nonprecious Metal Electrocataly...mentioning

confidence: 99%

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Spatial-Potential-Color-Resolved Bipolar Electrode Electrochemiluminescence Biosensor Using a CuMoOx Electrocatalyst for the Simultaneous Detection and Imaging of Tetracycline and Lincomycin

Li,

Cai,

Wang

et al. 2024

Anal. Chem.

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A spatial-potential-color-resolved bipolar electrode electrochemiluminescence biosensor (BPE-ECL) using a CuMoOx electrocatalyst was constructed for the simultaneous detection and imaging of tetracycline (TET) and lincomycin (LIN). HOF-101 emitted peacock blue light under positive potential scanning, and CdSe quantum dots (QDs) emitted green light under negative potential scanning. CuMoOx could catalyze the electrochemical reduction of H 2 O 2 to greatly increase the Faradic current of BPE and realize the ECL signal amplification. In channel 1, CuMoOx-Aptamer II (TET) probes were introduced into the BPE hole (left groove A) by the dual aptamer sandwich method of TET. During positive potential scanning, the polarity of BPE (left groove A) was negative, resulting in the electrochemical reduction of H 2 O 2 catalyzed by CuMoOx, and the ECL signal of HOF-101 was enhanced for detecting TET. In channel 2, CuMoOx-Aptamer (LIN) probes were adsorbed on the MXene of the driving electrode (DVE) hole (left groove B) by hydrogen-bonding and metal-chelating interactions. LIN bound with its aptamers, causing CuMoOx to fall off. During negative potential scanning, the polarity of DVE (left groove B) was negative and the Faradic current decreased. The ECL signal of CdSe QDs was reduced for detecting LIN. Furthermore, a portable mobile phone imaging platform was built for the colorimetric (CL) detection of TET and LIN. Thus, the multiple mode-resolved detection of TET and LIN could be realized simultaneously with only one potential scan, which greatly improved detection accuracy and efficiency. This study opened a new technology of BPE-ECL sensor application and is expected to shine in microchips and point-ofcare testing (POCT).

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Section: Resultsmentioning

confidence: 99%

Section: Related Characterizations Of Nonprecious Metal Electrocataly...mentioning

confidence: 99%

Spatial-Potential-Color-Resolved Bipolar Electrode Electrochemiluminescence Biosensor Using a CuMoOx Electrocatalyst for the Simultaneous Detection and Imaging of Tetracycline and Lincomycin

Li,

Cai,

Wang

et al. 2024

Anal. Chem.

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A spatial-potential resolved bipolar electrode electrochemiluminescence biosensor based on polarity conversion for dual-mode detection of miRNA-122 and CEA

Li,

Cai,

et al. 2024

Biosensors and Bioelectronics

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Portable Sensing Probe for Real-Time Quantification of Ammonia in Blood Samples

Madhuvilakku,

Hong

2024

ACS Appl. Mater. Interfaces

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The detection of ammonia levels in blood is critical for diagnosing and monitoring various medical conditions, including liver dysfunction and metabolic disorders. However, traditional diagnostic methods are slow and cumbersome, often involving multiple contact-based steps such as ammonia separation in alkali conditions followed by distillation or microdiffusion, leading to delays in diagnosis and treatment. Herein, we developed a colorimetric assay capable of rapid detection of ammonia in whole blood or plasma samples, utilizing 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO)-oxidized cellulose nanocrystals (TCNC) coupled with gold nanoparticles (AuNPs). The basis of our assay relies on either (i) the interaction between the carboxylate group ( − COO) of TEMPO and ammonium ions or (ii) the manipulation of AuNPs surface plasmon resonance (SPR) through the formation of Au(NH 3 ) 4 3+ , which displaces a redox mediator, resazurin, resulting in observable multicolor displays at various concentrations of ammonia. The colorimetric assay exhibits a wide linear detection range for dissolved NH 4 + (0.1−37 μM) with a low limit of detection (LOD) of 0.1 μM. Additionally, it effectively measures NH 3(g) concentrations in the range of 0.5−144 μM. The fabricated electrochemical nose (Enose) device demonstrates excellent analytical performance for plasma ammonia sensing (0.05−256 μM). Experimental results demonstrate a linear detection range suitable for clinical applications, with excellent correlation to standard laboratory methods, offering a practical solution for point-of-care (PoC) testing. We anticipate that this approach can be applied broadly to improve patient monitoring and treatment by providing immediate and accurate ammonia measurements in a clinical setting.

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Quantification of Neuronal Cell-Released Hydrogen Peroxide Using 3D Mesoporous Copper-Enriched Prussian Blue Microcubes Nanozymes: A Colorimetric Approach in Real Time and Anticancer Effect

Cited by 4 publications

References 72 publications

Spatial-Potential-Color-Resolved Bipolar Electrode Electrochemiluminescence Biosensor Using a CuMoOx Electrocatalyst for the Simultaneous Detection and Imaging of Tetracycline and Lincomycin

Spatial-Potential-Color-Resolved Bipolar Electrode Electrochemiluminescence Biosensor Using a CuMoOx Electrocatalyst for the Simultaneous Detection and Imaging of Tetracycline and Lincomycin

A spatial-potential resolved bipolar electrode electrochemiluminescence biosensor based on polarity conversion for dual-mode detection of miRNA-122 and CEA

Portable Sensing Probe for Real-Time Quantification of Ammonia in Blood Samples

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