Colorimetric Measurement of Lactic Dehydrogenase Activity of Body Fluids

Decreased H2 Histamine Response of Granulocytes of Asthmatic Patients

“…pared to histamine inhibition of BG release in five additional subjects with asthma (patients 11-15) and five additional controls (subjects [25][26][27][28][29]. At histamine concentrations of 0.01-10 ,M the inhibition of lysosomal enzyme release was again significantly less in asthma.…”

“…Total PMN enzyme concentration was assayed after cell rupture with 0.2% Triton X-100. Cell viability was determined by exclusion of trypan blue dye and assaying the concentration of the cytoplasmic enzyme lactate dehydrogenase in the supemate (28 …”

Therapeutic Benefits of Garlic against Alloxan-Induced Diabetic in Rats

“…Plasma, liver and testes alanine aminotransferase (ALT; EC 2.6.1.2) and aspartate aminotransferase (AST; EC 2.6.1.1) activities were assayed by the method of Reitman and Frankel (1957) [16] . Plasma, brain, liver and testes lactate dehydrogenase (LDH, EC1.1.1.27) activity was determined by the method of Cabaud and Wroblewski (1958) [17] . Alkaline phosphatase (AlP; EC 3.1.3.1) activity was measured at 405nm by the formation of paranitrophenol from para nitrophenyl phosphate as a substrate [18] .…”

Effect of Isoproterenol on Tissue Defense Enzymes, Hemodynamic and Left Ventricular Contractile Function in Rats

“…A 10% homogenate of heart was prepared in phosphate buffer saline (pH 7.4, 50 mM) and the aliquots of tissue homogenate were used for estimation of TBARS [9] and GSH [10]. Rest of the homogenate was cold centrifuged at 5,000 rpm for 20 min and supernatant was used for estimation of protein [11], SOD [12], CAT [13], GPx [14], CK-MB isoenzyme [15] and LDH [16].…”