Colorimetric determination of the early biomarker hypoxia-inducible factor-1 alpha (HIF-1α) in circulating exosomes by using a gold seed-coated with aptamer-functionalized Au@Au core-shell peroxidase mimic

Wang, Qian-Long; Huang, Weixue; Zhang, Pu-Juan; Chen, Li; Lio, Chon-Kit; Zhou, Hua; Qing, Lin-Sen; Luo, Pei

doi:10.1007/s00604-019-4035-z

Cited by 41 publications

(23 citation statements)

References 35 publications

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“…Rapid analysis of exosomal proteins, however, remains technically challenging due to the small size and chemical complexity of Exos 14 , 15 . Because of their high specificity, immunoassays are commonly employed to analyze exosomal proteins 16 , 17 . Among these, immuno-gold and Western-blot assays represent the gold-standard approaches for identifying specific proteins 13 , 18 , but fail to give quantitative results.…”

Section: Introductionmentioning

confidence: 99%

Nanozyme-assisted sensitive profiling of exosomal proteins for rapid cancer diagnosis

Sun

et al. 2020

Theranostics

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The proteins expressed on exosomes have emerged as promising liquid-biopsy biomarkers for cancer diagnosis. However, molecular profiling of exosomal proteins remains technically challenging. Herein, we report a nanozyme-assisted immunosorbent assay (NAISA) that enables sensitive and rapid multiplex profiling of exosomal proteins. This NAISA system is based on the installation of peroxidase-like nanozymes onto the phospholipid membranes of exosomes, thus avoiding the need for post-labelling detection antibodies. The exosomal proteins are determined by a sensitive nanozyme-catalyzed colorimetric assay less than 3 h, without the need for multi-step incubation and washing operations. Using NAISA to profile exosomal proteins from different cell lines and clinical samples, we reveal that tumor-associated exosomal proteins can serve as promising biomarkers for accurate cancer diagnosis in a cooperative detection pattern. Methods: Exosomes were engineered with DSPE-PEG-SH through hydrophobic interaction, and then were assembled with gold nanoparticles (2 nm) to produce Exo@Au nanozyme. The proteins on Exo@Au could be selectively captured by their specific antibodies seeded into a 96-well plate. The immobilized Exo@Au shows peroxidase-like activity to perform colorimetric assays by reaction with 3,3′,5,5′-tetramethylbenzidine (TMB) and H 2 O 2 . The protein levels of exosomes were recorded on a microplate reader. Results: The NAISA platform is capable of profiling multiple exosomal proteins from both cancer cell lines and clinical samples. The expression levels of exosomal proteins, such as CD63, CEA, GPC-3, PD-L1 and HER2, were used to classify different cancer cell lines. Moreover, the protein profiles have been applied to differentiate healthy donors, hepatitis B patients, and hepatic cell carcinoma (HCC) patients with high accuracy. Conclusion: The NAISA nanozyme was allowed to rapidly profile multiple exosomal proteins and could have great promise for early HCC diagnosis and identification of other cancer types.

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Section: Introductionmentioning

confidence: 99%

Nanozyme-assisted sensitive profiling of exosomal proteins for rapid cancer diagnosis

Sun

et al. 2020

Theranostics

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“…Smartphones are convenient, could be widely applied, and offer good users' experience, making them effective instruments for on-site detection [9][10][11]. To date, most smartphone-based types of colorimetric analysis were reported using the phone's camera for directly capturing images from samples [11][12][13][14]. For example, Xu [13] and our group [15] took photos with a smartphone camera for the quantitative detection of blood and urinary glucose through RGB image analysis.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Colorimetric Detection of Urine Glucose Based on Color Fading Using Smartphone Ambient-Light Sensor

Wang

Guo

et al. 2020

Chemosensors

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In this work, a label-free colorimetric assay was developed for the determination of urine glucose using smartphone ambient-light sensor (ALS). Using horseradish peroxidase—hydrogen peroxide—3,3′,5,5′-tetramethylbenzidine (HRP-H2O2-TMB) colored system, quantitative H2O2 was added to samples to-be-determined for deepest color. The presence of glucose oxidase in urine led to the formation of H2O2 and the reduction of TMBred. As a result of this, the color of the urine faded and the solution changed from deep blue to light blue. We measured the illuminance of the transmitted light by a smartphone ambient light sensor, and thereby color changes were used to calculate the content of urine glucose. After method validation, this colorimetric assay was practically applied for the determination of urine samples from diabetic patients. Good linearity was obtained in the range of 0.039–10.000 mg/mL (R2 = 0.998), and a limit of detection was 0.005 mg/mL. Our method was had high accuracy, sensitivity, simplicity, rapidity, and visualization, providing a new sensor to be potentially applicable for point-of-care detection of urine glucose.

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“…AFNs also possess these properties, having a large surface area and depending on the unique size and shape, as well as the physical and chemical properties of the composition. 64 Many different materials can attach biomolecules, such as aptamers, through a variety of surface chemical reactions. One reason for the specific fixation of the aptamer on the surface of the nanostructure may also be that the large surface area of the material increases the density of the aptamer on the material, thus increasing the area of interaction between the aptamer and the target analyte.…”

Section: Properties Of Aptamerfunctionalized Nanomaterialsmentioning

confidence: 99%

Aptamer-functionalized nanomaterials for biological applications

Ren

Lu³

et al. 2020

Mater. Chem. Front.

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Colorimetric determination of the early biomarker hypoxia-inducible factor-1 alpha (HIF-1α) in circulating exosomes by using a gold seed-coated with aptamer-functionalized Au@Au core-shell peroxidase mimic

Cited by 41 publications

References 35 publications

Nanozyme-assisted sensitive profiling of exosomal proteins for rapid cancer diagnosis

Nanozyme-assisted sensitive profiling of exosomal proteins for rapid cancer diagnosis

Label-Free Colorimetric Detection of Urine Glucose Based on Color Fading Using Smartphone Ambient-Light Sensor

Aptamer-functionalized nanomaterials for biological applications

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