Label-Free Colorimetric Detection of Urine Glucose Based on Color Fading Using Smartphone Ambient-Light Sensor

Wang, Tiantian; Guo, Kun; Hu, Xuemei; Liang, Jian; Li, Xing-De; Zhang, Zhifeng; Xie, Jing

doi:10.3390/chemosensors8010010

Cited by 22 publications

(10 citation statements)

References 26 publications

(26 reference statements)

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“…The proposed technique was found to have comparable sensitivity to the techniques of ELISA and the use of a field-effect transistor (FET). (26,27) The reversibility of the GOx activity as a function of the pH value was confirmed to be identical to previous results within the investigated pH range of the cell solution of 7.0 to 5.0. (28,29) The selectivity of the detection to other solutes is also important.…”

Section: Resultssupporting

confidence: 89%

Glucose Detection Based on pH-sensitive Liposomes

Kang¹,

Park²

2021

Sensors and Materials

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pH-sensitive liposomes were employed to amplify the detection of glucose. Glucose oxidase (GOx) covalently immobilized on magnetic particles and pH-sensitive liposomes encapsulating ferricyanide were added to a cyclic voltammeter cell solution in which glucose was distributed. The conversion of glucose into gluconic acid appeared to decrease the pH, which reduced the electrostatic repulsion between the headgroups of weakly acidic 1,2-dipalmitoyl-sn-glycero-3succinate. The reduction destabilized the liposomes, which released the potassium ferricyanide encapsulated inside them. The effects of the glucose concentration and pH value were investigated. It was necessary to add more than 10 µL of 0.5 μg/mL glucose solution to the 5 mL cyclic voltammeter cell solution to observe a response. The activity of GOx was reversible with respect to the pH change between 7 and 5. The sensitivity of this detection was almost identical to that of comparable techniques such as the use of a field-effect transistor. Therefore, the methodology developed in this study is feasible as a portable and ultrasensitive method.

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Section: Resultssupporting

confidence: 89%

Glucose Detection Based on pH-sensitive Liposomes

Kang¹,

Park²

2021

Sensors and Materials

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“…Then, 50 μL of reaction solution was withdrawn every other minute and added to 2 mL of PBS containing 10 μg of HRP and 0.1 mg of TMB, which was incubated for 30 min at 40 °C and terminated by 50 μL of 2 mol L −1 H 2 SO 4 . The absorbance was measured at 450 nm to track changes in H 2 O 2 concentration [ 35 , 36 ]. The standard curve was shown in Figure S2 .…”

Section: Methodsmentioning

confidence: 99%

Dendritic Mesoporous Silica Hollow Spheres for Nano-Bioreactor Application

Zhang

Fang

et al. 2022

Nanomaterials

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Mesoporous silica materials have attracted great research interest for various applications ranging from (bio)catalysis and sensing to drug delivery. It remains challenging to prepare hollow mesoporous silica nanoparticles (HMSN) with large center-radial mesopores that could provide a more efficient transport channel through the cell for guest molecules. Here, we propose a novel strategy for the preparation of HMSN with large dendritic mesopores to achieve higher enzyme loading capacity and more efficient bioreactors. The materials were prepared by combining barium sulfate nanoparticles (BaSO4 NP) as a hard template and the in situ-formed 3-aminophenol/formaldehyde resin as a porogen for directing the dendritic mesopores’ formation. HMSNs with different particle sizes, shell thicknesses, and pore structures have been prepared by choosing BaSO4 NP of various sizes and adjusting the amount of tetraethyl orthosilicate added in synthesis. The obtained HMSN-1.1 possesses a high pore volume (1.07 cm3 g−1), a large average pore size (10.9 nm), and dendritic mesopores that penetrated through the shell. The advantages of HMSNs are also demonstrated for enzyme (catalase) immobilization and subsequent use of catalase-loaded HMSNs as bioreactors for catalyzing the H2O2 degradation reaction. The hollow and dendritic mesoporous shell features of HMSNs provide abundant tunnels for molecular transport and more accessible surfaces for molecular adsorption, showing great promise in developing efficient nanoreactors and drug delivery vehicles.

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“…However, as previously mentioned, peroxidase (e.g., HRP) presents some disadvantages, including weak stability, small-scale production (challenging to scale up), and sensitivity to the pH and temperature (it denaturates easily). Some alternative methods have been reported for urine glucose detection based on electrochemical sensors [ 76 , 77 ], electronic nose [ 78 ], molecular-based colorimetric [ 79 ], and luminesce-based sensors [ 80 , 81 ]. Here, we only focus on colorimetric nanosensors with the potential for home-testing translation.…”

Section: Glucose Nanosensors In Different Biofluidsmentioning

confidence: 99%

Nanosensors for Visual Detection of Glucose in Biofluids: Are We Ready for Instrument-Free Home-Testing?

et al. 2021

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Making frequent large-scale screenings for several diseases economically affordable would represent a real breakthrough in healthcare. One of the most promising routes to pursue such an objective is developing rapid, non-invasive, and cost-effective home-testing devices. As a first step toward a diagnostic revolution, glycemia self-monitoring represents a solid base to start exploring new diagnostic strategies. Glucose self-monitoring is improving people’s life quality in recent years; however, current approaches still present vast room for improvement. In most cases, they still involve invasive sampling processes (i.e., finger-prick), quite discomforting for frequent measurements, or implantable devices which are costly and commonly dedicated to selected chronic patients, thus precluding large-scale monitoring. Thanks to their unique physicochemical properties, nanoparticles hold great promises for the development of rapid colorimetric devices. Here, we overview and analyze the main instrument-free nanosensing strategies reported so far for glucose detection, highlighting their advantages/disadvantages in view of their implementation as cost-effective rapid home-testing devices, including the potential use of alternative non-invasive biofluids as samples sources.

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Label-Free Colorimetric Detection of Urine Glucose Based on Color Fading Using Smartphone Ambient-Light Sensor

Cited by 22 publications

References 26 publications

Glucose Detection Based on pH-sensitive Liposomes

Glucose Detection Based on pH-sensitive Liposomes

Dendritic Mesoporous Silica Hollow Spheres for Nano-Bioreactor Application

Nanosensors for Visual Detection of Glucose in Biofluids: Are We Ready for Instrument-Free Home-Testing?

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