Color Doppler Evaluation of Isovolumetric Relaxation Time and of Signals Arising from Axons of the Median Nerve as a Means to Evaluate Mitochondrial Functionality in the Context of Immunotherapy of Cancer and Chronic Conditions Associated with Mitochondrial Dysfunction

Abstract

Reactive Oxygen Species (ROS) arising from the disruption of mitochondrial respiration act as endogenous mutagens and tumor promoters. When production of ROS exceeds the capacity of DNA repair mechanisms, random mutations and aneuploidy ensue, thousands of genes become unbalanced and genetic/epigenetic chain reactions lead to progressive aneuploidy with only two outcomes: karyotypes so altered that are not viable or karyotypes of autonomous, immortal, cancer cells. Consistent with the concept that abnormalities of cellular respiration in mitochondria precede DNA alterations in the nucleus, transplant of normal mitochondria suppresses tumorigenesis and metastases in vitro and in vivo. However, the transplant of mitochondria is an experimental procedure that cannot be easily applied to clinical practice. In order to overcome this limitation, we designed a novel formula based on microbial chondroitin sulfate, vitamin D3 and ultrapure phosphatidylcholine, molecules that are known to restore mitochondrial functionality and suppress ROS production. Here we describe how such an approach can be evaluated by color Doppler ultrasonography of the radial artery and measure of the Isovolumetric Relaxation Time (IVRT). In addition, we show for the first time color Doppler signals originating from axons of the median nerve that may be indicative of quantum phenomena at the level of mitochondria. We propose the use of the original ultrasonographic techniques here described for evaluating the effectiveness of substances or strategies aimed at restoring mitochondrial functionality at the macroscopic and quantum levels.

Consumption of an Extremely Biodiverse Probiotic and a Supplement based on Microbial Chondroitin Sulfate is Associated with Very Low Serum Alpha-N-acetylgalactosaminidase (Nagalase) Activity and Decrease of C-reactive Protein Values

“…It is therefore conceivable that the regimen adopted by Dr. MC (1 capsule of the probiotic per day and 0.1 mL of the supplement twice a week) represents the ideal combination to obtain the most benefit from the two complementary approaches. As far as the decrease of CRP is concerned, it is worth noticing that imuno ® was designed to optimize mitochondrial function as we described in a recent paper (Pacini and Ruggiero, 2019c) and it is well assessed that aging and chronic conditions are associated with, or possibly caused by, mitochondrial dysfunction that leads to chronic energy imbalance with resulting oxidative stress, systemic inflammation and elevation of CRP values (Gajewski et al, 2017). Therefore, it is plausible that optimization of mitochondrial function by imuno ® , together with the antiinflammatory properties of PMF thoroughly described in two recent articles (Pacini and Ruggiero, 2019a; was responsible for the observed decrease of CRP that was in turn responsible for the ultra-low PINI score recorded at the end of the three-month experience.…”

Experimental evidence for the role of natural radioactivity in influencing viability of commensal microorganisms

“…Static magnetic fields, ionizing, and non-ionizing radiations exert biological effects on normal and transformed cells in culture, embryos and organisms, inducing changes in signal transduction and gene expression (1–24). Certain types of eukaryotic cells, notably certain types of cancer cells (3–11), show resistance to the killing effects of ionizing radiations, a phenomenon that, in the context of radiotherapy of cancer, may represent an obstacle to effective treatment (14).…”