Consumption of an Extremely Biodiverse Probiotic and a Supplement based on Microbial Chondroitin Sulfate is Associated with Very Low Serum Alpha-N-acetylgalactosaminidase (Nagalase) Activity and Decrease of C-reactive Protein Values

Abstract

We describe the case of a 57-years-old male healthy professional who added to his nutritional plan a novel, extremely biodiverse, probiotic and a supplement based on microbial chondroitin sulfate, vitamin D 3 and ultrapure phosphatidylcholine to improve his healthy life expectancy. After three months of such a nutritional regimen, serum alpha-Nacetylgalactosaminidase (nagalase) activity was 0.40 that is a value below the minimum level established for adults (normal values: 0.50-0.95 nMol/mL/min). C-reactive Protein (CRP) was less than 1 (normal values: 0.00-5.00 mg/L), a value significantly lower than that recorded before this experience. At the end of the observation period, serum albumin level was 45 g/L, a value very close to the maximum reference value (normal values: 32.00-46.00 g/L). The Prognostic Inflammatory Nutritional Index (PINI) score was 0.05, a value that is close to the minimum calculated value (normal values: 0.00-1.00) and suggests a very low risk of death for all causes. Observation in silico and experiments in vitro demonstrated that both the probiotic and the supplement formed complexes with human nagalase with an efficiency 100 fold higher than that of purified Gc proteinderived Macrophage Activating Factor (GcMAF). The collection of these observations suggests that the combination of this probiotic and this supplement may lead to overall beneficial effects on health as evidenced by positive changes of indicators of immune system function and healthy aging such as nagalase, CRP, albumin and PINI score.

Effects on the immune system of a three-month consumption of an extremely diverse probiotic yogurt: decrease of serum alpha-N-acetylgalactosaminidase activity, detoxification and gut microbiota normalization

“…Reduction of serum nagalase activity is consistent with the observation that the extremely biodiverse probiotic yogurt described in this article has high Gc protein-derived Macrophage Activating Factor activity as demonstrated in recent studies (2,4). In addition to the effects on modulation of macrophage activity, it is worth noting that the probiotic yogurt used in this experience is highly fermented.…”

“…2). Nagalase is a marker for inflammation often found elevated in viral infections, cancer and other chronic conditions (4,11,12). These results are consistent with previous observations (3,4); we hypothesize that decrease of serum nagalase activity is due to the presence in the probiotic yogurt of high levels of naturally formed Gc protein-derived Macrophage Activating Factor that binds and neutralize nagalase (4,13)…”

“…The experience of the subject suggests that the consumption of an extremely biodiverse probiotic yogurt described in references 1 and 2 is associated increased urinary excretion of toxicants, modulation of the immune system, and rebalance of the gut microbiota with overall positive effects on health. The authors wish to emphasize that the data in this case report are from one individual, and not part of a larger study even though they confirm and expand the results observed in other, unrelated, articles (3)(4)(5)(6).…”

“…Since nagalase is considered an indicator of cancer cell proliferation, viral infections as well as a marker of systemic inflammation (4,11,12,26), and since many of the toxicants described in this article are associated with increased cancer risk, immune dysfunction and chronic inflammation, it is tempting to speculate that decrease of nagalase activity represented a reduction of the risks associated with exposure to those toxicants. In other words, we hypothesize that there are two factors involved in the observed decrease of serum nagalase activity: on one side, the Gc protein-derived Macrophage Activating Factor activity of the probiotic yogurt, a mechanism thoroughly described in previous studies (4,26); on the other side, the removal of toxicants that might have been responsible for elevated nagalase activity (3).…”

“…We have previously observed and reported in two recent articles (1,2) that the consumption of an extremely biodiverse probiotic yogurt with a unique microbial composition is associated with detoxification of non-metal toxicants (3), decrease of serum alpha-Nacetylgalactosaminidase (nagalase) activity (3,4), decrease of serum C-reactive protein (CRP) (4), decrease of markers specific for multiple myeloma (5), and a dramatic decrease of viral load in Hepatitis B (6).…”